Article
Multidisciplinary Sciences
Arnaud Cannet, Camille Simon-Chane, Mohammad Akhoundi, Aymeric Histace, Olivier Romain, Marc Souchaud, Pierre Jacob, Pascal Delaunay, Darian Sereno, Philippe Bousses, Pascal Grebaut, Anne Geiger, Chantel de Beer, Dramane Kaba, Denis Sereno
Summary: This study presents a new method for identifying Glossina species using Wing Interference Patterns (WIPs). By combining deep learning algorithms with a database of WIPs representing 23 Glossina species, accurate automatic recognition is achieved.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Analytical
Roman S. Borisov, Mariya D. Matveeva, Vladimir G. Zaikin
Summary: In recent years, there has been a special focus on the use of reactive matrices in MALDI-MS, which have dual properties and have been applied in bioanalysis to reduce sample treatment time and improve sensitivity. True reactive matrices interact with molecules through exchange or addition reactions, enhancing molecular identification and profiling.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Kristine Korzow Richter, Maria C. Codlin, Melina Seabrook, Christina Warinner
Summary: Collagen peptide mass fingerprinting by MALDI-TOF-MS, also known as ZooMS, is a rapidly growing analytical technique in the fields of archaeology, ecology, and cultural heritage. It enables rapid taxonomic identification of large bone assemblages and cultural heritage objects. However, there are limitations that need to be addressed for future advancements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Weiwei Tang, Jun-Jie Shi, Wei Liu, Xu Lu, Bin Li
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was applied to discover a glycosyltransferase (GT) named PgGT1 from the root of Platycodon grandiflorum. PgGT1 was found to catalyze the synthesis of platycoside E (PE) by sequentially attaching two beta-1,6-linked glucosyl residues to the glucosyl residue at the C3 position of platycodin D (PD). The study also revealed the crucial roles of residues S273, E274, and H350 in stabilizing the sugar donor and positioning the glucose for optimal glycosylation. These findings provide insights into the biosynthetic pathway of PE and have potential implications in industrial biotransformation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Applied
Junqiao Wang, Jing Zhao, Shaoping Nie, Mingyong Xie, Shaoping Li
Summary: This review summarizes the recent research progress on MALDI mass spectrometry (MS) detection in food carbohydrates, including the improvement of ionization efficiency, the application in structure characterization of polysaccharides, and the quantitative study and mass spectrometry imaging.
Article
Biochemical Research Methods
Nhu Q. Vu, Amanda R. Buchberger, Jillian Johnson, Lingjun Li
Summary: Neuropeptides are low abundance signaling molecules that modulate physiological processes, and mass spectrometry imaging is increasingly useful in studying them. Proper tissue washes can increase detectable neuropeptides, and using a salt solution wash may affect neuropeptide detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Jessica L. Moore, Georgia Charkoftaki
Summary: This review discusses recent advances in imaging mass spectrometry, specifically matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. It explores sample preparation strategies, considerations for different analyte classes, and the use of multimodal imaging strategies. The review also examines experimental design approaches, standardization of processes, and data analysis platforms. It concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Medicine, Research & Experimental
Verena Panitz, Saga Koncarevic, Ahmed Sadik, Dennis Friedel, Tobias Bausbacher, Saskia Trump, Vadim Farztdinov, Sandra Schulz, Philipp Sievers, Stefan Schmidt, Ina Juergenson, Stephan Jung, Karsten Kuhn, Irada Pflueger, Suraj Sharma, Antje Wick, Pauline Pfaender, Stefan Selzer, Philipp Vollmuth, Felix Sahm, Andreas von Deimling, Ines Heiland, Carsten Hopf, Peter Schulz-Knappe, Ian Pike, Michael Platten, Wolfgang Wick, Christiane A. Opitz
Summary: This study utilized LC-MS/MS combined with chemical isobaric labeling to analyze serum samples from 43 recurrent glioblastoma patients and 43 healthy controls, revealing decreased levels of Trp and its metabolites in glioblastoma patients compared to controls. Higher tumor volume was associated with lower systemic metabolite levels, and lower systemic kynurenine levels correlated with worse overall survival. Additionally, analysis of scRNA-seq data showed that genes involved in Trp metabolism were expressed in various cell types in glioblastoma, with AHR activation observed in macrophages and T cells, and high AHR activity was linked to reduced overall survival in the glioblastoma TCGA dataset.
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Taiga Iwama, Kuniyuki Kano, Daisuke Saigusa, Kim Ekroos, Gerhild van Echten-Deckert, Johannes Vogt, Junken Aoki
Summary: The novel on-tissue derivatization method using Phos-tag in combination with MALDI-MSI enables visualization of low-abundance bioactive lipids like LPA and S1P in the brain, providing a simple and optimal way to reveal the spatial localization of these important molecules in tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Zhao-Qing Zeng, Wen-Ying Zhuang
Summary: This study explored the species diversity of the Nectriaceae family by examining specimens from different regions of China. Four new taxa were introduced based on morphological characteristics and DNA sequence analyses, and the differences between these new species and their close relatives were compared and discussed.
Review
Spectroscopy
Colin T. McDowell, Xiaowei Lu, Anand S. Mehta, Peggi M. Angel, Richard R. Drake
Summary: This article introduces the development of glycan imaging mass spectrometry and discusses its applications in different sample types. Glycan imaging mass spectrometry analyses broaden our understanding of the biological and clinical relevance of N-glycosylation to human disease.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Microbiology
Zhao-Qing Zeng, Wen-Ying Zhuang
Summary: This study examines specimens from Henan, Hubei, and Jiangsu Provinces in China to explore the species diversity of the genus Fusicolla, and identifies three undescribed taxa. By analyzing the morphological characteristics and DNA sequences of multiple regions, these new species are confirmed to belong to Fusicolla. The morphological features, such as aerial mycelia formation, macroconidia and microconidia characteristics, distinguish Fusicolla aeria sp. nov., Fusicolla coralloidea sp. nov., and Fusicolla filiformis sp. nov. from their close relatives. A list of previously recorded Fusicolla species in China is provided, along with a key to these taxa.
Article
Chemistry, Analytical
Margaux Fresnais, Juergen Burhenne, Walter E. Haefeli, Remi Longuespee
Summary: Desorption/ionization (DI) methods are crucial for rapid and sensitive drug quantification from solid samples. Validating these methods requires careful consideration of the specific characteristics of DI approaches compared to traditional LC-MS methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Polymer Science
Diana-Andreea Blaj, Mihaela Balan-Porcarasu, Brindusa Alina Petre, Valeria Harabagiu, Cristian Peptu
Summary: This paper explores the reaction kinetics and details of the synthesis of beta-cyclodextrin-oligolactide derivatives initiated by beta-cyclodextrin in D,L-lactide through MALDI MS analysis, comparing data with 1H NMR and studying the influence of solvents, revealing a faster reaction in specific solvents due to activation processes.
Article
Mycology
Julien Cornut, Sophie De Respinis, Mauro Tonolla, Orlando Petrini, Felix Barlocher, Eric Chauvet, Andreas Bruder
Article
Microbiology
Jasmine S. Berg, Petra Pjevac, Tobias Sommer, Caroline R. T. Buckner, Miriam Philippi, Philipp F. Hach, Manuel Liebeke, Moritz Holtappels, Francesco Danza, Mauro Tonolla, Anupam Sengupta, Carsten J. Schubert, Jana Milucka, Marcel Kuypers
ENVIRONMENTAL MICROBIOLOGY
(2019)
Letter
Infectious Diseases
M. Brackmann, S. L. Leib, M. Tonolla, N. Schurch, M. Wittwer
CLINICAL MICROBIOLOGY AND INFECTION
(2020)
Article
Parasitology
Nadja C. Wipf, Valeria Guidi, Mauro Tonolla, Michela Ruinelli, Pie Mueller, Olivier Engler
PARASITES & VECTORS
(2019)
Article
Public, Environmental & Occupational Health
Florence Fouque, Valeria Guidi, Mario Lazzaro, Damiana Ravasi, Gladys Martinetti-Lucchini, Giorgio Merlani, Mauro Tonolla, Eleonora Flacio
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2020)
Article
Microbiology
Agathe Martignier, Sophie De Respinis, Montserrat Filella, Ines Segovia-Campos, Birger Marin, Gerd Guenther, Francois Barja, Mauro Tonolla, Jean-Michel Jaquet, Michael Melkonian, Daniel Ariztegui
Article
Virology
Jakub Kubacki, Eleonora Flacio, Weihong Qi, Valeria Guidi, Mauro Tonolla, Cornel Fraefel
Article
Infectious Diseases
Pie Mueller, Lukas Engeler, Laura Vavassori, Tobias Suter, Valeria Guidi, Martin Gschwind, Mauro Tonolla, Eleonora Flacio
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Multidisciplinary Sciences
Antoine Buetti-Dinh, Michela Ruinelli, Dorota Czerski, Cristian Scapozza, Agathe Martignier, Samuele Roman, Annapaola Caminada, Mauro Tonolla
Summary: The microbiological environment near the Greina plateau in the southern Swiss Alps is characterized by highly elevated concentrations of heavy metals and a thick biofilm mainly composed of Cyanobacteria. Genes for resistance/tolerance to heavy metals, transport systems, and extracellular polymeric substances were found, suggesting a potential role in bioremediation of metal-contaminated environments.
Article
Chemistry, Multidisciplinary
Lucrezia Pardi-Comensoli, Mauro Tonolla, Andrea Colpo, Zuzanna Palczewska, Sharanya Revikrishnan, Markus Heeb, Ivano Brunner, Michel Barbezat
Summary: The objective of this project is to evaluate the potential of fungi and bacteria for depolymerizing epoxy, with the aim of developing a sustainable management of natural resources for epoxy. Results show that microbes can colonize epoxy surfaces and partially depolymerize them, suggesting promising opportunities for the study of new metabolisms capable of depolymerizing epoxy materials.
APPLIED SCIENCES-BASEL
(2022)
Article
Environmental Sciences
Damiana Ravasi, Francesca Mangili, David Huber, Laura Azzimonti, Lukas Engeler, Nicola Vermes, Giacomo Del Rio, Valeria Guidi, Mauro Tonolla, Eleonora Flacio
Summary: This study successfully predicts the establishment probability of Aedes albopictus in Switzerland through modeling and selects ten socio-environmental predictors, and produces risk maps. It provides a useful tool for authorities to intervene in higher-risk areas.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Francesco Di Nezio, Clarisse Beney, Samuele Roman, Francesco Danza, Antoine Buetti-Dinh, Mauro Tonolla, Nicola Storelli
Summary: Meromictic lakes provide a stable anoxic environment for anaerobic microorganisms, with Lake Cadagno being a habitat for phototrophic sulfur bacteria contributing significantly to the lake's total productivity. Different populations of these bacteria display varying abilities in CO2 fixation through photosynthesis and chemosynthesis, showing substantial differences in activity and abundance.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Microbiology
Jakub Kubacki, Isabelle Hardmeier, Weihong Qi, Eleonora Flacio, Mauro Tonolla, Cornel Fraefel
Summary: This study reports the full-length genome sequence of a rhabdovirus strain detected in a pool of 21 Culex pipiens and Culex torrentium mosquitos collected in southern Switzerland. The genome is 11,914 nucleotides in length and encodes five major putative open reading frames.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)