Article
Biochemistry & Molecular Biology
Shaolong Zhang, Pengzhao Shang, Kun Gao, Guomeng Zhao, Jingping Zhou, Rong Chen, Xiaoju Ning, Changying Guo
Summary: The DNA repair machinery plays a role in estrogen-dependent transactivation. The study reveals that mechanisms underlying estrogen-induced DNA damage are complex. It is found that the process of 17 beta-estradiol (E2)-induced ROS production can be divided into two phases, and oxidative DNA damage occurs at different temporal and spatial locations due to intracellular Ca2+ fluctuation and ER alpha-dependent transcription. The study also shows that DNA oxidation is not necessary for estrogen-responsive gene expression, while topoisomerase-mediated DNA strand breaks are essential in estrogen signaling.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Cong Hua, Xuanzhong Wang, Shipeng Liang, Xi Chen, Chen Li, Guangqiang You, Chongcheng Wang, Tianfei Luo, Zhenchuan Wang, Pengfei Ge
Summary: The study reveals that silibinin induces DNA double strand breaks and ROS accumulation in glioma cells through upregulating BNIP3, and these effects can be partially inhibited by the antioxidant GSH. Knockdown of BNIP3 reverses silibinin-induced ROS accumulation, suggesting that BNIP3 contributes to silibinin-induced DNA DSBs by modulating intracellular ROS levels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Plant Sciences
Sayanti De, Jismon Jose, Amita Pal, Swarup Roy Choudhury, Sujit Roy
Summary: Multiple lines of evidence indicate the important role of solar UV-B light in regulating various cellular and metabolic activities, gene expression, growth and development in plants. This study shows that low levels of UV-B significantly influence the growth and development of Vigna radiata seedlings, promoting lateral root formation and inducing DNA damage response pathway. Furthermore, low dose UV-B exposure enhances the accumulation of specific proteins and induces endoreduplication. Transcriptome analyses reveal the activation of co-regulated genes associated with DNA repair, cell cycle regulation and oxidative stress response pathways in response to low doses of UV-B.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Cell Biology
Md Akram Hossain, Yunfeng Lin, Garrett Driscoll, Jia Li, Anne McMahon, Joshua Matos, Haichao Zhao, Daisuke Tsuchimoto, Yusaku Nakabeppu, Jianjun Zhao, Shan Yan
Summary: APE2 is essential for activating the ATR DDR pathway in response to various stressful conditions in Xenopus laevis egg extracts and human pancreatic cancer cells. Inhibition of APE2 leads to increased DNA damage and sensitizes cancer cells to chemotherapy drugs, indicating its crucial role in maintaining genome integrity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kevin M. Wernke, Alina Tirla, Mengzhao Xue, Yulia Surovtseva, Fabian S. Menges, Seth B. Herzon
Summary: Colibactin is a genotoxic metabolite produced by commensal-pathogenic members of the human microbiome, potentially linked to tumorigenesis. Researchers synthesized and studied colibactin 742 (4), finding that it induces DNA interstrand-cross-links and activates DNA repair pathways, providing a new approach to study the genotoxic effects of colibactin.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Genevieve Trombly, Afaf Milad Said, Alexei P. Kudin, Viktoriya Peeva, Janine Altmueller, Kerstin Becker, Karl Koehrer, Gabor Zsurka, Wolfram S. Kunz
Summary: The study investigated the effects of hydrogen peroxide on mitochondrial DNA integrity. The results showed that hydrogen peroxide treatment resulted in DNA breaks, which were then repaired through rapid repair of single-strand breaks and degradation of double-strand breaks-generated linear fragments. Inactivation of mitochondrial DNA degradation resulted in the persistence of linear fragments in mutant cells without affecting the repair of single-strand breaks. These findings highlight the interplay between DNA repair and degradation processes, and the potential generation of somatic mitochondrial DNA deletions.
Article
Oncology
Qunsong Tan, Kaifeng Niu, Yuqi Zhu, Zixiang Chen, Yueyang Li, Mengge Li, Di Wei, Adayabalam S. Balajee, Hongbo Fang, Yongliang Zhao
Summary: The study demonstrates that RNF8 ubiquitinates RecQL4 protein, facilitating its dissociation from DSB sites and hindering the recruitment of downstream DSB repair proteins. RecQL4 also interacts with WRAP53 beta, which enhances its association with RNF8. Overall, the ubiquitination event mediated by RNF8 is essential for RecQL4's function in DSB repair.
Article
Biochemistry & Molecular Biology
Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. Walter
Summary: Research has shown that collisions between the replicative CMG helicase and nicks in DNA templates can lead to different forms of double-strand breaks, causing replisome disassembly.
Article
Immunology
Daniel de Oliveira Patricio, Greicy Brisa Malaquias Dias, Lucilene Wildner Granella, Ben Trigg, Helena Claire Teague, Dina Bittencourt, Andre Bafica, Alfeu Zanotto-Filho, Brian Ferguson, Daniel Santos Mansur
Summary: DNA-PKcs plays a role in restricting the spread of Zika virus infection in human cells by influencing the transcription of interferons and related genes. This mechanism is independent of DNA breaks.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Erwan Goy, Maxime Tomezak, Caterina Facchin, Nathalie Martin, Emmanuel Bouchaert, Jerome Benoit, Clementine de Schutter, Joe Nassour, Laure Saas, Claire Drullion, Priscille M. Brodin, Alexandre Vandeputte, Olivier Molendi-Coste, Laurent Pineau, Gautier Goormachtigh, Olivier Pluquet, Albin Pourtier, Fabrizio Cleri, Eric Lartigau, Nicolas Penel, Corinne Abbadie
Summary: Induction of second primary cancers is a rare but severe complication of curative-intent radiation therapy. Research shows that normal human or mouse dermal fibroblasts exposed to out-of-field radiation dose at the margin of the target volume do not die, but enter a long-lived senescent state, leading to the accumulation of unrepaired DNA single-strand breaks. Some of these senescent cells eventually escape cell cycle arrest and give rise to daughter cells with mutations and invasive properties. This discovery highlights the role of single-strand break-induced senescence as the mechanism of second primary cancer initiation, with potential implications for prevention.
Article
Biochemistry & Molecular Biology
Sijie Liu, Yu Hua, Jingna Wang, Lingyan Li, Junjie Yuan, Bo Zhang, Ziyang Wang, Jianguo Ji, Daochun Kong
Summary: Protection of 30 overhangs in DNA double-strand breaks (DSBs) repair is achieved through the transient formation of RNA-DNA hybrids, with RNA polymerase III (RNAPIII) responsible for synthesizing the RNA strand. CtIP and MRN nuclease activity are required for initiating RNAPIII-mediated RNA synthesis at DSBs. Reduced RNAPIII levels suppress homologous recombination (HR) and lead to genetic loss > 30 bp at DSBs.
Article
Biochemistry & Molecular Biology
Juliette Restier-Verlet, Aurelie Joubert, Melanie L. Ferlazzo, Adeline Granzotto, Laurene Sonzogni, Joelle Al-Choboq, Laura El Nachef, Eymeric Le Reun, Michel Bourguignon, Nicolas Foray
Summary: Radiation-induced bystander effects (RIBE) refer to biological events occurring in non-targeted cells. It has been found that medium transfer-induced RIBE can result in DNA double-strand breaks, while micro-irradiation-induced RIBE shows the same temporal occurrence. The effects of RIBE on surrounding tissues can be beneficial or deleterious depending on their radiosensitivity and ability to release Ca2+ ions.
Article
Biochemistry & Molecular Biology
Laurene Sonzogni, Melanie L. Ferlazzo, Adeline Granzotto, Beatrice Fervers, Laurent Charlet, Nicolas Foray
Summary: The mechanistic model RIANS from radiobiology is crucial for understanding the recognition, repair and genotoxic stress response of DNA double-strand breaks induced by radiation. This model also applies to exposure to metal ions. Our study found that the induction of DSB by pesticides depends on their concentration and the RIANS status of cells. Impaired DSB recognition and repair, leading to toxicity, can occur when the nucleo-shuttling of ATM is delayed. Additionally, the combination of certain metal ions and pesticides can have additive or supra-additive effects on DSB recognition and/or repair.
Review
Cell Biology
Ksenia G. Kolobynina, Alexander Rapp, M. Cristina Cardoso
Summary: Chromatin serves as the background for all DNA-based molecular processes in the cell nucleus. The initial chromatin structure at the site of DNA damage determines lesion generation and activation of the DNA damage response pathway. Ubiquitination, as an important chromatin post-translational modification, is involved in chromatin changes at the damaged site and throughout the genome.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Cui Wang, Kenny Ebel, Katja Heinze, Ute Resch-Genger, Ilko Bald
Summary: Photodynamic therapy (PDT) for cancer treatment relies on the generation of highly reactive oxygen species by light-induced excitation of a photosensitizer in the presence of molecular oxygen, causing DNA damage. Recently, the potential of photoactive complexes of non-noble metals as photosensitizers has been discovered.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
