Article
Multidisciplinary Sciences
Yizhe Sun, Dan Lu, Yue Yin, Jia Song, Yang Liu, Wenyan Hao, Fang Qi, Guangze Zhang, Xin Zhang, Liang Liu, Zhiqiang Lin, Hui Liang, Xuyang Zhao, Yan Jin, Yuxin Yin
Summary: PTEN is frequently mutated in human cancers and PTEN alpha, an isoform of PTEN, remains active in cancer bearing stop-gained PTEN mutations. Through counteraction of CD8(+) T cell-mediated cytotoxicity, PTEN alpha promotes T cell dysfunction and accelerates immune-resistant cancer progression. This suggests that PTEN alpha may be a potential target in antitumor immunotherapy.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Adib Behrouzi, Hanyu Xia, Eric L. Thompson, Mark R. Kelley, Jill C. Fehrenbacher
Summary: Cisplatin-induced neurotoxicity is a common complication of anti-cancer treatment. Inhibition of OGG1 and APE1 endonuclease activity exacerbates oxidative DNA damage and neurotoxicity in sensory neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Bahareh Tabanifar, Anbalagan Moorthy, Heng Hang Tsai, Srinivasaraghavan Kannan, Chandra S. Verma, Kanaga Sabapathy
Summary: This study reveals a mechanism of cell death mediated by JNK, involving the degradation of the anti-apoptotic protein APE1. Upon exposure to genotoxic stress, JNK interacts with APE1, leading to its ubiquitination and degradation, which ultimately triggers cell death.
Article
Biochemistry & Molecular Biology
Melanie Ragon, Lucie Bertheau, Jennifer Dumont, Tiffany Bellanger, Marie Grosselin, Mohini Basu, Eleonore Pourcelot, Walid Horrigue, Emmanuel Denimal, Ambroise Marin, Basile Vaucher, Antoine Berland, Corentin Lepoivre, Sebastien Dupont, Laurent Beney, Hazel Davey, Stephane Guyot
Summary: This study investigates the influence of fluorescent proteins on the microbial stress response. The results show that the presence of Green Fluorescent Protein (GFP) enhances the resistance of Saccharomyces cerevisiae to heat shock and affects the cultivability under oxidative stresses. These findings highlight the importance of fluorescent proteins in the microbial stress response.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2023)
Article
Engineering, Environmental
Preeti Singh, Prasenjit Mitra, Taru Goyal, Shailja Sharma, Praveen Sharma
Summary: Occupational exposure to heavy metals like lead and cadmium may result in increased oxidative DNA damage and impaired expression of the DNA repair gene.
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
(2021)
Review
Biochemistry & Molecular Biology
Lauren Sahakian, Ainsley M. Robinson, Linda Sahakian, Rhian Stavely, Mark R. Kelley, Kulmira Nurgali
Summary: Inflammatory bowel disease (IBD) is a chronic relapsing inflammation of the gastrointestinal tract. The prevalence of IBD is increasing worldwide, calling for the development of new treatments. APE1/Ref-1 has been identified as a potential target for IBD therapy due to its role in regulating crucial pathways in inflammatory diseases. This review discusses the current status of IBD treatments, the role of APE1/Ref-1 in intestinal inflammation, and the potential of small molecule inhibitors to modulate inflammation and oxidative stress in IBD.
Article
Multidisciplinary Sciences
Silvia Martini, Khalil Davis, Rupert Faraway, Lisa Elze, Nicola Lockwood, Andrew Jones, Xiao Xie, Neil Q. McDonald, David J. Mann, Alan Armstrong, Jernej Ule, Peter J. Parker
Summary: PKC epsilon regulates the mitotic events by controlling translation and promoting the assembly of ribonucleoprotein granules containing SERBP1, which is essential for chromosome segregation and successful cell division independent of Aurora B. The authors demonstrate that SERBP1 acts as a parallel mitotic PKC epsilon substrate ensuring the integrity of chromosome segregation and successful cell division.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Nina Komaniecka, Marta Porras, Louis Cairn, Jon Ander Santas, Nerea Ferreiro, Juan Carlos Penedo, Sonia Banuelos
Summary: APE1 is a key enzyme in the BER pathway, involved in repairing oxidative DNA damage. Its structure includes a catalytic domain and a flexible N-terminal extension. Conformational rearrangements occur internally and between molecules when APE1 binds DNA and interacts with NPM1. These findings suggest that the N-terminal region approaches the DNA near the abasic site, and its spatial configuration is sensitive to NPM1, potentially impacting APE1's regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Sarah Hofmann, Nancy Kedersha, Paul Anderson, Pavel Ivanov
Summary: Stress granules are membrane-less ribonucleoprotein-based cellular compartments that form in the cytoplasm in response to environmental stressors. Their function is not fully understood, but aberrant formation and impaired disassembly contribute to various pathological conditions. Recent studies suggest a novel mechanism for stress granule assembly involving a network of mRNAs and proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Wentao Wang, Ying Ma, Miaoling Huang, Weichu Liang, Xingqi Zhao, Qianwen Li, Shiwei Wang, Zhigang Hu, Lingfeng He, Tao Gao, Jinfei Chen, Feiyan Pan, Zhigang Guo
Summary: It has been discovered that under oxidative stress, c-Myc is a downstream target of OGG1 and H4R3me2a is involved in the transcriptional regulation, promoting gene transcription.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Max Baymiller, Stephanie L. Moon
Summary: Stress granules (SGs) are biomolecular condensates that form during global translation suppression. They sequester translation factors and mRNAs, potentially remodeling the functional transcriptome during stress. Understanding SGs is important due to their implication in various diseases.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Multidisciplinary Sciences
Ming Zhao, Yu Yu, Li-Ming Sun, Jia-Qing Xing, Tingting Li, Yunkai Zhu, Miao Wang, Yin Yu, Wen Xue, Tian Xia, Hong Cai, Qiu-Ying Han, Xiaoyao Yin, Wei-Hua Li, Ai-Ling Li, Jiuwei Cui, Zhenghong Yuan, Rong Zhang, Tao Zhou, Xue-Min Zhang, Tao Li
Summary: RNA triggers liquid-liquid phase separation (LLPS) of the SARS-CoV-2 nucleocapsid protein N, confirming its role in infection. Chemical compound (-)-gallocatechin gallate (GCG) disrupts N's LLPS and inhibits viral replication, suggesting a potential treatment option for COVID-19.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Konstantinos Mazarakos, Huan-Xiang Zhou
Summary: In this study, a mean-field theoretical model and molecular dynamics simulations are used to demonstrate that the multiphase organization of multi-component condensates undergoes a second phase transition, driven by the difference in attraction strengths between different components. The phase transitions are observed by decreasing the temperature, leading to condensate separation from the bulk phase and component demixing within the condensates. Molecular dynamics simulations of model mixtures verify the existence of a critical temperature for demixing and predicted binodals.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Su He, Hongwei Gou, Yulin Zhou, Chunxiu Wu, Xinxin Ren, Xiajunpeng Wu, Guanwen Guan, Boxing Jin, Jinhua Huang, Zhigang Jin, Tiejun Zhao
Summary: Viruses use various strategies to suppress the host's innate immune response for viral replication and pathogenesis. The SARS-CoV-2 nucleocapsid protein can inhibit the innate immune response and facilitate viral replication by inducing atypical foci.
Article
Multidisciplinary Sciences
Ilya Shabanov, J. Ross Buchan
Summary: Quantification of cellular structures is important for understanding cellular function, but detecting and quantifying different cellular structures can be challenging. To address this, we developed HARLEY, a customizable software that automatically detects and quantifies stress granules in yeast, providing consistent results.
SCIENTIFIC REPORTS
(2022)
Article
Hematology
Capucine Siberchicot, Nathalie Gault, Nathalie Dechamps, Vilma Barroca, Adriano Aguzzi, Paul-Henri Romeo, J. Pablo Radicella, Anne Bravard, Jacqueline Bernardino-Sgherri
Article
Genetics & Heredity
Sumedha M. Kondekar, Gaurav Gunjal, Juan Pablo Radicella, Desirazu N. Rao
Article
Biochemistry & Molecular Biology
Emilie Lebraud, Guillaume Pinna, Capucine Siberchicot, Jordane Depagne, Didier Busso, Damiano Fantini, Lamya Irbah, Elena Robeska, Gueorgui Kratassiouk, Jean-Luc Ravanat, Bernd Epe, J. Pablo Radicella, Anna Campalans
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Natalia Rubanova, Guillaume Pinna, Jeremie Kropp, Anna Campalans, Juan Pablo Radicella, Anna Polesskaya, Annick Harel-Bellan, Nadya Morozova
Article
Multidisciplinary Sciences
Lamya El Mortaji, Alejandro Tejada-Arranz, Aline Rifflet, Ivo G. Boneca, Gerard Pehau-Arnaudet, J. Pablo Radicella, Stephanie Marsin, Hilde De Reuse
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Jacqueline Bernardino-Sgherri, Capucine Siberchicot, Frederic Auvre, Didier Busso, Clementine Brocas, Ghazi El Masri, Anna Lioutsko, Federica Ferri, J. Pablo Radicella, Paul-Henri Romeo, Anne Bravard
Summary: The study reveals that in various solid tumors, the expression of PrPC increases after exposure to ionizing radiation, and PrPC deficiency enhances the sensitivity of tumor cells to radiation. Further experiments demonstrate that radioresistance mediated by the ATM-TAK1-PrPC pathway is activated in different tumor cell lines.
Article
Multidisciplinary Sciences
Laure Etourneaud, Angela Moussa, Emilie Rass, Diane Genet, Simon Willaume, Caroline Chabance-Okumura, Paul Wanschoor, Julien Picotto, Benoit Theze, Jordane Depagne, Xavier Veaute, Elea Dizet, Didier Busso, Aurelia Barascu, Lamya Irbah, Thierry Kortulewski, Anna Campalans, Catherine Le Chalony, Sophie Zinn-Justin, Ralph Scully, Gaelle Pennarun, Pascale Bertrand
Summary: The interaction between lamin B1 and 53BP1 is crucial for DNA damage repair, and overexpression of lamin B1 can lead to repair defects and increased sensitivity to DSBs.
Article
Multidisciplinary Sciences
Prashant P. Damke, Louisa Celma, Sumedha M. Kondekar, Anne Marie Di Guilmi, Stephanie Marsin, Jordane Depagne, Xavier Veaute, Pierre Legrand, Helene Walbott, Julien Vercruyssen, Raphael Guerois, Sophie Quevillon-Cheruel, J. Pablo Radicella
Summary: The ComFC protein is essential for natural transformation and is involved in transporting DNA through the cell membrane and handling single-stranded DNA in the cytoplasm. The crystal structure of ComFC and its affinity for single-stranded DNA suggest that it plays a crucial role in the recombination machinery.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Sonia Abdallah, Amandine Jampy, Delphine Moison, Margaux Wieckowski, Sebastien Messiaen, Emmanuelle Martini, Anna Campalans, Juan Pablo Radicella, Virginie Rouiller-Fabre, Gabriel Livera, Marie-Justine Guerquin
Summary: Many endocrine disruptors, including bisphenols, impair the meiotic process and oocyte quality. This study investigates the effects of two bisphenol alternatives, BADGE and BPAF, on oogenesis in mice. The compounds delay meiosis initiation, induce DNA damage, and negatively affect gene expression in fetal premeiotic germ cells. These findings provide insights into the mechanisms underlying the deleterious effects of bisphenols and suggest oxidative DNA damage as a common mechanism for environmental pollutants.
ENVIRONMENTAL POLLUTION
(2023)
Article
Cell Biology
Xhaferr Tanushi, Guillaume Pinna, Marie Vandamme, Capucine Siberchicot, Ostiane D'Augustin, Anne-Marie Di Guilmi, J. Pablo Radicella, Bertrand Castaing, Rebecca Smith, Sebastien Huet, Francois Leteurtre, Anna Campalans
Summary: 8-oxoG is a mutagenic DNA lesion induced by ROS, and OGG1 plays a major role in repairing it. Two competitive inhibitors of OGG1, TH5487 and SU0268, have been identified. Our study uncovered two off-target effects of these inhibitors, inhibiting ABC family transporters and interfering with metaphase completion. These effects should be considered when interpreting studies using TH5487 and SU0268.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sandrine Ragu, Nathalie Droin, Gabriel Matos-Rodrigues, Aurelia Barascu, Sylvain Caillat, Gabriella Zarkovic, Capucine Siberchicot, Elodie Dardillac, Camille Gelot, Josee Guirouilh-Barbat, J. Pablo Radicella, Alexander A. Ishchenko, Jean-Luc Ravanat, Eric Solary, Bernard S. Lopez
Summary: In human primary cells, a noncanonical cellular response specific to nonblocking replication stress is discovered. This response generates reactive oxygen species (ROS) and activates detoxification genes. ROS are produced by DUOX1/DUOX2 and controlled by NF-kappa B.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Ostiane D'Augustin, Virginie Gaudon, Capucine Siberchicot, Rebecca Smith, Catherine Chapuis, Jordane Depagne, Xavier Veaute, Didier Busso, Anne-Marie Di Guilmi, Bertrand Castaing, J. Pablo Radicella, Anna Campalans, Sebastien Huet
Summary: The DNA-glycosylase OGG1 constantly samples the DNA by rapidly alternating between diffusion within the nucleoplasm and short transits on the DNA, which is crucial for its recruitment to oxidative lesions. The sampling process is tightly regulated by the residue G245, while the residues Y203, N149, and N150 differentially regulate the sampling of the DNA and recruitment to oxidative lesions based on previous structural data.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Oncology
Shohei Murakami, Vilma Barroca, Leila Perie, Anne Bravard, Jacqueline Bernardino-Sgherri, Amandine Tisserand, Caroline Devanand, Valerie Edmond, Aurelie Magniez, Sabrina Tenreira Bento, Claire Torres, Florence Pasquier, Isabelle Plo, William Vainchenker, Jean-Luc Villeval, Paul-Henri Romeo, Daniel Lewandowski
Summary: This study develops a mouse model to study the progression of polycythemia vera (PV) and identifies carbonic anhydrase 1 (CA1) as a potential therapeutic target for PV.
BLOOD CANCER DISCOVERY
(2022)
Article
Biotechnology & Applied Microbiology
C. F. M. Menck, R. S. Galhardo, A. Quinet
Summary: Studies have shown that xeroderma pigmentosum variant (XP-V) patients have mutations in the POLH gene, resulting in a high frequency of skin tumors. However, it is paradoxical that the translesion synthesis DNA polymerase eta (Pol η) in these patients can actually suppress mutations, and the mechanism behind this is still unclear. Recent evidence suggests that cyclobutane pyrimidine dimers (CPDs) play an instructional role for Pol η, enabling accurate replication of these lesions, and the mutagenic effects induced by UV radiation are caused by the deamination of C-containing CPDs. This process leads to C>T transitions, which are the most common mutations in skin cancers. The delayed replication in XP-V cells amplifies the deamination of C in CPDs and increases the burden of C>T mutations through the activity of backup TLS polymerases.
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
(2024)