Article
Chemistry, Multidisciplinary
Tereza Cervena, Andrea Rossnerova, Tana Zavodna, Jitka Sikorova, Kristyna Vrbova, Alena Milcova, Jan Topinka, Pavel Rossner
Summary: The evaluation of genotoxicity induced by nanomaterials in bronchial epithelial cells using different variants of the micronuclei assay showed that the DNA damage level is affected by the selected methodological approach. Transmission electron microscopy demonstrated the impact of cytochalasin-B on the transportation of NMs into cells, while dynamic light scattering measurement revealed the behavior of NMs in culture media. The genotoxic potential of various TiO2 and Ag particles differed, with the application of cytochalasin-B tending to increase the percentage of aberrant cells.
Article
Toxicology
Ji-Eun Seo, Xilin Li, Yuan Le, Nan Mei, Tong Zhou, Xiaoqing Guo
Summary: This study compared the performance of HepaRG cells grown in 2D and 3D cultures in the high-throughput flow cytometry-based micronucleus (MN) assay. The results showed that 3D HepaRG spheroids exhibited higher sensitivity in detecting genotoxicants requiring metabolic activation. Integration of the MN and comet assays improved the sensitivity for detecting such genotoxicants, suggesting that HepaRG spheroids may contribute to New Approach Methodologies for genotoxicity assessment.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Environmental Sciences
Milda Babonaite, Matas Cepulis, Jurate Kazlauskaite, Juozas Rimantas Lazutka
Summary: According to PlasticEurope, global plastics production reached 390.7 million tons in 2021. Unfortunately, a large portion of these plastics end up as waste in the ocean or on land due to their non-biodegradable nature. This study focused on evaluating the internalization rates, cytotoxicity, and genotoxicity of polystyrene nanoparticles (PS-NPs) in human peripheral blood mononuclear cells (PBMCs) in vitro. The results showed that while PS-NPs did not have a cytotoxic effect on PBMCs, they did induce primary DNA damage and micronucleated cells, indicating their genotoxic potential.
Article
Food Science & Technology
Miroslav Misik, Armen Nersesyan, Michael Kment, Benjamin Ernst, Tahereh Setayesh, Franziska Ferk, Klaus Holzmann, Georg Krupitza, Siegfried Knasmueller
Summary: The micronucleus (MN) test using Huh6 cells shows promising results with a sensitivity of 80% and a specificity of 94% for the detection of genotoxic carcinogens. The validity of MN tests with Huh6 is higher than that of other experimental models, which suggests a potential reduction in the use of laboratory rodents in genetic toxicology studies.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Environmental Sciences
Ronan Adler Tavella, Caroline Lopes Feijo Fernandes, Lucia Emanueli Schimith, Lisiane Martins Volcao, Marina dos Santos, Flavio Manoel Rodrigues da Silva Junior
Summary: Socioeconomic and demographic factors, lifestyle, and cultural characteristics may have an important impact on genetic damage. This study analyzed the association between a set of factors and genetic damage by studying populations in southern Brazil. The results showed that age had a weak association with genetic damage, while factors such as skin color, occupational exposure, drug use, exposure to radiation, and the cultural habit of consuming chimarrao were found to be associated with genetic damage. Yerba mate consumption was identified as a confounding factor in cytogenetic studies in the southern region of South America.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Plant Sciences
Hussin Muhammad, Maizatul Hasyima Omar, Elda Nurafnie Ibnu Rasid, Shazlan Noor Suhaimi, Shazlan Noor Suhaimi Farah Huda Mohkiar, Lau Mei Siu, Norizah Awang
Summary: The present study evaluated the genotoxicity potential of Ficus deltoidea var. kunstleri aqueous extract (FDAE) using standard in vitro assays. The results showed that FDAE at certain concentrations could cause DNA damage, but did not significantly affect micronucleus formation or revertant colonies in bacteria. Further investigation in animal models is necessary to confirm the non-genotoxicity activities of FDAE.
Article
Genetics & Heredity
Alexander R. Vernon, Roy M. Pemberton, H. Ruth Morse
Summary: This study aims to improve the prediction of genotoxicity by using a novel 3D in vitro assay that replicates the in vivo bone marrow microenvironment. Compared to the standard 2D assay, this method shows more accurate prediction of the genotoxicity of glucocorticoids in vivo.
Article
Plant Sciences
Ioanna Efthimiou, Dimitris Vlastos, Vassilios Triantafyllidis, Antonios Eleftherianos, Maria Antonopoulou
Summary: This study investigated the genotoxic and cytotoxic activity of Betula pendula leaf extract and found that it exhibited antigenotoxic potential. The mixture of the extract with mitomycin-C showed significant antigenotoxic activity at low concentrations.
Article
Chemistry, Physical
S. Garcia-Carpintero, V. Jehova Gonzalez, J. Frontinan-Rubio, A. Esteban-Arranz, E. Vazquez, M. Duran-Prado
Summary: In recent years, the genotoxicity of graphene-related materials (GRMs) and other 2D materials has been evaluated using different models. The OECD TG 487 in vitro micronucleus (MNu) test is commonly used to assess the genotoxicity of nanomaterials (NMs). However, the nature of NMs interferes with the standard MNu process, requiring adaptations. The study proposes a modified in vitro MNu test with improvements to accurately test the genotoxicity of NMs, demonstrating risks associated with graphene oxide (GO) and boron nitride (hBN).
Article
Chemistry, Multidisciplinary
Fatima Pinto, Ana Filipa Lourenco, Jorge F. S. Pedrosa, Lidia Goncalves, Celia Ventura, Nadia Vital, Ana Bettencourt, Susete N. Fernandes, Rafaela R. da Rosa, Maria Helena Godinho, Henriqueta Louro, Paulo J. T. Ferreira, Maria Joao Silva
Summary: Cellulose micro/nanomaterials (CMNM) are promising bio-nanomaterials with natural and renewable source, attractive properties, and potential for industrial and economical applications. This study evaluated the cell internalization and in vitro cytotoxicity and genotoxicity of CMNM compared to multi-walled carbon nanotubes (MWCNT). The results showed that CMNM did not induce cytotoxic effects, while MWCNT did. In terms of genotoxicity, some components of CMNM also exhibited significant genotoxicity.
Article
Pharmacology & Pharmacy
Raffaella di Vito, Mattia Acito, Cristina Fatigoni, Carl H. Schiesser, Michael J. Davies, Francesca Mangiavacchi, Milena Villarini, Claudio Santi, Massimo Moretti
Summary: This study evaluated the cytotoxic and genotoxic potential of SeTal in liver cells through in vitro experiments. The results showed that SeTal had minimal impact on cell viability and did not cause clastogenic or aneugenic activity. These findings suggest that SeTal may be a potential active pharmaceutical ingredient.
Article
Biochemistry & Molecular Biology
Andreja Juric, Irena Brcic Karaconji, Nevenka Kopjar
Summary: The study found that HGA in strawberry tree honey posed no significant cytotoxic threat to human peripheral blood lymphocytes in vitro, had a negligible potential for causing cytogenetic damage in treated cells, and efficiently counteracted the detrimental cytogenetic effects of the antineoplastic drug irinotecan. Further research is needed to explore the safety profile of HGA and its potential for developing new nutraceutical products.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Toxicology
Jingjing Wan, Ruixue Chen, Zhou Yang, Jing Xi, Yiyi Cao, Yu Chen, Xinyu Zhang, Yang Luan
Summary: Although AA IVa can form DNA adducts in vitro, it is non-genotoxic in vivo due to its low reactivity and transformation into easily excreted metabolites.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Environmental Sciences
Claudia Galeano-Paez, Pedro Espitia-Perez, Luisa Jimenez-Vidal, Karina Pastor-Sierra, Shirley Salcedo-Arteaga, Luz Stella Hoyos-Giraldo, Adriana Gioda, Tatiana Dillenburg Saint'Pierre, Solange Cristina Garcia, Hugo Brango, Lyda Espitia-Perez
Summary: The study found that residents of La Mojana region showed significant increase in cytogenetic damage and total Hg levels in hair, which were related to high fish consumption. High fish consumption and T-Hg contents were also correlated with necrotic and apoptotic cell frequencies. Additionally, exposure to Hg induced genetic instability and increased micronuclei frequencies in individuals who consumed certain fish species with carnivorous habits.
Article
Plant Sciences
Debora Elisa Antunes de Mendonca, Mariane Aparecida Franco de Godoy, Naiara Cristina Lucredi, Jurandir Fernando Comar, Igor Vivian Almeida, Veronica Elisa Pimenta Vicentini
Summary: This study evaluated the effects of Ganoderma lucidum fruiting body dry extract (GLE) on human liver and kidney tumor cells as well as peripheral blood lymphocytes. The results showed that low concentrations of GLE increased lymphocyte viability while high concentrations were toxic to lymphocytes. On the other hand, low concentrations of GLE exhibited cytotoxic activity and induced DNA damage in tumor cells. Therefore, G. lucidum has potential antitumor effects and could be developed into a therapeutic agent for cancer.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)