Article
Immunology
Jan-Hendrik Schroeder, Gordon Beattie, Jonathan W. W. Lo, Tomasz Zabinski, Nick Powell, Joana F. F. Neves, Richard G. G. Jenner, Graham M. M. Lord
Summary: Significant progress has been made in understanding innate lymphoid cells (ILC) by applying concepts from T cell biology, such as flow cytometry gating strategies and CD90 markers. Surprisingly, a sub-population of ILC in the gut were found to have low or no expression of CD90, contrary to expectations. These CD90-negative and CD90-low CD127(+) ILC were influenced by stimulatory cues and dysbiosis, and could produce IL-13, IFN gamma and IL-17A in steady state and colitis conditions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Douglas E. Weidemann, James Holehouse, Abhyudai Singh, Ramon Grima, Silke Hauf
Summary: Gene expression inherently gives rise to stochastic variation (noise) in the production of gene products. Minimizing noise is crucial for reliable cellular functions. In this study, the researchers demonstrate that several cell division genes in fission yeast exhibit mRNA variances significantly below the assumed limit of Poissonian noise, which is explained by a gene expression model incorporating multiple transcription and mRNA degradation steps. This finding redefines the lower limit of eukaryotic gene expression noise and uncovers molecular requirements for achieving ultralow noise.
Article
Oncology
Alexander C. Dowell, Haydn Munford, Anshita Goel, Naheema S. Gordon, Nicholas D. James, K. K. Cheng, Maurice P. Zeegers, Douglas G. Ward, Richard T. Bryan
Summary: The study found widespread expression of PD-L2 in urothelial bladder cancer and normal urothelium, with significantly increased expression in advanced disease, contrary to decreased expression in muscle-invasive bladder cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Amira Seltana, Gabriel Cloutier, Vilcy Reyes Nicolas, Taoufik Khalfaoui, Inga C. Teller, Nathalie Perreault, Jean-Francois Beaulieu
Summary: Fibrinogen plays an important role in wound healing and tissue inflammation, acting as a substrate in the basement membrane of intestinal cells. It has a promoting effect on cell healing, especially under pathological conditions that require rapid epithelial repair.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Hanna Abberger, Romy Barthel, Jasmin Bahr, Jacqueline Thiel, Sina Luppus, Jan Buer, Astrid M. Westendorf, Wiebke Hansen
Summary: The study found that in activated CD4(+) non-Tregs, Nrp-1 expression has an activating role, while in CD4(+) non-Tregs from naive mice, Nrp-1 expression leads to dysfunction.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Pegah Mehrpouya-Bahrami, Alina K. Moriarty, Paulo De Melo, W. Coles Keeter, Nada S. Alakhras, Andrew S. Nelson, Madeline Hoover, Maria S. Barrios, Jerry L. Nadler, C. Henrique Serezani, Mark H. Kaplan, Elena Galkina
Summary: STAT4 is essential for immunity to intracellular pathogens and plays a key role in neutrophil functions, including ROS production, chemotaxis, and formation of neutrophil extracellular traps. Deletion of STAT4 results in enhanced susceptibility to MRSA, highlighting its crucial involvement in innate immune responses.
Article
Pharmacology & Pharmacy
Cullen M. Lilley, Andrea Alarcon, My-Huyen Ngo, Jackeline S. Araujo, Luis Marrero, Kimberlee S. Mix
Summary: The orphan nuclear receptor 4A2 (NR4A2/Nurr1) plays an important role in the development of rheumatoid arthritis (RA) and is overexpressed in inflamed synovium. The expression of NR4A2 and its upstream regulator, nuclear factor kappa B (NF-kappa B), increases during disease progression, early expressed in inflamed synovium. Screening of RA-related genes revealed that NR4A receptors and NF-kappa B-1 play central regulatory roles in this model.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Veterinary Sciences
Francesca De Falco, Antonella Perillo, Fabio Del Piero, Chiara Del Prete, Nicola Zizzo, Ioan Marcus, Sante Roperto
Summary: This study reveals that Eras is a functional gene in horses and ERAS is constitutively expressed in adult horse tissues. ERAS plays a physiological role in maintaining cellular proteostasis, reducing the toxicity of accumulated misfolded proteins, and contributing to protection against diseases.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Tomoya Kitakaze, Rina Tatsumi, Mayu Yamaguchi, Aino Nakatsuji, Naoki Harada, Ryoichi Yamaji
Summary: This study found that the expression of LGR6 gene transiently increased before the myogenic regulatory genes during the differentiation of C2C12 myoblasts into myotubes. Loss of LGR6 decreased differentiation and fusion indices, while exogenous expression of LGR6 promoted myogenic differentiation. Additionally, the transient expression of LGR6 gene required retinoic acid and its downregulation was regulated by the ubiquitin-proteasome system involving ZNRF3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Amanda Goulart, Medeton Mahoussi Michael Boko, Nubia Sabrina Martins, Ana Flavia Gembre, Romulo Silva de Oliveira, Sandra Patricia Palma-Albornoz, Thais Bertolini, Paulo Eduardo Martins Ribolla, Leandra Naira Zambelli Ramalho, Thais Fernanda de Campos Fraga-Silva, Vania Luiza Deperon Bonato
Summary: IL-22 plays a pro-inflammatory role in allergic asthma, contributing to the recruitment and activation of eosinophils. The absence of IL-22 reduces eosinophil number and cytokine production, as well as mucus production and pulmonary inflammation. Transfer of Th17 cells from IL-22 deficient mice also affects eosinophil frequency in the airways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pathology
Julie A. Vrana, Jennifer M. Boland, Simone B. S. P. Terra, Hao Xie, Sarah M. Jenkins, Aaron S. Mansfield, Julian R. Molina, Stephen D. Cassivi, Anja C. Roden
Summary: SATB2 expression was higher in small cell carcinomas compared to carcinoid tumors in the lung, while it was expressed more in atypical carcinoid tumors and large cell neuroendocrine carcinoma than small cell carcinoma in the thymus. SATB2 may not be a reliable marker to identify the origin of neuroendocrine tumors and further validation studies are needed.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2021)
Article
Neurosciences
Isabel Gross, Nicola Brandt, Danara Vonk, Franziska Koeper, Lars Woehlbrand, Ralf Rabus, Martin Witt, Axel Heep, Torsten Ploesch, Mark S. Hipp, Anja U. Braeuer
Summary: This study analyzes the expression and localization of PRG5 in the mouse brain, and finds its association with dendritic spine formation and stabilization. PRG5 is highly expressed in areas with regenerative capacity and specifically expressed in the late phase of adult neurogenesis.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Erin H. Doyle, Costica Aloman, Ahmed El-Shamy, Francis Eng, Adeeb Rahman, Arielle L. Klepper, Brandy Haydel, Sander S. Florman, M. Isabel Fiel, Thomas Schiano, Andrea D. Branch
Summary: The study found that certain NK cells in the liver of HCV-infected patients were correlated with better liver function. Higher concentration of IL-10 in portal vein blood may prime inhibitory pathways, attenuating TLR signaling. Hepatic NK cells may play a critical role in maintaining liver homeostasis during HCV infection.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Jonas Feldheim, David Wend, Mara J. Lauer, Camelia M. Monoranu, Martin Glas, Christoph Kleinschnitz, Ralf-Ingo Ernestus, Barbara M. Braunger, Patrick Meybohm, Carsten Hagemann, Malgorzata Burek
Summary: This study found that high expression of PCDHGC3 is associated with longer progression-free survival (PFS) in glioma patients. Knockout of PCDHGC3 decreased the growth rate but increased the migration rate of glioma cells, and also affected the expression of genes involved in the WNT signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pathology
Sofia Garces, Zbigniew Rudzki, C. Cameron Yin, Roberto N. Miranda, Ana M. Medina, Vathany Sriganeshan, Branko Cuglievan, Shaoying Li, Jie Xu, Fatima Jelloul, Joseph D. Khoury, Beenu Thakral, Guilin Tang, Juan C. Garces, L. Jeffrey Medeiros
Summary: Dermatopathic lymphadenopathy (DL) is a type of lymph node hyperplasia that occurs in chronic dermatologic diseases, and it usually resolves without specific therapy. This study found high expression of MUM1/IRF4 in all tested DL cases and suggested its utility in highlighting DL dendritic cells and distinguishing DL from other histiocytic and Langerhans cell lesions.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2022)
Article
Immunology
Laurence Bouchet-Delbos, Amandine Even, Emilie Varey, Soraya Saiagh, Sylvain Bercegeay, Cecile Braudeau, Brigitte Dreno, Gilles Blancho, Regis Josien, Maria-Cristina Cuturi, Aurelie Moreau
Summary: The study developed a GMP-compliant method for producing ATDCs and compared the phenotype and functions of ATDCs extracted from healthy volunteers and patients.
Article
Cell Biology
Leen Catrysse, Bastiaan Maes, Parul Mehrotra, Arne Martens, Esther Hoste, Liesbet Martens, Christian Maueroder, Anneleen Remmerie, Anna Bujko, Karolina Slowicka, Mozes Sze, Hanna Vikkula, Bart Ghesquiere, Charlotte L. Scott, Yvan Saeys, Bart van de Sluis, Kodi Ravichandran, Sophie Janssens, Geert van Loo
Summary: The study found that myeloid-specific A20-deficient mice are protected from diet-induced obesity and insulin resistance despite the presence of an inflammatory environment in their metabolic tissues. Macrophages lacking A20 show impaired mitochondrial respiratory function and metabolize more palmitate, resulting in a lean phenotype and protection from metabolic disease. These findings suggest a role for A20 in regulating macrophage immunometabolism.
Review
Immunology
Christian Zwicker, Anna Bujko, Charlotte L. Scott
Summary: With the advancement of single cell technologies, research has shifted towards exploring the heterogeneity of hepatic macrophages, revealing the presence of distinct subsets in the liver. This heterogeneity has raised questions about the plasticity of macrophages, leading to investigations into whether differential responses are due to the presence of multiple subsets in different environments.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Martin Guilliams, Johnny Bonnardel, Birthe Haest, Bart Vanderborght, Camille Wagner, Anneleen Remmerie, Anna Bujko, Liesbet Martens, Tinne Thone, Robin Browaeys, Federico F. De Ponti, Bavo Vanneste, Christian Zwicker, Freya R. Svedberg, Tineke Vanhalewyn, Amanda Goncalves, Saskia Lippens, Bert Devriendt, Eric Cox, Giuliano Ferrero, Valerie Wittamer, Andy Willaert, Suzanne J. F. Kaptein, Johan Neyts, Kai Dallmeier, Peter Geldhof, Stijn Casaert, Bart Deplancke, Peter ten Dijke, Anne Hoorens, Aude Vanlander, Frederik Berrevoet, Yves Van Nieuwenhove, Yvan Saeys, Wouter Saelens, Hans Van Vlierberghe, Lindsey Devisscher, Charlotte L. Scott
Summary: This study presents a spatial proteogenomic atlas of the liver, combining multiple omics datasets, and reveals the cellular niches and transcriptomic identities of Kupffer cells and lipid-associated macrophages. It also demonstrates the inducibility of lipid-associated macrophages by local lipid exposure and the crucial role of ALK1-BMP9/10 axis in Kupffer cell development.
Article
Biochemistry & Molecular Biology
Mathieu Uzzan, Jerome C. Martin, Luka Mesin, Alexandra E. Livanos, Tomas Castro-Dopico, Ruiqi Huang, Francesca Petralia, Giuliana Magri, Shashi Kumar, Qing Zhao, Adam K. Rosenstein, Minami Tokuyama, Keshav Sharma, Ryan Ungaro, Roman Kosoy, Divya Jha, Jeremy Fischer, Harpriya Singh, Mary E. Keir, Nandhini Ramamoorthi, William E. O' Gorman, Benjamin L. Cohen, Adeeb Rahman, Francesca Cossarini, Akihiro Seki, Louise Leyre, Sonia Tejedor Vaquero, Sakteesh Gurunathan, Emilie K. Grasset, Bojan Losic, Marla Dubinsky, Alexander J. Greenstein, Zoe Gottlieb, Peter Legnani, James George, Haritz Irizar, Aleksandar Stojmirovic, Carrie Brodmerkel, Andrew Kasarkis, Bruce E. Sands, Glaucia Furtado, Sergio A. Lira, Zewen K. Tuong, Huaibin M. Ko, Andrea Cerutti, Charles O. Elson, Menna R. Clatworthy, Miriam Merad, Mayte Suarez-Farinas, Carmen Argmann, Jason A. Hackney, Gabriel D. Victora, Gwendalyn J. Randolph, Ephraim Kenigsberg, Jean Frederic Colombel, Saurabh Mehandru
Summary: The study revealed highly dysregulated B cell response in UC patients, with major alterations in colonic B cells, reduced clonal diversity of plasma cells, and the potential of circulating gut-homing plasmablasts as a biomarker for disease activity.
Editorial Material
Immunology
Pieter A. Louwe, Charlotte L. Scott
Summary: The transcription factor c-MAF plays a crucial role in regulating perivascular macrophage phenotypes, particularly in white adipose tissue, where its loss from murine vasculature-associated macrophages leads to increased vascularization and protection from metabolic syndrome.
SCIENCE IMMUNOLOGY
(2021)
Editorial Material
Immunology
Federico F. De Ponti, Charlotte L. Scott
Summary: Hypoxia during acute respiratory distress syndrome alters populations of monocytes and macrophages, leading to persistent inflammation, which can be reversed by therapeutic administration of CSF1.
Article
Medicine, General & Internal
Nicolas Chapelle, Malgorzata Osmola, Jerome Martin, Justine Blin, Maxime Leroy, Iva Jirka, Driffa Moussata, Dominique Lamarque, Raphael Olivier, David Tougeron, Anne Hay-Lombardie, Edith Bigot-Corbel, Damien Masson, Jean-Francois Mosnier, Tamara Matysiak-Budnik
Summary: Pepsinogen testing by chemiluminescent enzyme immunoassay is accurate for the detection of corpus atrophic gastritis, while IL-6 and HE-4 may be beneficial for the detection of antrum atrophic gastritis.
Editorial Material
Medicine, Research & Experimental
Federico F. De Ponti, Charlotte L. Scott
Summary: Researchers investigated the potential therapeutic effects of AnxA1 treatment on NASH progression, finding that it can reduce inflammation and fibrosis in NASH mice without affecting steatosis or metabolic syndrome. The therapeutic effects may be achieved through modulating macrophage populations in the liver.
Article
Biochemical Research Methods
Isabelle Scheyltjens, Hannah Van Hove, Karen De Vlaminck, Daliya Kancheva, Jonathan Bastos, Monica Vara-Perez, Ana Rita Pombo Antunes, Liesbet Martens, Charlotte L. Scott, Jo A. Van Ginderachter, Yvan Saeys, Martin Guilliams, Niels Vandamme, Kiavash Movahedi
Summary: This protocol provides a detailed workflow for single-cell multi-omic analysis of the mouse brain immune compartment. It includes steps for brain dissection, cell dissociation, high-dimensional flow cytometry, droplet-based single-cell RNA sequencing, and analysis of the data. This optimized workflow allows for a comprehensive assessment of immune cell heterogeneity and activation in the brain, which is crucial for studying neurological disorders.
Editorial Material
Immunology
Kathryn Waller, Charlotte L. Scott
Summary: IRF8 plays a crucial role in the development of conventional dendritic cell type I (cDC1), even in cells that have already committed to the cDC1 lineage. Deletion of IRF8 leads to loss of commitment and reprogramming of the cells towards a cDC2-like phenotype.
TRENDS IN IMMUNOLOGY
(2022)
Review
Immunology
Martin Guilliams, Charlotte L. Scott
Summary: Single-cell and spatial transcriptomic technologies have uncovered the heterogeneity of liver macrophages, which is crucial for understanding their roles in liver homeostasis and disease.
Article
Oncology
John A. Grout, Philemon Sirven, Andrew M. Leader, Shrisha Maskey, Eglantine Hector, Isabelle Puisieux, Fiona Steffan, Evan Cheng, Navpreet Tung, Mathieu Maurin, Romain Vaineau, Lea Karpf, Martin Plaud, Anne-Laure Begue, Koushik Ganesh, Jeremy Mesple, Maria Casanova-Acebes, Alexandra Tabachnikova, Shilpa Keerthivasan, Alona Lansky, Jessica Le Berichel, Laura Walker, Adeeb H. Rahman, Sacha Gnjatic, Nicolas Girard, Marine Lefevre, Diane Damotte, Julien Adam, Jerome C. Martin, Andrea Wolf, Raja M. Flores, Mary Beth Beasley, Rachana Pradhan, Soren Muller, Thomas U. Marron, Shannon J. Turley, Miriam Merad, Ephraim Kenigsberg, Helene Salmon
Summary: This study identifies two populations of cancer-associated fibroblasts (CAF) in lung tumors that are associated with T-cell exclusion. These populations orchestrate a specific structural tissue organization and produce distinct matrix molecules. The findings highlight the importance of targeting these CAF populations to increase immunotherapy efficacy in patients with T cell-excluded tumors.
Article
Immunology
Aurelie Fantou, Eric Lagrue, Thomas Laurent, Laurence Delbos, Stephanie Blandin, Anne Jarry, Gaelle Beriou, Cecile Braudeau, Nina Salabert, Eros Marin, Aurelie Moreau, Juliette Podevin, Arnaud Bourreille, Regis Josien, Jerome C. Martin
Summary: This research further characterizes the production and distribution of IL-22BP in Crohn's disease (CD) tissues. IL-22BP levels were found to be higher in inflamed ileums than colons. Mononuclear phagocytes and eosinophils expressed the highest levels of IL-22BP among T cell sources. Inflamed colons showed increased IL-22/IL-22BP ratios and a strong correlation between IL-22BP and CCL24. Monocyte-derived dendritic cells (moDC) were identified as a cellular subtype co-expressing both IL-22BP and IL-22, and retinoic acid induced the secretion of IL-22BP and CCL24 by moDC. Higher IL-22BP levels were also observed in active smokers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Bart Vanderborght, Kevin De Muynck, Eva Gijbels, Sander Lefere, Charlotte L. Scott, Martin Guilliams, Alain Beschin, Mathieu Vinken, Xavier Verhelst, Anja Geerts, Hans Van Vlierberghe, Lindsey Devisscher
Summary: Due to the rapid progression of the disease and the lack of curative treatments, hepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide. While monocyte-derived tumor-associated macrophages are known to contribute to HCC, the role of Kupffer cells (KCs) remains unclear. This study used a mouse model to investigate the effect of KC depletion on HCC initiation and progression, and found that the temporary ablation of resident KCs did not impact HCC pathogenesis.
INTERNATIONAL JOURNAL OF CANCER
(2023)
