Article
Biochemistry & Molecular Biology
Sameer Quazi
Summary: The CRISPR Cas9 genome editing tool has significant potential in cellular immunotherapy, overcoming limitations of CAR and TCR cell therapies. It has the ability to improve cancer treatment and enhance the immune response.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Si-Tong Lv, Kai Gao, Hak Myong Choe, Zheng-Yun Jin, Shuang-Yan Chang, Biao-Hu Quan, Xi-Jun Yin
Summary: This study investigated the effect of Myostatin (MSTN) on the superior rectus (SR) and superior oblique (SO) muscles in pigs. The results showed that MSTN-/- pigs had significantly larger muscle fiber cross-sectional areas compared to wild-type (WT) pigs, and the fiber type in MSTN-/- SO exhibited greater changes compared to SR. Furthermore, the expression of MyoD and MyoG genes was significantly increased in MSTN-/- SO.
ANIMAL BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Nicolas Gonzalez Castro, Jan Bjelic, Gunya Malhotra, Cong Huang, Salman Hasan Alsaffar
Summary: Recent advances in programmable nucleases have driven genome editing from research to clinical and industrial applications. Each technology has its distinct characteristics and safety is a paramount consideration, with comprehensive off-target detection strategies being crucial for ensuring safety in all applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Wilfried A. Kues, Dharmendra Kumar, Naresh L. Selokar, Thirumala Rao Talluri
Summary: Emerging and modern gene engineering techniques, such as zinc finger nucleases, transcription activator-like effector nucleases, and CRISPR/Cas9, have rapidly advanced the application of stem cell technology in disease modeling and regenerative medicine. These genome editing tools allow for precise manipulation of genes, uncovering the genetics and etiology of diseases and offering promising therapeutic solutions for genetic mutations and incurable diseases.
CURRENT STEM CELL RESEARCH & THERAPY
(2022)
Review
Oncology
Gayathri A. Kanu, Javad B. M. Parambath, Raed O. Abu Odeh, Ahmed A. Mohamed
Summary: Gold nanoparticles (AuNPs) have emerged as versatile drug-delivery nanostructures in gene therapy, especially for the treatment of monogenic diseases. Their unique properties enable efficient gene editing and gene silencing, making them promising tools for future research in this field.
Article
Medicine, Research & Experimental
Zifeng Cui, Hui Liu, Hongfeng Zhang, Zhaoyue Huang, Rui Tian, Lifang Li, Weiwen Fan, Yili Chen, Lijie Chen, Sen Zhang, Bhudev C. Das, Konstantin Severinov, Inga Isabel Hitzeroth, Priya Ranjan Debata, Zhuang Jin, Jiashuo Liu, Zheying Huang, Weiling Xie, Hongxian Xie, Bin Lang, Ji Ma, Haiyan Weng, Xun Tian, Zheng Hu
Summary: This study compared the performance of ZFNs, TALENs, and SpCas9 in HPV16 and found that SpCas9 was more efficient and specific compared to ZFNs and TALENs.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Computer Science, Artificial Intelligence
Chakkarai Sathyaseelan, L. Ponoop Prasad Patro, Thenmalarchelvi Rathinavelan
Summary: Zinc finger (ZnF) is an important class of nucleic acid and protein recognition domain with diverse structures and biological functions. In order to accurately predict ZnF motifs, researchers collected 74 ZnF sequence patterns and used Pfam HMM profiles for prediction. They developed a web server called ZnF-Prot that can predict 31 ZnF domains in protein sequences of any organism.
PATTERN RECOGNITION
(2023)
Article
Multidisciplinary Sciences
Ranjeet Maurya, Aparna Swaminathan, Uzma Shamim, Smriti Arora, Pallavi Mishra, Aakarshan Raina, Varsha Ravi, Bansidhar Tarai, Sandeep Budhiraja, Rajesh Pandey
Summary: The emergence of multiple SAR-CoV-2 variants during the COVID-19 pandemic has led to an increased understanding of the host-immune kinetics associated with these variants. A longitudinal investigation revealed a robust antiviral response in Omicron infection, which was largely absent in the Pre-VOC and Delta waves. The study also highlighted differential protein-protein interactions and gene expression patterns, particularly in relation to zinc finger proteins, that contribute to the immune response.
Article
Cell Biology
Bin Yang, Haiyu Tang, Nan Wang, Jian Gu, Qin Wang
Summary: Abnormal hypermethylation of the ZNF334 gene, which downregulates its expression, was detected in CRC tissues. TET1 was identified to be involved in regulating the methylation level of ZNF334. A constructed targeted demethylation system successfully upregulated ZNF334 expression and inhibited CRC growth.
CELL DEATH & DISEASE
(2023)
Review
Pediatrics
Nur Atikah Zakaria, Rosnah Bahar, Wan Zaidah Abdullah, Abdul Aziz Mohamed Yusoff, Shaharum Shamsuddin, Ridhwan Abdul Wahab, Muhammad Farid Johan
Summary: Beta-thalassemia, a common hematologic disease, can be cured only through allogenic hematopoietic stem cell transplantations, but the difficulty of finding matched donors limits the availability of this treatment option. However, novel therapies such as RNA manipulation, genome editing, and induced pluripotent stem cells offer potential alternatives for the treatment of beta-thalassemia.
FRONTIERS IN PEDIATRICS
(2022)
Article
Fisheries
Michael Coogan, Veronica Alston, Baofeng Su, Karim Khalil, Ahmed Elaswad, Mohd Khan, Rhoda M. C. Simora, Andrew Johnson, De Xing, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Tasnuba Hasin, Jeffery Terhune, Ian A. E. Butts, Rex A. Dunham
Summary: This study investigated the effects of CRISPR/Cas9 knockout of the myostatin gene in channel catfish. The results showed that gene-edited channel catfish exhibited high mutation and inheritance rates, as well as improved growth and disease resistance compared to control groups. Therefore, the use of MSTN gene-edited channel catfish is of great importance for commercial farms.
Review
Agronomy
Hiroshi Hisano, Fumitaka Abe, E. Robert Hoffie, Jochen Kumlehn
Summary: The recent development of customizable endonucleases has significantly advanced genetic engineering, providing unprecedented precision and efficiency for targeted gene modifications. Among these technologies, RNA-guided CRISPR-associated Cas endonucleases stand out as the most powerful tool for genome editing applications.
Article
Multidisciplinary Sciences
Yifang Mao, Xingyu Jiang, Peng Guo, Ying Ouyang, Xiangfu Chen, Meng Xia, Lixin Wu, Zihao Tang, Tianyi Liang, Yue Li, Mian He
Summary: This study identifies significant overexpression of zinc finger, X-linked, duplicated family member C (ZXDC) in metastatic cervical cancer (CC) tissue, which promotes CC cell metastasis by activating the IGF2BP3/RhoA/ROCK pathway.
Review
Genetics & Heredity
Ahmed AbdulJabbar Suleiman, Walaa Yahya Saedi, Mohammed Jobair Muhaidi
Summary: Cancer is a disease that is feared worldwide, with high mortality rates highlighting the need for more effective treatments. Gene editing technology using engineered nucleases offers a promising approach for modifying target genes, potentially revolutionizing cancer therapy.
Review
Biology
Abdurrahman Pharmacy Yusuf, Murtala Bello Abubakar, Ibrahim Malami, Kasimu Ghandi Ibrahim, Bilyaminu Abubakar, Muhammad Bashir Bello, Naeem Qusty, Sara T. Elazab, Mustapha Umar Imam, Athanasios Alexiou, Gaber El-Saber Batiha
Summary: Zinc was established more than half a century ago as an essential micronutrient for normal human physiology, with around 10% of the human proteome potentially binding zinc. Proteins with zinc-binding domains play key roles in epigenetic modifications, such as DNA methylation and histone modifications, regulating transcription under physiological and pathological conditions. Zinc metalloproteins in epigenetics, mainly zinc metalloenzymes and zinc finger proteins, interact as writers, erasers, readers, editors, and feeders to maintain the epigenome's functioning.
Article
Chemistry, Analytical
Yan Ding, Yu Zhang, Xiaoqi Shi, Lingling Liao, Qinghe Li, Tingmei Duan, Hao Jiang, ZhongXiang Ding, Hongyan Wang, Honglin Liu
Summary: A mild, cost-effective, and non-toxic strategy using iodide etching was developed to directly estimate the amount of DNA on gold nanoparticles (GNPs). This strategy allows for easy quantification of DNA using fluorescence recovery after incubation with the etching reagent. It offers a breakthrough in DNA quantification methods and has important applications in bio-detection assays and nanoparticle-based therapeutics.
ANALYTICAL METHODS
(2022)
Article
Optics
Xida Li, Jianhong Shi, Xiaoyan Wu, Honglin Liu, Yiwei Sun, Hao Sun, Guihua Zeng, Juan Huo, Hao Lei
Summary: By utilizing deep learning and the quantum nature of photons, we can reconstruct images with only one single-shot detection under ultra-low photon count, providing a new flexible approach for low photon count imaging.
APPLIED PHYSICS B-LASERS AND OPTICS
(2022)
Article
Chemistry, Multidisciplinary
Hao Wang, Zheng Cao, Liqin Zheng, Xian Wang, Mengke Su, Honglin Liu
Summary: The dimerization of PNTP to DMAB is possibly driven directly by the laser heating effect. In plasmonic photocatalysis, the conversion rate and reaction rate are highly dependent on the size of the gold nanoparticles. The reaction is not only driven by hot electron transfer, but also to a large extent by photothermal conversion. This extraordinary evidence sheds new light on the puzzle of plasmonic photochemical reactions.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Fanfan Yu, Zheng Cao, Shiyu He, Hui Xiang, Gan Zhao, Lina Yang, Honglin Liu
Summary: Highly luminescent and functionalized gold nanocluster nano-assembled structures were fabricated through poly-l-arginine surface engineering for improved luminescence. These assemblies were used for imaging glutathione molecules in cells and living organisms with low background and high sensitivity.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Baobao Chen, Mingtian Deng, Meng-Hao Pan, Shao-Chen Sun, Honglin Liu
Summary: In this study, the researchers found that fertilization-dependent ERK1/2 plays an important role in zygotic reprogramming and early embryonic development in mice. Inhibition of ERK1/2 led to abnormal accumulation of H3K9me2, 5mC, and its oxides, resulting in increased asymmetry of parental chromatin. Furthermore, the inhibition of ERK1/2 triggered abnormal localization of G9a and Tet3, leading to impaired paternal chromatin binding. The findings suggest that fertilization-dependent ERK1/2 regulates zygotic H3K9me2 and 5mC, contributing to the developmental competence of pre-implantation embryos.
CELL AND BIOSCIENCE
(2022)
Article
Pharmacology & Pharmacy
Jiyi Liang, Wei Li, Honglin Liu, Xiaofen Li, Chuqiao Yuan, Wenjun Zou, Liping Qu
Summary: DXXK may have a synergistic effect with ATO in lowering LDL-C and preventing atherosclerosis through the SREBP2/PCSK9 pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Instruments & Instrumentation
Honglin Liu, Puxiang Lai, Jingjing Gao, Zhentao Liu, Jianhong Shi, Shensheng Han
Summary: In this study, a new perspective is proposed to interpret the mechanism of information transmission through scattering media. The scattering medium is conceptualized as an assembly of randomly packed pinhole cameras, and the concept is demonstrated through simulation and experiments. The efficiency of different channels is also analyzed.
Article
Food Science & Technology
Bei Wang, Qing Meng, Lin Xiao, Ruili Li, Chunhai Peng, Xueli Liao, Jingna Yan, Honglin Liu, Guanhua Xie, Chi-Tang Ho, Huarong Tong
Summary: This study analyzed the volatile compounds in Pu-erh ripen tea to uncover the sources of its unique taste and aroma. The results showed that methoxyphenyl compounds were the dominant components responsible for the aroma of Pu-erh ripen tea, with six specific compounds contributing to the aging aroma, and others contributing to the wood-like and floral aromas. Methoxyphenyl compounds play a vital role in the unique flavor of Pu-erh ripen tea.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Cell Biology
Xinying Zhou, Hui Xu, Qianna Li, Qi Wang, Honglin Liu, Yingqi Huang, Yao Liang, Linmiao Lie, Zhenyu Han, Yaoxin Chen, Yulan Huang, Wenle Zhou, Qian Wen, Chaoying Zhou, Shengfeng Hu, Li Ma
Summary: Viperin plays a role in regulating the activation and function of dendritic cells during Mycobacterium tuberculosis infection, and this effect is mediated through NF-kappa B p65 activation pathway. Viperin deficiency enhances the phagocytic activity and clearance ability of dendritic cells against Mtb, and also enhances the interaction between dendritic cells and T cells.
INFLAMMATION RESEARCH
(2023)
Article
Obstetrics & Gynecology
Jianhui Yang, Aitong Xu, YuMin Zhang, Jiahui Deng, Xuemei Lin, Lili Xie, Xiaochun Deng, Honglin Liu, Peishan Chen, Yuejun Huang
Summary: This study, which includes 345 pregnant women, explores the relationship between prenatal depression and small for gestational age (SGA) birth. The findings suggest that changes in methylation levels in the placenta may be involved in this relationship.
BMC PREGNANCY AND CHILDBIRTH
(2022)
Article
Chemistry, Physical
Guopeng Fan, Honglin Liu, Chaochao Liu, Yanhua Xue, Zihao Ju, Sha Ding, Yuling Zhang, Yuanbo Li
Summary: This study investigates the use of waste seashells as modified materials to prepare high-performance modified asphalt in order to solve the problems of waste seashells causing environmental pollution and resource waste in China. The research findings indicate that seashell powder and stratum corneum-exfoliated seashell powder can be physically compatible with asphalt. Furthermore, the stratum corneum-exfoliated seashell powder shows better improvement in water stability and high-temperature deformation resistance of asphalt, while its effect on low-temperature performance is less favorable.
Article
Food Science & Technology
Wei Wei, Chengwan Zha, Aiwen Jiang, Zhe Chao, Liming Hou, Honglin Liu, Ruihua Huang, Wangjun Wu
Summary: Skeletal muscle fiber types can affect pork quality parameters. This study identified key proteins regulating muscle fiber types in pigs using proteomics and transcriptome data, and explored potential regulatory mechanisms through functional enrichment and protein-protein interaction analysis.
Article
Environmental Sciences
Hengfang Wang, Honglin Liu, Tianhong Yang, Guanghui Lv, Wenjing Li, Yuncai Chen, Deyan Wu
Summary: This study elucidated the responses and potential functions of soil microbial communities during succession in alpine mining areas. The results showed that mining disturbance significantly reduced the relative abundance of archaea, while restoration increased bacterial network node connections and decreased archaeal network connections. The networks of microbial genes related to nitrogen and phosphorus cycle potential varied depending on the succession type.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Green & Sustainable Science & Technology
Guodong Li, Hongzhi Wang, Zhaoxuan Liu, Honglin Liu, Haitian Yan, Zenwei Liu
Summary: The gob-side entry retaining (GER) technique, a member of the pillarless coal mining system, is gaining popularity due to its high resource recovery rate and environmental benefits. The development of the modified high-water backfill material (MBM), which is mixed with aeolian sand, provides a cost-effective solution for coal operators in arid areas. The MBM exhibits high strength, adjustable setting time, and increased peak and residual strength with the addition of aeolian sand.
Article
Geochemistry & Geophysics
Zhiwen Chen, Honglin Liu, Chengyu Zhu, Shuqi Ma, Yinjian Hang, Wenjie Luo
Summary: This study investigates the effects of stress and seepage disturbance caused by coal seam mining on the properties of weakly consolidated rock. The results show that mining-induced stress promotes fracture development rate and decreases permeability and hysteresis of the rock with increasing clay mineral content.
