期刊
MOLECULES AND CELLS
卷 33, 期 2, 页码 111-116出版社
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
DOI: 10.1007/s10059-012-2164-x
关键词
bidirectional promoter; co-expression; gene regulation; non-coding RNA; Pcna
资金
- National Research Foundation of Korea (NRF) [2008-0058561 (2010-0000155), 2010-0026759]
- Rural Development Administration (RDA), Korea [20070401-034-030]
- National Research Foundation of Korea [2010-0026759] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
AK007836 encodes a noncoding RNA (ncRNA) consisting of 2 exons. Since AK007836 is located just upstream of Pcna and transcribed in the opposite direction to that of Pcna, we analyzed its expression pattern. Both ncRNA and Pcna expressions were detected in in vitro and in vivo cells, showing a positive correlation. A 177 bp region separating the first exons of Pcna and AK007836 has a bidirectional promoter activity. When the expression of ncRNA was reduced by siRNA, Pcna expression was also reduced in normal cells, but not in cancer cells. These results suggest that the ncRNA is divergently transcribed from the bidirectional promoter, positively regulating the neighboring protein-coding Pcna gene transcription, and this regulatory function is somehow disrupted in cancer cells.
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