期刊
MOLECULES AND CELLS
卷 33, 期 3, 页码 277-283出版社
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
DOI: 10.1007/s10059-012-2252-y
关键词
A549; microarray; microRNA; paclitaxel; tumor initiating cells
资金
- National Natural Science Foundation of China [81071786, 81172070]
- Military Clinical high-tech grant [2010gxjs 070]
- National High Technology Research and Development Program of China [2008 AA02Z104]
Increasing evidence demonstrates that miRNAs are involved in the dysregulation of tumor initiating cells (TICs) in various tumors. Due to a lack of definitive markers, cell sorting is not an ideal separation method for lung adenocarcinoma initiating cells. In this study, we combined paclitaxel with serum-free medium cultivation (inverse-induction) to enrich TICs from A549 cells, marked by CD133/CD326, defined features of stemness. We next investigated aberrant microRNAs in this subpopulation compared to normal cells with miRNA microarray and found that 50 miRNAs exhibited a greater than 2-fold change in expression. As further validation, 10 miRNAs were chosen to perform quantitative RT-PCR on the A549 cell line and primary samples. The results suggest that aberrant expression of miRNAs such as miR-29ab, miR-183, miR-175p and miR-127-3P may play an important role in regulating the bio-behavior of TICs.
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