期刊
MOLECULES
卷 23, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/molecules23092245
关键词
depsidones; Aspergillus unguis; chemical induction; plasma-induced mutant; docking
资金
- Yangfan Talent Project of Guangdong Province [201433009]
- Natural Science Foundation of Guangdong Province [2018A030307046]
- Free Exploration Project of the Shenzhen Fund for Basic Research [JCYJ20170306165013264]
- Program for Scientific Research Start-Up Funds of Guangdong Ocean University (GDOU) [E15155]
- Applied S & T Research and Development Major Project of Guangdong Province [2016B020235001]
- China Postdoctoral Science Foundation [2012T50258]
- Scientific Fund of the Zhanjiang Municipal Oceanic and Fishery Bureau [A18018]
A new depsidone derivative (1), aspergillusidone G, was isolated from a marine fungus Aspergillus unguis, together with eight known depsidones (2-9) and a cyclic peptide (10): agonodepside A (2), nornidulin (3), nidulin (4), aspergillusidone F (5), unguinol (6), aspergillusidone C (7), 2-chlorounguinol (8), aspergillusidone A (9), and unguisin A (10). Compounds 1-4 and 7-9 were obtained from the plasma induced mutant of this fungus, while 5, 6, and 10 were isolated from the original strain under chemical induction. Their structures were identified using spectroscopic analysis, as well as by comparison with literature data. The HPLC fingerprint analysis indicates that chemical induction and plasma mutagenesis effectively influenced the secondary metabolism, which may be due to their regulation in the key steps in depsidone biosynthesis. In bioassays, compound 9 inhibited acetylcholinesterase (AChE) with IC50 in 56.75 mu M. Compounds 1, 5, 7, 8, and 9 showed moderate to strong activity towards different microbes. Compounds 3, 4, and 5 exhibited potent larvicidality against brine shrimp. In docking studies, higher negative CDOCKER interaction energy and richer strong interactions between AChE and 9 explained the greater activity of 9 compared to 1. Chemical induction and plasma mutagenesis can be used as tools to expand the chemodiversity of fungi and obtain useful natural products.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据