Antifungal Plant Defensins: Mechanisms of Action and Production

Solid-phase peptide synthesis (SPPS), C-terminal vs. side-chain anchoring: a reality or a myth

(2014) Prabhakar Cherkupally et al.   AMINO ACIDS

Agp2p, the Plasma Membrane Transregulator of Polyamine Uptake, Regulates the Antifungal Activities of the Plant Defensin NaD1 and Other Cationic Peptides

(2014) M. R. Bleackley et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions

(2014) Fung T Lay et al.   BMC PLANT BIOLOGY

Plant Antimicrobial Peptides Snakin-1 and Snakin-2: Chemical Synthesis and Insights into the Disulfide Connectivity

(2014) Paul W. R. Harris et al.   CHEMISTRY-A EUROPEAN JOURNAL

Experimental Conversion of a Defensin into a Neurotoxin: Implications for Origin of Toxic Function

(2014) Shunyi Zhu et al.   MOLECULAR BIOLOGY AND EVOLUTION

Specific domains of plant defensins differentially disrupt colony initiation, cell fusion and calcium homeostasis inNeurospora crassa

(2014) Alberto Muñoz et al.   MOLECULAR MICROBIOLOGY

The use of versatile plant antimicrobial peptides in agribusiness and human health

(2014) Elizabete de Souza Cândido et al.   PEPTIDES

Synthesis and Biological Properties of Fungal Glucosylceramide

(2014) Maurizio Del Poeta et al.   PLoS Pathogens

Phosphoinositide-mediated oligomerization of a defensin induces cell lysis

(2014) Ivan KH Poon et al.   eLife

Development of New Thioester Equivalents for Protein Chemical Synthesis

(2013) Ji-Shen Zheng et al.   ACCOUNTS OF CHEMICAL RESEARCH

Identification and Mechanism of Action of the Plant Defensin NaD1 as a New Member of the Antifungal Drug Arsenal against Candida albicans

(2013) Brigitte M. E. Hayes et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Expanding the landscape of recombinant protein production in Escherichia coli

(2013) Alejandro Hochkoeppler   BIOTECHNOLOGY LETTERS

Properties and mechanisms of action of naturally occurring antifungal peptides

(2013) Nicole L. van der Weerden et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

The yeast sphingolipid signaling landscape

(2013) David J. Montefusco et al.   CHEMISTRY AND PHYSICS OF LIPIDS

Synthesis of PAF, an Antifungal Protein fromP. chrysogenum, by Native Chemical Ligation: Native Disulfide Pattern and Fold Obtained upon Oxidative Refolding

(2013) Györgyi Váradi et al.   CHEMISTRY-A EUROPEAN JOURNAL

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells

(2013) Aleš Berlec et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Optimal Cleavage and Oxidative Folding of α-Conotoxin TxIB as a Therapeutic Candidate Peptide

(2013) Xiaosa Wu et al.   Marine Drugs

Can too many copies spoil the broth?

(2013) Rochelle Aw et al.   Microbial Cell Factories

Structural and Functional Studies of a Phosphatidic Acid-Binding Antifungal Plant Defensin MtDef4: Identification of an RGFRRR Motif Governing Fungal Cell Entry

(2013) Uma Shankar Sagaram et al.   PLoS One

Strategies for the Production of Recombinant Protein in Escherichia coli

(2013) Gopal Jee Gopal et al.   PROTEIN JOURNAL

Recombinant expression and purification of the tomato defensin TPP3 and its preliminary X-ray crystallographic analysis

(2012) Fung T. Lay et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Enzyme-catalyzed protein crosslinking

(2012) Tobias Heck et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Evaluation of the membrane lipid selectivity of the pea defensin Psd1

(2012) Sónia Gonçalves et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Plant biofarming: Novel insights for peptide expression in heterologous systems

(2012) Antônio Américo Barbosa Viana et al.   BIOPOLYMERS

Identification of defensin-encoding genes of Picea glauca: characterization of PgD5, a conserved spruce defensin with strong antifungal activity

(2012) Pere Picart et al.   BMC PLANT BIOLOGY

Plant Defensins and Defensin-Like Peptides - Biological Activities and Biotechnological Applications

(2012) Andre de Oliveira Carvalho et al.   CURRENT PHARMACEUTICAL DESIGN

A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli

(2012) Hussin A. Rothan et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Dimerization of Plant Defensin NaD1 Enhances Its Antifungal Activity

(2012) Fung T. Lay et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A novel expression system for production of soluble prion proteins in E. coli

(2012) Romany NN Abskharon et al.   Microbial Cell Factories

The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans

(2012) Karin Thevissen et al.   MOLECULAR MICROBIOLOGY

Antimicrobial activity of human β-defensin 4 analogs: Insights into the role of disulfide linkages in modulating activity

(2012) Himanshu Sharma et al.   PEPTIDES

Expression systems for heterologous production of antimicrobial peptides

(2012) Nádia Skorupa Parachin et al.   PEPTIDES

Plant defensins: Common fold, multiple functions

(2012) Nicole L. van der Weerden et al.   Fungal Biology Reviews

Modes of antifungal action and in planta functions of plant defensins and defensin-like peptides

(2012) Barbara De Coninck et al.   Fungal Biology Reviews

Antifungal proteins: More than antimicrobials?

(2012) Nikoletta Hegedüs et al.   Fungal Biology Reviews

Expression, Purification, and Antibacterial Activity of Bovine Lactoferrampin–Lactoferricin in Pichia pastoris

(2011) Xiang-Shan Tang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Lucifensin, a Novel Insect Defensin of Medicinal Maggots: Synthesis and Structural Study

(2011) Václav Čeřovský et al.   CHEMBIOCHEM

Peptide Coupling Reagents, More than a Letter Soup

(2011) Ayman El-Faham et al.   CHEMICAL REVIEWS

Expression of metallocarboxypeptidase inhibitors in Escherichia coli: effect of cysteine content and protein size in the secretory production of disulfide-bridged proteins

(2011) Juan-Miguel Puertas et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

(2011) Van Nguyen et al.   Microbial Cell Factories

Antibiotic activities of host defense peptides: more to it than lipid bilayer perturbation

(2011) Miriam Wilmes et al.   NATURAL PRODUCT REPORTS

Structure-Activity Determinants in Antifungal Plant Defensins MsDef1 and MtDef4 with Different Modes of Action against Fusarium graminearum

(2011) Uma Shankar Sagaram et al.   PLoS One

Portrayal of Complex Dynamic Properties of Sugarcane Defensin 5 by NMR: Multiple Motions Associated with Membrane Interaction

(2011) Viviane Silva de Paula et al.   STRUCTURE

The Antifungal Plant Defensin HsAFP1 from Heuchera Sanguinea Induces Apoptosis in Candida Albicans

(2011) An M. Aerts et al.   Frontiers in Microbiology

Proteins with antifungal properties and other medicinal applications from plants and mushrooms

(2010) Jack H. Wong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant expression, affinity purification and functional characterization of Scots pine defensin 1

(2010) Valentina Kovaleva et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant antimicrobial peptide hPAB-β expressed in Pichia pastoris, a potential agent active against methicillin-resistant Staphylococcus aureus

(2010) Zhijin Chen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Yeast Lipids Can Phase-separate into Micrometer-scale Membrane Domains

(2010) Christian Klose et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Permeabilization of Fungal Hyphae by the Plant Defensin NaD1 Occurs through a Cell Wall-dependent Process

(2010) Nicole L. van der Weerden et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Backbone dynamics of the antifungal Psd1 pea defensin and its correlation with membrane interaction by NMR spectroscopy

(2009) Luciano Neves de Medeiros et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Multifunctional host defense peptides: intracellular-targeting antimicrobial peptides

(2009) Pierre Nicolas   FEBS Journal

The antifungal plant defensin RsAFP2 from radish induces apoptosis in a metacaspase independent way inCandida albicans

(2009) An M. Aerts et al.   FEBS LETTERS

PDC1, a corn defensin peptide expressed in Escherichia coli and Pichia pastoris inhibits growth of Fusarium graminearum

(2009) Pragya Kant et al.   PEPTIDES

Plant defensins—Prospects for the biological functions and biotechnological properties

(2009) André de Oliveira Carvalho et al.   PEPTIDES

In Vitro Activity of the Antifungal Plant Defensin RsAFP2 against Candida Isolates and Its In Vivo Efficacy in Prophylactic Murine Models of Candidiasis

(2008) P. M. Tavares et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Posttransformational vector amplification in the yeastPichia pastoris

(2008) Anthony Jay Sunga et al.   FEMS YEAST RESEARCH

The Plant Defensin, NaD1, Enters the Cytoplasm ofFusarium OxysporumHyphae

(2008) Nicole L. van der Weerden et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Expression and functional characterization of the plant antimicrobial snakin-1 and defensin recombinant proteins

(2008) Natalia Kovalskaya et al.   PROTEIN EXPRESSION AND PURIFICATION

Folding of Conotoxins: Formation of the Native Disulfide Bridges During Chemical Synthesis and Biosynthesis of Conus Peptides

(2007) Grzegorz Bulaj et al.   ANTIOXIDANTS & REDOX SIGNALING