Article
Chemistry, Applied
Rosita Diana, Ugo Caruso, Francesco Silvio Gentile, Luigi Di Costanzo, David Turra, Stefania Vitale, Barbara Panunzi
Summary: In this study, we designed three novel fluorescent brightening agents based on a benzodifuran skeleton, which have improved water solubility and the ability to interact with living cells. A complete understanding of the emission mechanisms was achieved through Density Functional Theory study and X-ray crystallographic analysis. The fluorescence quantum yields were measured in different solvents and at different pH values. Through fluorescence microscopy technique, two probes with different functionalized branching chains were found to be efficient stains for plant root cell wall.
Article
Biochemistry & Molecular Biology
Neus Gacias-Amengual, Lena Wohlschlager, Florian Csarman, Roland Ludwig
Summary: In this study, lignocellulosic enzymes from the white rot fungus Phanerochaete chrysosporium were localized on model substrates and poplar wood using confocal laser scanning microscopy. Two detection approaches were investigated, revealing the innermost secondary cell wall layer as the preferential attack point for cellulose-degrading enzymes. The methods presented are suitable for visualizing enzymes during catalytic biomass degradation and can be further utilized for interaction studies in biorefineries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Mario Costa, Ana Marta Pereira, Silvia Coimbra
Summary: Plant development involves constant adjustments of cell wall composition and structure, with the use of fluorescent immunolocalization offering a high-resolution and specific technique to detect multiple glycan epitopes in the same sample, guiding research and linking glycans to specific functions in plants.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Chemistry, Multidisciplinary
Xiao Liu, Young-Tae Chang
Summary: This article outlines the concepts and mechanisms of different strategies for live cell discrimination and introduces their applications in cell-type discrimination. These strategies include protein-oriented, carbohydrate-oriented, DNA-oriented, gating-oriented, metabolism-oriented, and lipid-oriented approaches. Fluorescent probes can achieve live cell distinction by interacting with biomolecules, accumulating via transporters, and participating in metabolism.
CHEMICAL SOCIETY REVIEWS
(2022)
Review
Biotechnology & Applied Microbiology
Brian T. DeVree, Lisa M. Steiner, Sylwia Glazowska, Felix Ruhnow, Klaus Herburger, Staffan Persson, Jozef Mravec
Summary: Plant cell wall-derived biomass is a renewable source of energy and materials, but its complex structure presents challenges for imaging and understanding. Utilizing molecular probes and light microscopy are important tools to study cell wall assemblies. Advances in imaging techniques and probing methods for plant cell walls can benefit bioengineering efforts and industrial processes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Engineering, Environmental
Jian Zhang, Wei Shen, Cynthia Collings, Kirk A. Vander Meulen, Brian G. Fox, Leida M. Vazquez Ramos, James A. Dumesic, Shi-You Ding
Summary: Thermochemical pretreatment is crucial for processing lignocellulosic biomass to produce biofuels and biomaterials. γ-Valerolactone (GVL) has been used as a green solvent to dissolve lignin effectively. This study compared the performance of HCl and H2SO4 as catalysts in GVL pretreatment, finding that GVL-HCl at 100°C showed similar effectiveness to GVL-H2SO4 at 120°C in terms of xylan and lignin removal. Real-time imaging revealed that GVL-HCl pretreatment at lower temperature could rapidly remove lignin from cell walls and optimize the efficiency of GVL-based pretreatment.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Multidisciplinary Sciences
Satoru Tsugawa, Yuki Yamasaki, Shota Horiguchi, Tianhao Zhang, Takara Muto, Yosuke Nakaso, Kenshiro Ito, Ryu Takebayashi, Kazunori Okano, Eri Akita, Ryohei Yasukuni, Taku Demura, Tetsuro Mimura, Ken'ichi Kawaguchi, Yoichiroh Hosokawa
Summary: This study verifies the formula of elastic shell theory for onion cells using finite element method simulations. The research finds that the tension resulting from turgor pressure regulates cell stiffness, which can be modified by slight adjustments of turgor pressure.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Eric W. J. Gates, Kian Mansour, Sahar Ebrahimi Samani, Sammir Shad, Mari T. Kaartinen, Jeffrey W. Keillor
Summary: Factor XIIIa (FXIIIa) is an important transglutaminase enzyme in the blood coagulation cascade and is a target for the development of anticoagulant drugs. However, current FXIIIa inhibitors lack metabolic stability and have low selectivity over transglutaminase 2 (TG2), preventing clinical evaluation. In this study, we designed and evaluated 21 novel inhibitors targeting FXIIIa, with varying electrophilic warheads, linker lengths, and hydrophobic units to optimize selectivity and potency. We also developed a fluorescent probe, KM93, which selectively labels active FXIIIa with high efficiency and 6.5-fold selectivity over TG2. The structure-activity trends observed with these inhibitors and probe will contribute to future studies on the activity, inhibition, and localization of FXIIIa.
Article
Chemistry, Analytical
Xiao-Yuan Wang, Jian Lv, Qin Hong, Ze-Rui Zhou, Da-Wei Li, Ruo-Can Qian
Summary: This study developed a label-free, noninvasive method for electrochemical single-cell measurement using a quartz nanopipette, which can be used for real-time monitoring of cell membrane rupture under H2O2 treatment. This method allows for precise display of the mechanical status of the cell membrane.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Applied
Xingwei Li, Caixia Yin, Le Zhang, Fangjun Huo
Summary: This review paper discusses recent research progress in the detection of liver cancer-related enzymes (LAP, GGT, and ALP) using fluorescence imaging, which is essential for early diagnosis and treatment of liver cancer. The advantages and disadvantages of various fluorescent probes are compared, and their future development trends are discussed.
Article
Chemistry, Analytical
Yi-Xuan Fu, Wu-Yingzheng Guo, Nan Wang, Yi-Jie Dai, Zi-Ye Zhang, Xin-Lin Sun, Wen-Chao Yang, Guang-Fu Yang
Summary: Bacterial plant diseases pose a serious threat to human food security, causing significant reduction in agricultural yields and economic loss. A new fluorescent sensor targeting Nitroreductase (NTR) was discovered and applied to the imaging of zebrafish and pathogenic bacteria. Importantly, this sensor enabled real-time diagnosis of bacteria-infected Brassica napus L., providing a promising tool for examining temporal and spatial infection of plant pathogens in precision agriculture.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Andreea Veronica Dediu Botezatu, Gabriela Elena Bahrim, Claudia Veronica Ungureanu, Anna Cazanevscaia Busuioc, Bianca Furdui, Rodica Mihaela Dinica
Summary: An efficient one-pot route leading to bis-indolizine symmetric compounds has been developed using a new approach involving biocatalysis. The biocatalysts showed good catalytic potential and led to moderate to excellent yields of the target compounds. Horseradish root was found to be the most promising biocatalyst source, providing higher yields compared to the conventional synthesis method. The structures of the indolizine derivatives were confirmed using various analytical techniques. The obtained compounds exhibited low cytotoxicity on the growth of yeast cells.
GREEN PROCESSING AND SYNTHESIS
(2023)
Article
Chemistry, Analytical
Muling Shi, Xujun Mi, Ling Huang, Liping Qiu, Liu Yang, Xing Sun, Chao Hong, Yingzhuo Yang, Xiaoling Wang, Gao-Qiang Liu
Summary: This study proposes a specific plasma membrane probe and combines it with flow cytometry to establish a rapid, quantitative, and high-throughput method for monitoring cell wall growth. The obtained results are consistent with those of conventional electron microscopy. The proposed probe and approach can be adapted to various applications including the preparation of cell protoplasts, inspection of cell wall integrity under environmental stress, and programmable membrane engineering for cytobiology and physiology research.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Meng Li, Mengqing Yang, Wei-Hong Zhu
Summary: Beta-galactosidase (β-Gal) is a lysosomal hydrolytic enzyme that plays a significant role in the catalytic hydrolysis of glycosidic bonds. Detection of β-Gal activity in vivo using fluorescent sensors has advanced due to their high sensitivity and selectivity. Strategies like aggregation-induced emission (AIE) have been employed to enhance detection accuracy and cellular permeability.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Applied
Rongjuan Sun, Weiji Liu, Timothy V. Kirk, Xiao Dong Chen
Summary: This study aims to investigate the feasibility of using fluorescent probes for bioimaging of enzyme activity in food digestion. The results show that a dual-labeled fluorescent probe can be used to quantitatively measure dextranase activity, and a linear relationship is obtained between the ratio of fluorescence signals and dextranase concentration ratio.
Article
Biochemistry & Molecular Biology
Eleonore Lambert, Veronique Aguie-Beghin, Delphine Dessaint, Laurence Foulon, Brigitte Chabbert, Gabriel Paes, Michael Molinari
Article
Polymer Science
Samir Kasmi, Antoine Gallos, Johnny Beaugrand, Gabriel Paes, Florent Allais
EUROPEAN POLYMER JOURNAL
(2019)
Article
Biotechnology & Applied Microbiology
Gabriel Paes, David Navarro, Yves Benoit, Senta Blanquet, Brigitte Chabbert, Bernard Chaussepied, Pedro M. Coutinho, Sylvie Durand, Igor V. Grigoriev, Mireille Haon, Laurent Heux, Charlene Launay, Antoine Margeot, Yoshiharu Nishiyama, Sana Raouche, Marie-Noelle Rosso, Estelle Bonnin, Jean-Guy Berrin
BIOTECHNOLOGY FOR BIOFUELS
(2019)
Article
Agricultural Engineering
Celine Badouard, Fanny Traon, Clement Denoual, Claire Mayer-Laigle, Gabriel Paes, Alain Bourmaud
INDUSTRIAL CROPS AND PRODUCTS
(2019)
Article
Agricultural Engineering
Aya Zoghlami, Yassin Refahi, Christine Terryn, Gabriel Paes
BIORESOURCE TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Christine Terryn, Anouck Habrant, Gabriel Paes, Corentin Spriet
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Editorial Material
Chemistry, Multidisciplinary
Florent Allais, Xavier Coqueret, Thomas Farmer, Warwick Raverty, Caroline Remond, Gabriel Paes
FRONTIERS IN CHEMISTRY
(2020)
Review
Chemistry, Multidisciplinary
Aya Zoghlami, Gabriel Paes
FRONTIERS IN CHEMISTRY
(2019)
Review
Biotechnology & Applied Microbiology
Claire Bourlieu, Thierry Astruc, Sophie Barbe, Jean-Guy Berrin, Estelle Bonnin, Rachel Boutrou, Virginie Hugouvieux, Steven Le Feunteun, Gabriel Paes
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biotechnology & Applied Microbiology
Amandine Leroy, Xavier Falourd, Loic Foucat, Valerie Mechin, Fabienne Guillon, Gabriel Paes
Summary: Hot water pretreatment (HWP) alters the composition and structure of biomass, increasing saccharification kinetics and yield. Changes in cell wall composition, lignin structure, and cellulose environment after HWP contribute to enhanced accessibility of cellulases and promote hydrolysis. Understanding the interactions between polymers is crucial for optimizing HWP in sustainable biorefineries.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Claire Mayer-Laigle, Laurence Foulon, Clement Denoual, Miguel Pernes, Eric Rondet, Anthony Magueresse, Cecile Barron, Anouck Habrant, Alain Bourmaud, Gabriel Paes
Summary: The study successfully prepared reinforced filaments containing fluorescent flax particles through extrusion and 3D printing. Microstructural characterization showed that the fluorescent flax particles were homogeneously distributed in the 3D printed material. Despite the low content, a strong fluorescent emission could still be measured.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Chemistry, Multidisciplinary
Sacha Escamez, Christine Terryn, Madhavi Latha Gandla, Zakiya Yassin, Gerhard Scheepers, Torgny Nasholm, Ola Sundman, Leif J. Jonsson, Judith Lundberg-Felten, Hannele Tuominen, Totte Niittyla, Gabriel Paes
Summary: This study demonstrates the relationship between fluorescence lifetime and chemical composition of lignin, a major structural element in plant cell walls. By using FLIM and classical analytical methods, researchers accurately predicted the content of different lignin units in hybrid aspen trees with varying cell wall chemistry and structure. These findings provide insights into lignin biosynthesis dynamics and potential improvements for woody biomass feedstocks.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Agricultural Engineering
Amandine Leroy, Marie-Francoise Devaux, Mathieu Fanuel, Hugo Chauvet, Sylvie Durand, Camille Alvarado, Anouck Habrant, Christophe Sandt, Helene Rogniaux, Gabriel Paes, Fabienne Guillon
Summary: This work provides a dynamic perspective on the enzymatic degradation of maize cell walls and sheds new light on the recalcitrance of hot water pretreated maize stem internodes. Depending on their polymer composition and organization, different cell types exhibit varying degrees and rates of enzymatic degradation. Enzymes sequentially act from cell walls rich in accessible cellulose to the most recalcitrant cells, and this phenomenon may be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with cell type.
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Julien du Pasquier, Gabriel Paes, Patrick Perre
Summary: This review critically analyzed the current state of dilute acid pretreatment applied to lignocellulosic biomass. Data from 63 publications were analyzed, and it was found that most studies used residence times of less than 30 minutes, temperature ranges from 100°C to 200°C, and acid levels between 0% and 2%. Yields were quantified by measuring xylose content or glucose content after enzymatic hydrolysis. Statistical analyses were performed to determine the time-temperature equivalence for three types of biomass, and non-linear expressions were used for formulation. Further research is recommended to explore less investigated areas, such as moderate temperature levels with longer residence times. Standardization and better description of pretreatment materials (time-temperature kinetics, reactor type) and analytical methods are necessary. The development of an open, findable, accessible, interoperable, and reusable data approach for pretreatment research is mandatory.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Edwige Audibert, Berangere Lebas, Corentin Spriet, Anouck Habrant, Brigitte Chabbert, Gabriel Paes
Summary: This study proposes an automated method for the quantification of chemical and morphological parameters through fluorescence macroscopy, which was applied on wood samples pretreated with steam explosion. The results show that steam explosion has a significant impact on the wood samples, altering the fluorescence intensity and causing morphological changes such as cell shrinkage and deformation of cell walls. The developed procedure allows simultaneous and effective quantification of morphological parameters and fluorescence intensity of the cell walls, providing valuable insights into biomass architecture.
