期刊
MOLECULES
卷 19, 期 9, 页码 13319-13331出版社
MDPI
DOI: 10.3390/molecules190913319
关键词
small interfering RNA (siRNA) delivery; gold nanoparticles; cyclic peptides; siRNA delivery systems (DDS)
资金
- National Center for Research Resources, NIH [8 P20 GM103430-12]
Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR](5) and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR](5) and [WR](5)-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR](5) and [WR](5)-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR](5) and [WR](5)-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据