4.6 Article

Atomic Model and Micelle Dynamics of QS-21 Saponin

期刊

MOLECULES
卷 19, 期 3, 页码 3744-3760

出版社

MDPI
DOI: 10.3390/molecules19033744

关键词

QS-21; saponins; glycoconjugates; molecular dynamics; SAXS; micelle; adjuvant

资金

  1. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
  2. Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
  3. Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS)
  4. Ministry of Science and Innovation of Spain [RYC-2005-001625, MAT 2008-01080/MAT]
  5. Centro Nacional de Supercomputacao of the Universidade Federal do Rio Grande do Sul (CESUP/UFRGS)
  6. MCT
  7. MEC, Brasilia
  8. DF, Brazil

向作者/读者索取更多资源

QS-21 is a saponin extracted from Quillaja saponaria, widely investigated as a vaccine immunoadjuvant. However, QS-21 use is mainly limited by its chemical instability, significant variety in molecular composition and low tolerance dose in mammals. Also, this compound tends to form micelles in a concentration-dependent manner. Here, we aimed to characterize its conformation and the process of micelle formation, both experimentally and computationally. Therefore, molecular dynamics (MD) simulations were performed in systems containing different numbers of QS-21 molecules in aqueous solution, in order to evaluate the spontaneous micelle formation. The applied methodology allowed the generation of micelles whose sizes were shown to be in high agreement with small-angle X-ray scattering (SAXS). Furthermore, the ester linkage between fucose and acyl chain was less solvated in the micellar form, suggesting a reduction in hydrolysis. This is the first atomistic interpretation of previous experimental data, the first micellar characterization of saponin micelles by SAXS and first tridimensional model of a micelle constituted of saponins, contributing to the understanding of the molecular basis of these compounds.

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