期刊
MOLECULES
卷 17, 期 6, 页码 7241-7254出版社
MDPI
DOI: 10.3390/molecules17067241
关键词
DNA damage; antioxidant; comet assay; red algae; cell cycle arrest
资金
- Ministry of Education of ROC under marine technology research and development center [98D1024]
- Kaohsiung Medical University Research Foundation
- Department of Health, Executive Yuan, R.O.C. (TAIWAN) [DOH101-TD-C-111-002]
- Kaohsiung Medical University Research Foundation [KMUER014, KMU-M110001]
- NSYSU-KMU JOINT RESEARCH PROJECT [NSYSUKMU 101-006]
Potential antioxidant properties of an aqueous extract of the edible red seaweed Gracilaria tenuistipitata (AEGT) against oxidative DNA damage were evaluated. The AEGT revealed several antioxidant molecules, including phenolics, flavonoids and ascorbic acid. In a cell-free assay, the extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity that significantly reduced H2O2-induced plasmid DNA breaks in a dose-response manner (P < 0.001). The AEGT also suppressed H2O2-induced oxidative DNA damage in H1299 cells by reducing the percentage of damaged DNA in a dose-response manner (P < 0.001) as measured by a modified alkaline comet-nuclear extract (comet-NE) assay. The MTT assay results showed that AEGT confers significant protection against H2O2-induced cytotoxicity and that AEGT itself is not cytotoxic (P < 0.001). Moreover, H2O2-induced cell cycle G2/M arrest was significantly released when cells were co-treated with different concentrations of AEGT (P < 0.001). Taken together, these findings suggest that edible red algae Gracilaria water extract can prevent H2O2-induced oxidative DNA damage and its related cellular responses.
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