期刊
MOLECULES
卷 16, 期 3, 页码 2583-2598出版社
MDPI
DOI: 10.3390/molecules16032583
关键词
Panduratin A; apoptosis; High Content Screening; Real-time Cellular Analyzer; NF-kappa B
资金
- University of Malaya [UMRG RG004/09BIO]
In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 mu g/mL (10.8 mu M). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-kappa B) translocation from cytoplasm to nuclei activated by tumor necrosis factor-alpha (TNF-alpha), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line, suggesting its therapeutic potential as an NF-kappa B inhibitor.
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