Article
Chemistry, Physical
Jing Liu, Xuefei Yin, Zitao Li, Xiaocong Wu, Zhaoxuan Zheng, Junqiang Fang, Guofeng Gu, Peng G. Wang, Xianwei Liu
Summary: In this study, a highly efficient enzymatic approach was developed for the regioselective construction of diverse 1,2-cis beta-mannosyl linkages using readily available recombinant glycoside phosphorylases. This method enabled the synthesis of a variety of naturally occurring beta-mannosides containing different beta-mannosyl linkages without the need for expensive nucleotide-activated sugar donors.
Article
Chemistry, Multidisciplinary
Jonathan P. Dolan, Sebastian C. Cosgrove, Gavin J. Miller
Summary: Despite the significant progress in the field of biocatalysis, specifically in carbohydrate-active enzymes and sugar nucleotides involved in glycan biosynthesis, further research is needed to understand substrate promiscuity and utilize biocatalysis fundamentals for expanding substrate scopes and improving enzyme stability and kinetics. Additionally, the use of biocatalysis to obtain stereochemically defined carbohydrate materials that can undergo late-stage chemical functionalization or automated glycan synthesis/polymerization is becoming increasingly important.
Article
Biochemistry & Molecular Biology
Zijia Lu, Carl Ramgard, Irem Ergenlioglu, Lova Sandin, Hugo Hammar, Helena Andersson, Katharine King, Annie R. Inman, Mengshu Hao, Vincent Bulone, Lauren S. McKee
Summary: The genome of soil bacterium Bacteroidota Chitinophaga pinensis contains a large number of glycoside hydrolases (GHs) with unique features that could have novel functions. These GHs show potential activity on polysaccharides found in fungal and oomycete cell walls, including chitin, beta-1,3-glucan, and beta-1,6-glucan. This study characterizes the ability of C. pinensis to hydrolyze beta-1,6-glucan and identifies three enzymes encoded by its genome that exhibit varying levels of beta-1,6-glucanase activity. These findings have implications for biocontrol of pathogens and the potential use of these enzymes in biomedical research and drug synthesis.
Review
Chemistry, Organic
Riccardo Semproli, Marina S. Robescu, Mattia Cambo, Klodiana Mema, Teodora Bavaro, Marco Rabuffetti, Daniela Ubiali, Giovanna Speranza
Summary: Esters of sn-glycero-3-phosphoric acid are derivatives of glycerophospholipids where both fatty acid chains at sn-1 and sn-2 positions are removed. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine are the most abundant glycerophospholipids in Nature. Their deacylated derivatives have applications in various industrial sectors, with examples like sn-glycero-3-phosphocholine as a cognitive enhancer and food ingredient, and sn-glycero-3-phosphoinositol as a natural anti-inflammatory agent.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Franciela Arenhart Soares, Alexander Steinbuchel
Summary: Diene rubbers are polymeric materials with double bonds and elastic properties, which can undergo post-polymerization modifications through enzymatic or chemical methods. Enzymes serve as environmentally friendly catalysts, and biodegradation and biomodifications have become popular research topics. Chemical modifications using click-chemistry are important for creating new materials, while combining enzymatic and chemical methods can lead to effective modifications in diene rubbers.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Microbiology
Hexue Jia, Xiaoting Feng, Jiamin Huang, Yingjie Guo, Daolei Zhang, Xuezhi Li, Jian Zhao
Summary: This study showed that CBM1s can enhance the saccharification of lignocellulose as an accessory protein in cellulase cocktails. The promoting effects of CBM1s are related to their sources, dosages, and substrate characteristics.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Organic
Yu Nakagawa, Yuzuka Oya, Makoto Ojika, Yasuhiro Igarashi, Yukishige Ito
Summary: This study discovered that the modified derivatives of C4'-N-formyl PRM-A and C4'-N-acetyl PRM-A showed significant antifungal activities without forming aggregates or precipitates, providing a new strategy for the therapeutic application of PRM-A.
Article
Agronomy
Shubranil Das, Manoj Kundu, Samik Sengupta, Sareeta Nahakpam
Summary: Most commercial mango cultivars have the tendency of alternate bearing, but little is known about the physiological mechanism behind flowering during on and off years. This study aimed to understand the physio-chemical and nutritional basis for on- and off-season bearing in different commercial mango cultivars. The results showed that the physio-chemical attributes in the bud at bud break stage were directly related to panicle emergence in mango, while increased macronutrients and higher activities of oxidative enzymes led to off-season bearing.
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Wei Cao, Risa Watanabe, Yoshitaka Ishii, Kohtaro Kirimura
Summary: This study presents a simple enzymatic reaction for the selective production of alpha-AGs using the glucosyl transfer enzyme XgtA. The addition of glucose isomerase improves the yield of alpha-OG.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Oncology
Rafat M. Mohareb, Maher H. E. Helal, Sara S. Mohamed, Amira E. M. Abdallah
Summary: Novel heterocyclic compounds with high impact on biological activities were synthesized and evaluated for their anti-cancer cell line activity and enzyme inhibition. The results demonstrated significant anti-proliferative and inhibitory effects of these compounds.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Irina O. Romanchuk, Tetyana Rudakova, Antonina Minoroya, Liudmyla O. Moiseeva
Summary: This study aimed to explore innovative approaches for determining carbohydrate content in dairy products, particularly focusing on fructose. The resorcinol method was used to determine the fructose content, and the reliability of the results was verified through precipitation of the product solution using the polarimetric method for lactose degradation.
JOURNAL OF CHEMISTRY AND TECHNOLOGIES
(2022)
Article
Chemistry, Organic
Mitra Rezanezhad, Kobra Nikoofar, Fatemeh Molaei Yielzoleh
Summary: In this research, two novel nano-size inorganic-bioorganic hybrids based on graphene oxide were prepared. The bio-based ionic liquids obtained through the reaction of arginine and malonic acid were characterized. These bio-based ionic liquids were then embedded on graphene oxide core to form the final nano-size inorganic-bioorganic hybrids. The catalytic efficacy of the obtained nanostructures in the synthesis of various compounds was examined, and it was found that one of the nanostructures exhibited faster catalytic reactions than the other. The recovery and reusability of the nanostructure with higher catalytic efficacy were successfully demonstrated.
POLYCYCLIC AROMATIC COMPOUNDS
(2023)