期刊
MOLECULAR THERAPY
卷 26, 期 12, 页码 2751-2765出版社
CELL PRESS
DOI: 10.1016/j.ymthe.2018.09.006
关键词
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资金
- National Natural Science Foundation of China [81472202, 81772932, 81201535, 81302065, 81702243, 81472209, 81301993]
- Fundamental Research Funds for the Central Universities [22120170212, 22120170117]
- Shanghai Natural Science Foundation [12ZR1436000, 16ZR1428900]
- Shanghai Municipal Commission of Health and Family Planning [201540228, 201440398]
- Jilin Provincial Science and Technology Department [20140414061GH]
Increasing evidence indicates that tumor-initiating cells (TICs) are responsible for the occurrence, development, recurrence, and development of the drug resistance of cancer. MicroRNA (miRNA) plays a significant functional role by directly regulating targets of TIC-triggered non-small-cell lung cancer (NSCLC), but little is known about the function of the miR-30 family in TICs. In this study, we found the miR-30 family to be downregulated during the spheroid formation of NSCLC cells, and patients with lower miR-30a/c expression had shorter overall survival (OS) and progression-free survival (PFS). Moreover, transmembrane 4 super family member 1 (TM4SF1) was confirmed to be a direct target of miR-30a/c. Concomitant low expression of miR-30a/c and high expression of TM4SF1 correlated with a shorter median OS and PFS in NSCLC patients. miR-30a/c significantly inhibited stem-like characteristics in vitro and in vivo via suppression of its target gene TM4SF1, and then it inhibited the activity of the mTOR/AKT-signaling pathway. Thus, our data provide the first evidence that TM4SF1 is a direct target of miR-30a/c and miR-30a/c inhibits the stemness and proliferation of NSCLC cells by targeting TM4SF1, suggesting that miR-30a/c and TM4SF1 may be useful as tumor biomarkers for the diagnosis and treatment of NSCLC patients.
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