Article
Nanoscience & Nanotechnology
Xingjie Hu, Yan Huang, Hong Zheng, Jiahui Liu, Mengmeng Liu, Mo Xie, Chunhai Fan, Nan Chen
Summary: Framework nucleic acids (FNAs) are a new type of DNA-based nanomaterials with potential applications in biosensing, bioimaging, and molecular delivery. Hierarchical DNA nanostructures composed of multiple FNAs increase drug delivery capacity and multifunctional modification. However, there are limited studies on the behavior and regulation of hierarchical FNAs in living cells, hindering their further applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Medicinal
Farid R. El-Gamal, Mohamed A. Akl, Hammam A. Mowafy, Hidefumi Mukai, Shigeru Kawakami, Mohsen Afouna
Summary: This study developed novel octaarginine (R-8) peptide-HFQ lipid grafted PEGylated liposomes for glioma cells targeting. The results showed that these liposomes exhibited excellent in vitro cellular association and intracellular distribution properties, suggesting their potential as a drug delivery system for tumor targeting.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Shirleide Santos Nunes, Juliana de Oliveira Silva, Renata Salgado Fernandes, Sued Eustaquio Mendes Miranda, Elaine Amaral Leite, Marcelo Alexandre de Farias, Rodrigo Villares Portugal, Geovanni Dantas Cassali, Danyelle M. Townsend, Monica Cristina Oliveira, Andre Luis Branco de Barros
Summary: PEGylated liposomes are widely studied as drug delivery systems, but their interaction with cells may be hindered by the addition of PEG. This study compared the characteristics and drug delivery capabilities of PEGylated and non-PEGylated liposomes. The results showed that non-PEGylated liposomes had better cellular uptake and were more effective in controlling tumor growth.
Article
Chemistry, Analytical
Kaisa Rautaniemi, Thomas John, Maximilian Richter, Benedikt C. Huck, Jacopo Zini, Brigitta Loretz, Claus -Michael Lehr, Elina Vuorimaa-Laukkanen, Ekaterina Lisitsyna, Timo Laaksonen
Summary: The analysis of nanoparticle dynamics in live cells using video tracking can provide detailed information on their interactions and trafficking. In this study, we compared the trajectories, diffusion coefficients, and particle velocities of different nanoparticles in cells using a simple experimental setup. We found similar trajectory patterns for all nanoparticles, resembling intracellular vesicular transport, but the cell-derived extracellular vesicles exhibited faster movement, possibly due to their content and motor protein involvement.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Sylvie Egloff, Anne Runser, Andrey Klymchenko, Andreas Reisch
Summary: This study investigates the influence of nanoparticle (NPs) size on their delivery into the cytosol via electroporation, finding that NPs smaller than 20 nm can be efficiently delivered while only particles with a core size smaller than 15 nm can freely diffuse in the cytosol. The results provide clear methodological and design guidelines for the safe delivery of NPs for intracellular applications.
Article
Pharmacology & Pharmacy
Yuri Sugimoto, Tadaharu Suga, Mizuki Umino, Asako Yamayoshi, Hidefumi Mukai, Shigeru Kawakami
Summary: Functionalized drug delivery systems have been developed to improve the targeted delivery and intracellular transportation of therapeutic drugs. In this study, we focused on the quality of functional lipids to prepare targeted nanoparticles using microfluidic devices. We found that KK-(EK)(4)-lipid, a lipid with near-neutral charge, enhances the cellular association and intracellular translocation of nanoparticles. We also demonstrated the potential of KK-(EK)(4)-lipid in enhancing the cellular association and protein expression of extracellular vesicles and mRNA-LNPs.
Article
Multidisciplinary Sciences
Chao Jiang, Hong-Yu Luo, Xinpeng Xu, Shuo-Xing Dou, Wei Li, Dongshi Guan, Fangfu Ye, Xiaosong Chen, Ming Guo, Peng-Ye Wang, Hui Li
Summary: Cell migration plays important roles in biological processes, but the mechanisms behind how cells regulate their speed and direction are still unclear. This study reveals a strong positive correlation between intracellular diffusion and cell migration speed, and identifies a switching of cell migration modes associated with reversible intracellular diffusion variation and lamellipodium structure deformation. The findings suggest a mechanism involving actin polymerization and molecular crowding in the lamellipodium to coordinate intracellular diffusion dynamics and lamellipodium structure during cell migration.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Matteo Bernardello, Radoslaw J. Gora, Patrick Van Hage, Gustavo Castro-olvera, Emilio J. Gualda, Marcel J. M. Schaaf, Pablo Loza-alvarez
Summary: This study introduces a light-sheet fluorescence microscopy setup for imaging individual proteins inside living zebrafish embryos, allowing for the analysis of protein dynamics. The optical configuration of this design is accessible to many laboratories, and a dedicated sample-mounting system ensures sample viability and flexibility in mounting.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Computer Science, Information Systems
Yuqi Xiao, Yongjun Wu, Fan Yang
Summary: This paper proposes an advanced particle filter (PF) algorithm based on the quantum particle swarm optimization method (QPSO) and adaptive genetic algorithm (AGA). The PF is improved by applying the position updating equation of QPSO and replacing individuals with lower fitness. The accuracy and sample diversity are increased by applying the genetic operation from AGA. Furthermore, a frame size adaptive adjustment model is proposed to improve target positioning accuracy.
MULTIMEDIA TOOLS AND APPLICATIONS
(2023)
Article
Plant Sciences
Jingjing Xing, Dongchao Ji, Zhikun Duan, Tong Chen, Xiaomin Luo
Summary: The plant receptor-like kinase FERONIA (FER) plays multiple roles in regulating cellular processes such as cell growth, hormone signaling and response to pathogens. This study found that flg22 stimulus promotes the dissociation of FER from the plasma membrane and its localization to endosomes. FER undergoes constitutive endocytosis and recycling, and is essential for flg22 perception and immune activation.
Article
Chemistry, Analytical
Amir Hatamie, Lin Ren, Xinwei Zhang, Andrew G. Ewing
Summary: The presence of carbon nanotubes on electrodes significantly increases the number of catecholamines in vesicles and enhances current intensity, indicating strong interaction between carbon nanotubes and vesicles.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Nidhi Malviya, A. Prabakaran, Amit Alexander
Summary: The present study aimed to improve the permeability of sinapic acid (SA) across the skin by preparing vesicular nanoformulations such as transferosomes and ethosomes. The results showed that ethosomes exhibited higher permeability compared to transferosomes and plain SA hydrogel, which was attributed to their flexibility across the skin.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Pharmacology & Pharmacy
Clara Colino, David Velez Gomez, Estela Alonso Horcajo, Carmen Gutierrez-Millan
Summary: This study developed lipidic nanoformulations of quercetin for dermal use. The formulated liposomes and chitosomes showed suitable characteristics for dermal administration and preserved the antioxidant activity of quercetin.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Shradha Dattani, Xiaoling Li, Charina Lampa, David Lechuga-Ballesteros, Amanda Barriscale, Behzad Damadzadeh, Bhaskara R. Jasti
Summary: The purpose of this study was to compare LDV-targeted lipid-based micelles, liposomes, and solid lipid nanoparticles (SLN) in vitro and in vivo to investigate their therapeutic potential. The nanocarriers were characterized for their physicochemical properties, drug loading efficiency, release behavior, stability, and antitumor efficacy in a melanoma xenograft model. Results showed that SLN had the slowest drug release rate and highest stability, and demonstrated a statistically significant delay in tumor growth compared to the other two formulations. This study highlights the potential of SLN as drug delivery vehicles for anticancer therapy.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Automation & Control Systems
Daniel K. D. Villa, Alexandre S. Brandao, Mario Sarcinelli-Filho
Summary: This paper presents a complete control architecture for implementing adaptive sliding mode controllers in quadrotors. It compares three common methods for implementing these controllers and concludes that one of the adaptive sliding mode controllers performs exceptionally well, outperforming the PID controller.
JOURNAL OF THE FRANKLIN INSTITUTE-ENGINEERING AND APPLIED MATHEMATICS
(2023)
Article
Immunology
Tanjila Hasan, Ryo Kawanishi, Hidetaka Akita, Yoshifumi Nishikawa
Summary: ssPalmE-LNP is an effective carrier for DNA vaccines against protozoan infections, leading to increased survival rates and antibody production in mice. Further immunological studies are needed to explore the mechanism of protective immunity induced by ssPalmE-LNPTgGRA15.
Article
Pharmacology & Pharmacy
Naoki Cho, Hiroshi Suemizu, Hidetaka Kamimura, Tomoyuki Ohe, Fumi Ito, Hidetaka Akita, Kaoru Kobayashi
Summary: This study used a humanized-liver mouse model to investigate the pharmacokinetic profiles of benzbromarone and the generation of reactive metabolites. The results showed that the metabolic profiles of benzbromarone in humanized-liver mice were similar to those in humans, and the detected reactive metabolites were associated with benzbromarone-induced hepatotoxicity.
DRUG METABOLISM AND PHARMACOKINETICS
(2022)
Article
Medicine, Research & Experimental
Keita Kitamura, Ayaka Okamoto, Hanae Morio, Ryuto Isogai, Ryo Ito, Yoshiyuki Yamaura, Saki Izumi, Takafumi Komori, Shingo Ito, Sumio Ohtsuki, Hidetaka Akita, Tomomi Furihata
Summary: This study characterizes the ability of the hiMCS-BBB models to evaluate receptor-mediated transcytosis (RMT)-mediated blood-brain barrier (BBB) penetration properties of middle/macromolecules. The results show that the hiMCS-BBB models have the potential to provide valuable tools for exploring and identifying middle/macromolecules with excellent BBB permeability levels.
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Hiroki Tanaka, Shinya Hagiwara, Daiki Shirane, Takuma Yamakawa, Yuka Sato, Chika Matsumoto, Kota Ishizaki, Miho Hishinuma, Katsuyuki Chida, Kasumi Sasaki, Etsuo Yonemochi, Keisuke Ueda, Kenjirou Higashi, Kunikazu Moribe, Takashi Tadokoro, Katsumi Maenaka, Sakura Taneichi, Yuta Nakai, Kota Tange, Yu Sakurai, Hidetaka Akita
Summary: There is a growing demand by researchers to test whether their own biological findings might be applicable for use in mRNA-based therapeutics, but the equipment and know-how required for manufacturing such nanoparticles is often inaccessible. A simple method for preparing mRNA-LNPs is prerequisite to encourage more innovation in mRNA therapeutics. This study reports on a method for encapsulating IVT-mRNA into LNPs by rehydrating a Ready-to-Use empty freeze-dried LNP formulation with IVT-mRNA solution followed by heating, and the resulting mRNA-LNPs had a similar structure compared to the mRNA-LNPs prepared by conventional microfluidic mixing. In vivo genome editing using these mRNA-LNPs was achieved.
Article
Chemistry, Multidisciplinary
Yu Sakurai, Keito Yoshikawa, Kenta Arai, Akira Kazaoka, Shigeki Aoki, Kousei Ito, Yuta Nakai, Kota Tange, Tomomi Furihata, Hiroki Tanaka, Hidetaka Akita
Summary: This study targets lymphatic endothelial cells (LEC) using ionizable lipid-formulated nanoparticles (LNPs) complexed with Apolipoprotein E (ApoE). The LNPs are taken up by LECs in an ApoE-dependent manner, as shown in in vitro studies. Modifying the surface of the LNPs with a high density of PEG-conjugated lipids with short acyl chains enables effective targeting of LECs after subcutaneous administration. This approach significantly inhibits the expression of the LEC marker VEGFR3.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Mizuki Doi, Hiroki Tanaka, Takara Ohoto, Naoya Miura, Yu Sakurai, Hiroto Hatakeyama, Hidetaka Akita
Summary: The reactivation of anticancer immunity using immune checkpoint inhibitors (ICIs) is a crucial aspect in cancer immunotherapy. However, the low objective response rate (<40%) of ICIs remains a major challenge. This study demonstrates that myeloid-derived suppressor cells (MDSCs) can be depleted by delivering synthetic glucocorticoid dexamethasone to phagocytic cells in the spleen using lipid nanoparticles. Additionally, the nanoparticulated dexamethasone exhibits a synergistic anticancer effect with ICIs.
Article
Engineering, Biomedical
Masaki Gomi, Yu Sakurai, Minami Sato, Hiroki Tanaka, Yumi Miyatake, Koichi Fujiwara, Mizuki Watanabe, Satoshi Shuto, Yuta Nakai, Kota Tange, Hiroto Hatakeyama, Hidetaka Akita
Summary: This study reports the development of a new lipid nanoparticle (LNP) that efficiently delivers messenger RNA (mRNA) to secondary lymphoid tissues. A microfluidic technique was used to incorporate alcohol-soluble phosphatidylserine (PS) derivatives, designed to target immune cells, into LNPs with high scalability and reproducibility. The resulting PS-loaded LNP delivered mRNA to the spleen more efficiently than a control LNP. Sub-organ analysis revealed extensive uptake of the PS-loaded LNP by tissue-resident macrophages in the red pulp and marginal zone of the spleen. Therefore, the PS-loaded LNP described in this study holds promise for clinical applications involving mRNA delivery to the spleen.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Analytical
Menglu Li, Yukiko Ueyama-Toba, Matthew Lindley, Gunganist Kongklad, Yasunori Nawa, Yasuaki Kumamoto, Seiichi Ishida, Yasunari Kanda, Satoshi Fujita, Hiroyuki Mizuguchi, Katsumasa Fujita
Summary: To promote the clinical application of hiPSC-derived hepatocytes, a label-free Raman microscopy method is used to monitor regenerative processes and assess differentiation efficiency. The method has successfully identified intracellular biomolecules in live samples and compared the phenotypes of hiPSC-derived hepatocytes with HepaRG and commercially available iCell hepatocytes. The study also explored the use of Raman imaging as a hepatotoxicity assay, demonstrating its potential for quality control and hepatotoxicity screening.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jumpei Inui, Yukiko Ueyama-Toba, Seiji Mitani, Hiroyuki Mizuguchi
Summary: A method has been developed to dissociate, cryopreserve, and reseed human induced pluripotent stem cell-derived hepatocyte-like cells (HLCs) without loss of their functions. This technology enables the availability of cryopreserved HLCs for drug discovery research.
Article
Biology
Eiko Sakai, Tsutomu Imaizumi, Ruruka Suzuki, Marcos Taracena-Gandara, Toshiki Fujimoto, Fuminori Sakurai, Hiroyuki Mizuguchi
Summary: Non-alcoholic liver disease (NAFLD) is caused by excessive fat accumulation in the liver and miR-27b has been found to play a crucial role in NAFLD development by targeting genes involved in lipid metabolism. This study identified MAIP1 as a direct target of miR-27b and showed that overexpression of MAIP1 can ameliorate lipid accumulation induced by miR-27b. Additionally, decreased Maip1 expression was observed in mice fed a high-fat diet, suggesting its involvement in fatty liver condition and its potential as a therapeutic target for NAFLD.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sumiyo Morita, Takuro Horii, Mika Kimura, Ryosuke Kobayashi, Hiroki Tanaka, Hidetaka Akita, Izuho Hatada
Summary: Knockout mice are important tools for studying the normal function of genes. Liver-specific conditional knockout mice can be generated using lipid nanoparticles, which offers a more convenient method compared to traditional approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Daiki Shirane, Hiroki Tanaka, Yu Sakurai, Sakura Taneichi, Yuta Nakai, Kota Tange, Itsuko Ishii, Hidetaka Akita
Summary: The lipid nanoparticle (LNP) is a promising nanotechnology for delivering RNA molecules, including siRNA and mRNA. LNPs encoding the antigen protein of SARS-CoV-2 have been approved as mRNA vaccines against the disease. LNP formulations are generally metastable, and their properties can shift towards a more stable state during long-term storage. To enable easier storage, a one-pot method was used to prepare freeze-dried formulations of mRNA-LNPs that can be stored at 4°C for at least 4 months.
Article
Pharmacology & Pharmacy
Xuan Du, Erica Yada, Yuki Terai, Takuya Takahashi, Hideyuki Nakanishi, Hiroki Tanaka, Hidetaka Akita, Keiji Itaka
Summary: This study compared the performance of nanomicelles and LNPs in mRNA delivery. The nanomicelles showed relatively sustained protein production and reduced inflammation, making them suitable for therapeutic purposes, while LNPs exhibited rapid mRNA expression and a potent immune response, making them suitable for vaccine purposes. These results provide support for further optimization of mRNA delivery systems tailored for specific purposes.
Article
Chemistry, Medicinal
Masaki Gomi, Yuka Nakayama, Yu Sakurai, Ryotaro Oyama, Koki Iwasaki, Mizuki Doi, Yi Liu, Mizuho Hori, Himeka Watanabe, Kohei Hashimoto, Hiroki Tanaka, Kota Tange, Yuta Nakai, Hidetaka Akita
Summary: This study investigates the delivery of mRNA encoding autoantigens to the spleen using lipid nanoparticles (LNPs) containing phosphatidylserine (PS). The expressed MOG35-55 antigens induce immune tolerance and could potentially be used as a treatment for experimental autoimmune encephalitis (EAE).
Review
Chemistry, Organic
Hiroki Tanaka, Hidetaka Akita
Summary: Specialized medicine is now expanding from low-molecular drugs to recombinant proteins, antibodies, and nucleic acids. Among them, RNA therapy is growing worldwide as an alternative gene complement approach. The use of short nucleic acids such as siRNA and miRNA to regulate disease-related genes shows promising results in the field of medicine.
JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN
(2022)
