期刊
MOLECULAR THERAPY
卷 17, 期 5, 页码 880-888出版社
CELL PRESS
DOI: 10.1038/mt.2009.34
关键词
-
资金
- Leukemia and Lymphoma Society Specialized Center of Research [7018]
- NIH [P50CA126752, U54 HL081007, RO1CA131027]
- Leukemia and Lymphoma Society Translational Research
- Doris Duke Charitable Foundation/Clinical Scientist development
- Everyone Survives (WES) foundation
Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) can induce objective clinical responses in patients with malignant diseases. The option of providing a proliferative and survival advantage to adoptively transferred CTLs remains a challenge to improve their efficacy. Host lymphodepletion and administration of recombinant interleukin-2 (IL-2) are currently used to improve CTL survival and expansion after adoptive transfer, but these approaches are frequently associated with significant side effects and may increase proliferation of T regulatory cells. IL-7 is a crucial homeostatic cytokine that has been safely administered as a recombinant protein. However, while IL-7 induces robust expansion of naive and memory T lymphocytes, the lack of expression of the IL-7 receptor alpha chain (IL-7R alpha) by CTLs precludes their response to this cytokine. We found that CTLs can be genetically modified to re-express IL-7R alpha, and that this manipulation restores the response of these cells to IL-7 without apparent modification of their antigen specificity or dependency, and without changing their response to other common gamma (gamma c) chain cytokines. This approach may allow selective expansion of CTLs without the unwanted effects associated with IL-2.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据