Article
Biotechnology & Applied Microbiology
Min J. Jang, Gerard M. Coughlin, Cameron R. Jackson, Xinhong Chen, Miguel R. Chuapoco, Julia L. Vendemiatti, Alexander Z. Wang, Viviana Gradinaru
Summary: In this study, a ultrasensitive, sequential fluorescence in situ hybridization (USeqFISH) method was developed for spatial transcriptomic profiling of endogenous and viral RNA. The method allows investigation of the transduction and cell subtype biases of different AAV variants, as well as profiling of pooled regulatory cargos in AAV genomes. USeqFISH also shows potential applications in in situ AAV profiling and multimodal single-cell analysis in non-human primates.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rebecca K. Dirkes, Nathan C. Winn, Thomas J. Jurrissen, Dennis B. Lubahn, Victoria J. Vieira-Potter, Jaume Padilla, Pamela S. Hinton
Summary: The study found that in male mice, ERKO has a positive impact on bone density, with exercise having little effect. ERKO has a negative impact on cortical geometry, but exercise can partially reverse this effect. Neither ERKO nor exercise have an effect on sclerostin expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geriatrics & Gerontology
Nina E. Baumgartner, Katelyn L. Black, Shannon M. McQuillen, Jill M. Daniel
Summary: Both previous and continuous midlife estradiol treatment can increase levels of nuclear ER alpha in the hippocampus, impacting gene expression and protein levels related to estrogen effects on memory, indicating a long-lasting regulatory role of hippocampal ER alpha in memory.
NEUROBIOLOGY OF AGING
(2021)
Article
Biochemistry & Molecular Biology
Xiaoyu Song, Wanfu Wu, Yubing Dai, Margaret Warner, Ivan Nalvarte, Per Antonson, Mukesh Varshney, Jan-Ake Gustafsson
Summary: The phenotype in the colon of aging LXR alpha beta (-/-) mice is attributed to the loss of ER beta expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Sukanya Ghosh, Manidipa Banerjee
Summary: By utilizing Flock House Virus-like particles as carriers and an easy chemical conjugation method, targeted delivery of hydrophobic drugs can be achieved effectively with dual functionality for improved bioavailability and reduced immune reactivity. The platform shows promising potential for biomedical applications.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Jing-Jing Shi, Hao-Fei Liu, Tian Hu, Xin Gao, Yong-Bin Zhang, Wei-Rong Li, Qi Wang, Shi-Jie Zhang, Dan Tang, Yun-Bo Chen
Summary: The study aimed to investigate the neuroprotective effects of Danggui-Shaoyao-San (DSS) on db/db mice with diabetes-induced cognitive dysfunction. Results showed that DSS treatment improved learning and memory abilities, relieved hippocampal neuronal damage, enhanced glucose tolerance and insulin resistance, reduced hyperlipemia, and increased antioxidant enzyme activities while decreasing inflammatory mediator levels. Additionally, DSS upregulated neurotrophic factors and decreased apoptosis in db/db mice.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Takahiro Masaki, Makoto Habara, Yuki Sato, Takahiro Goshima, Keisuke Maeda, Shunsuke Hanaki, Midori Shimada
Summary: The study reveals that calcineurin regulates the stability and activity of ER-alpha in breast cancer cells by modulating its phosphorylation at Ser 294 and Ser 118 positions. The expression of the calcineurin A-alpha gene (PPP3CA) is associated with poor prognosis in ER-alpha-positive breast cancer patients, suggesting calcineurin as a promising target for therapies in ER-alpha-positive breast cancer.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Psychology, Biological
Bryan V. Phillips-Farfan, Blanca Gomez Quintanar, Rebeca Reyes, Alonso Fernandez-Guasti
Summary: In this study, male rats treated with an aromatase inhibitor that preferred sexually experienced males showed higher expression of estrogen receptors alpha (ER α) and beta (ER β) in specific brain areas. This suggests that the unique brain characteristics of males with same-sex preference may be influenced by the expression of these hormone receptors, contributing to the biological underpinnings of sexual preference.
PHYSIOLOGY & BEHAVIOR
(2023)
Article
Endocrinology & Metabolism
Xin-Jun Wu, Marcus Jermaul Williams, Kimberly Ann Kew, Aubrey Converse, Peter Thomas, Yong Zhu
Summary: The study on a gper zebrafish mutant line suggests that Gper is involved in the control of ovarian follicle development through regulation of vitellogenesis and Egfr expression.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Yuri Park, Yeon Jean Cho, Nuri Sung, Mi Jin Park, Xiaoming Guan, William E. Gibbons, Bert W. O'Malley, Sang Jun Han
Summary: In this study, researchers identified a natural product called oleuropein that selectively inhibits ER beta activity without affecting ER alpha activity, thus suppressing the progression of endometriosis and improving fertility in patients.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Endocrinology & Metabolism
Sylvia C. Hewitt, San-pin Wu, Tianyuan Wang, Madhumita Ray, Marja Brolinson, Steven L. Young, Thomas E. Spencer, Alan DeCherney, Francesco J. DeMayo
Summary: This study investigates the molecular processes underlying estrogen responses and their effects on endometrial health. By analyzing the ESR1 cistromes and transcriptomes of endometrial biopsies and organoids, the study identifies candidate ESR1 target genes and reveals differences in the distribution and regulation of ESR1 binding sites between different stages of the menstrual cycle. The findings provide valuable resources for understanding how estrogen affects endometrial function and health.
Article
Endocrinology & Metabolism
Kelly L. Sams, Chinatsu Mukai, Brooke A. Marks, Chitvan Mittal, Elena Alina Demeter, Sophie Nelissen, Jennifer K. Grenier, Ann E. Tate, Faraz Ahmed, Scott A. Coonrod
Summary: By creating a Padi2/Padi4 double knockout (DKO) mouse model, this study found a series of abnormalities in DKO mice in terms of reproduction, such as longer time to sire litters, lighter weight, delayed puberty, lower testosterone levels, and smaller testes. This provides a new tool for investigating PAD function and the first in vivo evidence linking PAD proteins with hormone signaling.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Zachary C. Elmore, L. Patrick Havlik, Daniel K. Oh, Leif Anderson, George Daaboul, Garth W. Devlin, Heather A. Vincent, Aravind Asokan
Summary: The membrane-associated accessory protein (MAAP) is identified as a novel viral egress factor that promotes the secretion of AAV serotypes. MAAP contains a cationic amphipathic domain critical for AAV secretion. Restoring MAAP expression can rescue the secretion defect in various AAV serotypes with mutated start sites.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Dahae Lee, Young-Mi Kim, Young-Won Chin, Ki Sung Kang
Summary: This study demonstrated that schisandrol A has estrogen-like effects by inducing the expression of ER alpha through the PI3K/AKT and ERK signaling pathways, promoting the proliferation of MCF-7 cells. The effects of schisandrol A were more significant compared to E2 and could be mitigated by the ER antagonist ICI.
Article
Endocrinology & Metabolism
Clemence Delcour, Nahla Khawaja, Sergio Gonzalez-Duque, Sophie Lebon, Abir Talbi, Leila Drira, Chevenne Didier, Kamel Ajlouni, Nicolas de Roux
Summary: Estrogen receptor alpha dysfunction is a rare genetic disorder, with only a few cases reported to date. This study describes a new family with two sisters carrying the same rare ESR1 variant but showing different levels of endocrine and ovarian defects.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)