期刊
MOLECULAR SYSTEMS BIOLOGY
卷 8, 期 -, 页码 -出版社
WILEY
DOI: 10.1038/msb.2012.5
关键词
cell cycle; cell size; mass spectrometry; proteomics; T cells
资金
- Charles Wolfson Charitable Trust
- Leukaemia and Lymphoma Research (LLR)
- Medical Research Council (MRC)
- Elimination of Leukaemia Fund (ELF)
- Department of Trade and Industry (dti)
- Welch [F1515]
- Packard Foundations
- National Institutes of Health (NIH)
Regulating the transition of cells such as T lymphocytes from quiescence (G(0)) into an activated, proliferating state involves initiation of cellular programs resulting in entry into the cell cycle (proliferation), the growth cycle (blastogenesis, cell size) and effector (functional) activation. We show the first proteomic analysis of protein interaction networks activated during entry into the first cell cycle from G(0). We also provide proof of principle that blastogenesis and proliferation programs are separable in primary human Tcells. We employed a proteomic profiling method to identify large-scale changes in chromatin/nuclear matrix-bound and unbound proteins in human T lymphocytes during the transition from G(0) into the first cell cycle and mapped them to form functionally annotated, dynamic protein interaction networks. Inhibiting the induction of two proteins involved in two of the most significantly upregulated cellular processes, ribosome biogenesis (eIF6) and hnRNA splicing (SF3B2/SF3B4), showed, respectively, that human T cells can enter the cell cycle without growing in size, or increase in size without entering the cell cycle. Molecular Systems Biology 8: 573; published online 13 March 2012; doi:10.1038/msb.2012.5 Subject Categories: proteomics; cell cycle
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