Article
Reproductive Biology
Wen-Jie Yu, Cheng-Zhen Chen, Yan-Xia Peng, Ze Li, Yan Gao, Shuang Liang, Bao Yuan, Nam-Hyung Kim, Hao Jiang, Jia-Bao Zhang
Summary: SAL significantly improves porcine preimplantation embryo development by increasing blastocyst formation rate, proliferation ability, and mitochondrial activity while reducing ROS accumulation and apoptosis levels. This improvement is achieved through the reduction of ROS accumulation.
Article
Cell Biology
Zhaojun Geng, Yongxun Jin, Fushi Quan, Siyi Huang, Shuming Shi, Bing Hu, Zhichao Chi, Ilkeun Kong, Mingjun Zhang, Xianfeng Yu
Summary: This study found that MXC has significant effects on the development of early porcine embryos, leading to decreased cleavage rate, blastocyst rate, hatching rate, and total cell count. Moreover, MXC increases the production of reactive oxygen species, decreases mitochondrial membrane potential, and reduces mitochondrial copy number during early embryo development. Furthermore, MXC treatment leads to increased blastocyst apoptosis and DNA damage, decreased cell proliferation, and altered expression of pluripotency-related and apoptosis-related genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tiantong Qiu, Xin Chen, Peng Xiao, Liqiang Wang, Wenhua Li
Summary: In this study, the potential toxicity and underlying mechanisms of FLU on aquatic organisms were investigated using zebrafish embryos as a model organism. The results showed that FLU can cause microphthalmia in zebrafish embryos by inducing retinal cell apoptosis, indicating its developmental toxicity.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Environmental Sciences
Milton Talukder, Shao-Shuai Bi, Mei-Wei Lv, Jing Ge, Cong Zhang, Jin-Long Li
Summary: Cadmium exposure induces neuronal damage in the chicken brain, triggering the heat shock response (HSR) pathway and apoptotic pathway in response to the damage.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Cell Biology
Klaudia Mietkiewska, Pawel Kordowitzki, Chandra S. Pareek
Summary: Heat stress poses a significant threat to cattle reproduction, impacting hormonal balance, oocyte quality, mitochondrial function, DNA integrity, and maternal transcription. It can also lead to blastocyst development disorders. Cows exposed to heat stress in utero experience lower milk yield, immunological disorders, lower birth weight, and shortened lifespan associated with accelerated aging. Understanding the effects of heat stress at the cellular and molecular levels is crucial for protecting cow reproduction.
Article
Cell Biology
Dongjie Zhou, Ming-Hong Sun, Wen-Jie Jiang, Xiao-Han Li, Song-Hee Lee, Geun Heo, Jungseok Choi, Kwan-Suk Kim, Xiang-Shun Cui
Summary: In this study, the mRNA level of YME1L1 was knocked down in pig embryos and the expression patterns of YME1L1 and related proteins were examined. It was found that YME1L1 is localized in the punctate structures of mitochondria and highly expressed from the 4-cell stage. Knocking down YME1L1 led to decreased blastocyst rate and quality, mitochondrial fragmentation, excessive ROS production, lower mitochondrial membrane potential and ATP levels. Moreover, YME1L1 knockdown induced OPA1 cleavage and cytochrome c release. These findings demonstrate that YME1L1 is crucial for regulating mitochondrial fission, function, and apoptosis during porcine embryo preimplantation development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Veterinary Sciences
Giuseppe Caruso, Elena Maria Scalisi, Roberta Pecoraro, Vincenzo Cardaci, Anna Privitera, Emanuela Truglio, Fabiano Capparucci, Romana Jarosova, Antonio Salvaggio, Filippo Caraci, Maria Violetta Brundo
Summary: Oxidative stress is caused by an imbalance between pro-oxidants and antioxidants/antioxidant system. Excessive production of reactive oxygen species (ROS) and nitrogen species (RNS) has been linked to the development of diseases. Carnosine, a naturally occurring dipeptide, has shown the ability to scavenge ROS and inhibit protein oxidation. In this study, the effects of carnosine and titanium dioxide nanoparticles (TiO2-NPs) on zebrafish embryos/larvae were investigated, and it was found that carnosine could reduce TiO2-NPs-induced oxidative stress.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Chemistry, Medicinal
Andrew J. Ambrose, Jared Sivinski, Christopher J. Zerio, Xiaoyi Zhu, Jack Godek, Vlad K. Kumirov, Teresa Coma Brujas, Joan Torra Garcia, Anandhan Annadurai, Cody J. Schmidlin, Alyssa Werner, Taoda Shi, Reza Beheshti Zavareh, Luke Lairson, Donna D. Zhang, Eli Chapman
Summary: A recent study found that a fluorescence polarization assay can identify substrate-competitive Hsp70 inhibitors with isoform selectivity. In this study, the assay was used in a moderate-throughput screen to discover a druglike amino-acid-based inhibitor that specifically targets the endoplasmic reticular Hsp70, Grp78. The potency and selectivity of the inhibitor were further optimized through structure-activity relationship studies, but it showed little effect on cancer cell lines until it was combined with endoplasmic reticulum stress-inducing agents. The inhibitor demonstrated better potency in a spheroid tumor model compared to two-dimensional models. These findings represent the first reported isoform-selective small-molecule-competitive inhibitors of an Hsp70-substrate interaction.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Amit Haron, Mark Ruzal, Dmitry Shinder, Shelly Druyan
Summary: Moderate hypoxia plays a beneficial role in fetal development by triggering adaptive responses and activating genes crucial for cell and tissue adaptation to low oxygen conditions. Chicken embryos exhibit a dose-response effect to hypoxia, with varied levels impacting multiple mechanisms and enhancing developmental plasticity.
Article
Plant Sciences
Tung-Yi Lin, Wei-Jyun Hua, Hsin Yeh, Ai-Jung Tseng
Summary: The study demonstrates that FIPs LZ-8 and GMI induce changes in the proteomic profile of tumor lesions in LLC1 cell-bearing mouse, potentially regulating heat shock proteins (HSPs)-related cell viability and inhibiting cell migration and inducing apoptosis. Inhibition of HSPs may contribute to the anti-lung cancer activity of FIPs.
Article
Biochemistry & Molecular Biology
Tong Wu, Yanan Sheng, Yu Tian, Changyuan Wang
Summary: The study found that vitexin can alleviate heat stress and reduce cell apoptosis. Vitexin protects cells from heat stress-induced apoptosis by inhibiting intracellular reactive oxygen species levels and modulating the expression of heat shock proteins. This study provides a theoretical basis for the prevention and amelioration of heat stress using vitexin.
Article
Reproductive Biology
I. Yousefian, A. Zare-Shahneh, A. Goodarzi, H. Baghshahi, A. A. Fouladi-Nashta
Summary: Adding MitoTEMPO significantly increased the proportion of oocytes at the MII stage and embryos at the blastocyst stage while decreasing intracellular GSH content and ROS levels in oocytes. Furthermore, the expression level of the pro-apoptotic gene BAX was reduced in blastocysts from the M1 group compared to the control group.
Article
Multidisciplinary Sciences
Cheng-Han Lee, Tzu-Cheng Su, Ming-Sheng Lee, Chien-Sheng Hsu, Rei-Cheng Yang, Jun-Kai Kao
Summary: Hyperoxia is significantly involved in the development of lung injury, such as bronchopulmonary dysplasia (BPD), in premature infants or newborns. This study explored the potential of heat shock protein 70 (Hsp70) to prevent hyperoxia-induced lung injury in neonatal rats. The results showed that Hsp70 reduced early apoptosis of alveolar cells and decreased macrophage infiltration in the lung.
Article
Biochemistry & Molecular Biology
Shehu Sidi, Osvaldo Bogado Pascottini, Daniel Angel-Velez, Nima Azari-Dolatabad, Krishna Chaitanya Pavani, Gretania Residiwati, Tim Meese, Filip Van Nieuwerburgh, Elias Kambai Bawa, Ambrose Alikidon Voh, Joseph Olusegun Ayo, Ann Van Soom
Summary: Lycopene supplementation in atmospheric oxygen conditions during in vitro maturation of bovine oocytes has antioxidant effects, reducing oxidative stress and improving embryo development and quality. Transcriptomic analysis revealed differentially expressed genes associated with cellular function, metabolism, DNA repair, and anti-apoptosis, indicating the modulation of key pathways by lycopene.
Review
Medicine, Research & Experimental
Yeh Chin, Khanisyah E. Gumilar, Xing-Guo Li, Brahmana A. Tjokroprawiro, Chien-Hsing Lu, Jianrong Lu, Ming Zhou, Robert W. Sobol, Ming Tan
Summary: HSF1 is a master regulator of heat shock responsive signaling and also regulates a non-heat shock responsive transcriptional network. It plays important roles in cellular transformation and cancer development. Research on HSF1 has been active due to its critical functions in handling stressful cellular states. New functions and molecular mechanisms have been continuously discovered, providing new targets for cancer treatment strategies. This article reviews the essential roles and mechanisms of HSF1 in cancer cells, focusing on recently discovered functions and their underlying mechanisms, as well as advances in HSF1 inhibitors for cancer drug development.
Article
Agriculture, Dairy & Animal Science
Yao Xiao, Froylan Sosa, Lesley R. de Armas, Li Pan, Peter J. Hansen
Summary: Gene expression analysis in preimplantation embryos using a direct synthesis and specific-target pre-amplification method showed reliable results for sexing and other gene expression studies. Calibration curve analysis of PCR results validated 93.75% of genes tested, demonstrating the method's robustness. The study also showed that within-assay variation increased when cycle threshold values exceeded 18, indicating limitations in sensitivity at higher levels of gene expression.
JOURNAL OF DAIRY SCIENCE
(2021)
Editorial Material
Reproductive Biology
Peter J. Hansen, Marc-Antoine Driancourt
Article
Biochemistry & Molecular Biology
Eliab Estrada-Cortes, William Ortiz, Maria B. Rabaglino, Jeremy Block, Owen Rae, Elizabeth A. Jannaman, Yao Xiao, Peter J. Hansen
Summary: Provision of the methyl-donor choline to preimplantation embryos can alter their developmental program to increase gestation length, birth weight, and weaning weight, and cause postnatal changes in muscle DNA methylation associated with genes related to anabolic processes and cellular growth, highlighting the importance of embryo nutritional status for health and well-being after birth.
Article
Developmental Biology
Viju Vijayan Pillai, Tiffany G. Kei, Shailesh Gurung, Moubani Das, Luiz G. B. Siqueira, Soon Hon Cheong, Peter J. Hansen, Vimal Selvaraj
Summary: TGF beta 1 plays a crucial role in the differentiation of trophoblast cells, and inhibition of RhoA/Rock promotes self-renewal and proliferation.
Article
Multidisciplinary Sciences
Thiago F. Amaral, Joao Gabriel Viana de Grazia, Luany Alves Galvao Martinhao, Felipe De Col, Luiz Gustavo B. Siqueira, Joao Henrique M. Viana, Peter J. Hansen
Summary: The study aimed to optimize in vitro embryo production in cattle. The results showed that the proportion of embryos becoming blastocysts in culture was influenced by the type of serum in the medium and the addition of specific embryokines. The sire also had a significant effect on embryonic development and pregnancy rate.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
L. M. Jensen, E. A. Jannaman, J. E. Pryce, A. De Vries, P. J. Hansen
Summary: Heat stress has negative consequences for milk production and reproduction of dairy cattle, and there is genetic variation among cows in their ability to resist these effects. This study evaluated the effectiveness of the Australian breeding value for heat tolerance (ABVHT) in predicting cow differences in the effects of heat stress on body temperature regulation, milk production, and reproductive function.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Erly Luisana Carrascal-Triana, Adriana Moreira Zolini, Antonio Ruiz de King, Jurandy Mauro Penitente-Filho, Peter J. Hansen, Ciro Alexandre Alves Torres, Jeremy Block
Summary: This study investigated the effects of supplementation with ascorbate, dithiothreitol (DTT) or caspase-3 inhibitor (z-DEVD-fmk) on the post-thaw survival of in vitro-produced bovine embryos. The results showed that the addition of 0.1 mM ascorbate to the freezing medium improved re-expansion rates and hatching rate of the embryos, while reducing intracellular ROS levels and DNA fragmentation.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Cell Biology
Froylan Sosa, Peter J. Hansen
Summary: The study found that CSF2 can protect the developmental competence of bovine embryos under heat shock, especially at the zygote and two-cell stages.
Article
Biochemistry & Molecular Biology
Maria Belen Rabaglino, Dessie Salilew-Wondim, Adriana Zolini, Dawit Tesfaye, Michael Hoelker, Pat Lonergan, Peter J. J. Hansen
Summary: This study developed a gene signature model to predict embryonic competence for survival by integrating transcriptomic data from blastocysts and elongating conceptuses. The potential biomarkers were validated with independent embryonic data sets using machine-learning algorithms. The results showed that the predictions of embryonic development fate based on these biomarkers were highly accurate.
Article
Agriculture, Dairy & Animal Science
Camila J. Cuellar, Muhammad Saleem, L. M. Jensen, P. J. Hansen
Summary: This study investigates the relationship between the ability of cattle to regulate body temperature during heat stress and their milk production capacity, as well as the impact of genetic groups on the seasonal depression in milk yield. The findings suggest that Brown Swiss and crossbred cows have better body temperature regulation during heat stress compared to Holsteins, but they are not more resistant to heat stress in terms of milk yield. Therefore, genetic differences in thermotolerance may exist independently of regulation of body temperature.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Reproductive Biology
Froylan Sosa, Kyungjun Uh, Jessica N. Drum, Katy S. Stoecklein, Kimberly M. Davenport, M. Sofia Ortega, Kiho Lee, Peter J. Hansen
Summary: This study investigates the role of CSF2RA in the development of bovine embryos. The results show that inactivation of CSF2RA leads to lower development rates of embryos to the blastocyst stage and changes in gene expression. The CSF2 signaling pathway enhances the likelihood of blastocyst formation and affects functions such as cell signaling and glycosylation.
REPRODUCTION AND FERTILITY
(2023)
Article
Cell Biology
Quinn A. Hoorn, Maria Belen Rabaglino, Tatiane S. Maia, Masroor Sagheer, Dailin Fuego, Zongliang Jiang, Peter J. Hansen
Summary: The study characterizes the expression of genes encoding cell signaling ligands in bovine endosalpinx and endometrium and analyzes spatial changes in gene expression. It finds that these cell signaling ligands may influence early embryonic development and highlights the distinct expression profiles of the oviduct and endometrium.
PHYSIOLOGICAL GENOMICS
(2023)
Article
Developmental Biology
Daniel L. Stanton, Alexander Graf, Tatiane S. Maia, Helmut Blum, Zongliang Jiang, Peter J. Hansen
Summary: It is not known when a functional circadian clock is established in the developing embryo. Lack of expression of key genes involved in the clock mechanism is indicative that a functional circadian clock mechanism is absent in the mammalian preimplantation embryo through the blastocyst stage of development.
Article
Agriculture, Dairy & Animal Science
Quinn A. Hoorn, Gabriel A. Zayas, Eduardo E. Rodriguez, Laura M. Jensen, Raluca G. Mateescu, Peter J. Hansen
Summary: This study identified QTLs and candidate SNPs associated with pregnancy outcomes in beef heifers, including a large QTL associated with a group of protocadherin genes. Confirmation of these associations with larger populations could lead to the development of genomic predictions of reproductive function in beef cattle. Moreover, additional research is warranted to study the function of candidate genes associated with QTLs.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Peter J. Hansen
Summary: Graduate education is crucial for academic scientists as it carries the responsibility of guiding students towards their career and life goals. Being a good mentor involves developing an education framework and adapting it to each student's needs based on experience, advice, and individual personality. Most importantly, mentors should be invested in the success and well-being of their students.
JOURNAL OF ANIMAL SCIENCE
(2023)