Article
Neurosciences
Catherine S. Thomas, Aida Mohammadkhani, Madiha Rana, Min Qiao, Corey Baimel, Stephanie L. Borgland
Summary: Reward and reinforcement processes are critical for survival and propagation of genes, with mesolimbic dopamine playing a central role. Optogenetic stimulation of orexin/dynorphin inputs in the VTA potentiates dopamine release in the NAc core, resulting in real time and conditioned place preference as well as increased food cue-directed orientation. While dynorphin contributes to aversive conditioning, the rewarding effects of LH orexin/dynorphin stimulation in the VTA are primarily driven by orexin rather than dynorphin.
NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Optics
Hiroki Yasunaga, Hibiki Takeuchi, Koyo Mizuguchi, Atsushi Nishikawa, Alexander Loesing, Mikiko Ishikawa, Chikako Kamiyoshihara, Susumu Setogawa, Noriaki Ohkawa, Hiroto Sekiguchi
Summary: This study investigates the temperature rise characteristics in brain tissue using a MicroLED probe. The findings show that the temperature rise strongly depends on the surrounding environment and the area of contact. Additionally, a new method for in situ temperature monitoring using the electrical characteristics of the MicroLED is proposed. Furthermore, the study demonstrates the effective control of optogenetic neural activity through optical stimulation by MicroLEDs.
Article
Biochemistry & Molecular Biology
Reza Azadi, Simon Bohn, Emily Lopez, Rosa Lafer-Sousa, Karen Wang, Mark A. G. Eldridge, Arash Afraz
Summary: Artificial activation of neurons in early visual areas induces perception of simple visual flashes. Stimulation in high-level visual cortices is expected to induce perception of complex features. However, studies in human patients have shown that stimulation rarely induces detectable visual events, and stimulation of specific cortices only leads to hallucinations when subjects are looking at relevant objects. Anecdotal observations suggest that stimulation of high-level visual cortex can evoke perception of complex visual features, but these effects depend on the availability and content of visual input.
Article
Neurosciences
Wei-wei Deng, Guang-yan Wu, Ling-xia Min, Zhou Feng, Hui Chen, Ming-liang Tan, Jian-feng Sui, Hong-liang Liu, Jing-ming Hou
Summary: Activation of glutamate neurons in the primary motor cortex can significantly improve motor function scores in rats, shorten the incubation period of motor evoked potentials, and increase motor potentials' amplitude. Accurate activation of the primary motor cortex can effectively promote tissue recovery and neurofilament growth, while also increasing the content of some growth-related proteins at the injured site. These findings suggest that selective activation of glutamate neurons in the primary motor cortex may play a significant role in promoting functional recovery after SCI and understanding the neural cell mechanism involved in recovery induced by motor cortex stimulation.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Cell Biology
Brendan Ryu, Shivathmihai Nagappan, Fernando Santos-Valencia, Psyche Lee, Erica Rodriguez, Meredith Lackie, Jun Takatoh, Kevin M. Franks
Summary: Using optogenetics, researchers found that brief activation of targeted populations of mouse PCx principal neurons can trigger massive, generalized seizures. Optokindling disrupts the balance of recurrent excitation and feedback inhibition mainly by causing a significant decrease in feedback inhibition.
Article
Neurosciences
Joungha Won, Yuriy Pankratov, Minwoo Wendy Jang, Sunpil Kim, Yeon Ha Ju, Sangkyu Lee, Seung Eun Lee, Arie Kim, Soowon Park, C. Justin Lee, Won Do Heo
Summary: Spatiotemporal control of brain activity can be achieved using Opto-vTrap, which is a light-inducible and reversible system that temporarily inhibits the release of neurotransmitters. Opto-vTrap significantly reduces synaptic and gliotransmission, leading to inhibition of hippocampus-dependent memory retrieval.
Article
Clinical Neurology
Katerina Hristova, Cristina Martinez-Gonzalez, Thomas C. Watson, Neela K. Codadu, Kevan Hashemi, Peter C. Kind, Matthew F. Nolan, Alfredo Gonzalez-Sulser
Summary: This study evaluated a strategy of using optogenetic activation of medial septal GABAergic neurons to control seizures in temporal lobe epilepsy. The results showed that this method can reduce the duration of spontaneous seizures, while the number of medial septal GABAergic neurons and synaptic puncta in the hippocampus remained unchanged in epileptic conditions.
Article
Multidisciplinary Sciences
Xiao Yu, Guangchao Zhao, Dan Wang, Sa Wang, Rui Li, Ao Li, Huan Wang, Mathieu Nollet, You Young Chun, Tianyuan Zhao, Raquel Yustos, Huiming Li, Jianshuai Zhao, Jiannan Li, Min Cai, Alexei L. Vyssotski, Yulong Li, Hailong Dong, Nicholas P. Franks, William Wisden
Summary: Social defeat stress induces sleep in mice, and a subset of VTA(Vgat-Sst) cells plays a crucial role in promoting and maintaining sleep through a specific neural circuit, while also inhibiting CRF release after stress.
Article
Physiology
Gabriella L. Robilotto, Olivia J. Yang, Firoj Alom, Richard D. Johnson, Aaron D. Mickle
Summary: Urothelial cells play an important role in both barrier function and sensory function in the bladder. A mouse model using optogenetics was developed to directly stimulate urothelial cells and study their communication with sensory neurons.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Youjin Lee, Daeho Ryu, Saeyeong Jeon, Yena Lee, Yoon Kyung Cho, Chang-Hyeon Ji, Yong-Kweon Kim, Sang Beom Jun
Summary: Optogenetics is an essential tool for investigating neural signaling due to its unique capability of selective neural modulation or monitoring. The ability to genetically modify neuronal cells to express opsin proteins enables optical stimulation or inhibition of specific neurons. Recent advancements include combining optical waveguides for light delivery with electrophysiological recording to monitor neural responses to optogenetic stimuli simultaneously.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Clinical Neurology
Lindsea C. Booth, Song T. Yao, Alla Korsak, David G. S. Farmer, Sally G. Hood, Daniel McCormick, Quinn Boesley, Angela A. Connelly, Stuart J. McDougall, Willian S. Korim, Sarah-Jane Guild, Svetlana Mastitskaya, Phuong Le, Anja G. Teschemacher, Sergey Kasparov, Gareth L. Ackland, Simon C. Malpas, Robin M. McAllen, Andrew M. Allen, Clive N. May, Alexander V. Gourine
Summary: This study successfully applied optogenetics for selective stimulation of vagal efferent activity in a large animal model (sheep). Viral transduction of a subset of vagal motoneurons resulted in strong axonal membrane expression of the excitatory light-sensitive ion channel ChIEF in efferent projections innervating thoracic organs and reaching beyond the diaphragm.
Review
Biotechnology & Applied Microbiology
Sant Kumar, Mustafa Khammash
Summary: Harnessing the potential of optogenetics in biology requires methodologies from different disciplines and suitable light stimulation platforms. This review explores the evolution of light-induction hardware-software platforms, discusses their advantages and disadvantages, and examines design approaches in optogenetic experiments. The development of automated measurement and stimulation schemes on these platforms enables computer-controlled living systems.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mochi Liu, Sandeep Kumar, Anuj K. Sharma, Andrew M. Leifer
Summary: We introduce a high-throughput optogenetic illumination system that allows simultaneous closed-loop light delivery to specific targets in moving Caenorhabditis elegans populations. The system addresses technical challenges of targeted illumination, automatic stimuli delivery based on behavior, and high throughput targeting of multiple animals at once. The system was used to investigate the behavioral response of the animals to mechanosensory stimuli, measuring responses to over 43,418 stimulus events. The results provide insights into the animals' probability of forward sprinting and reversing in response to different stimulation intensities and confirm previous findings regarding the gating effect of turning on mechanosensory evoked reversals.
Article
Cell Biology
Aiste Baleisyte, Ralf Schneggenburger, Olexiy Kochubey
Summary: The medial amygdala (MeA) plays a crucial role in social behaviors by receiving pheromone information. A study reveals that the behavioral outcome differs when activating GABA neurons in the postero-dorsal MeA (MeApd) using different channelrhodopsin variants. The mechanism behind this paradoxical difference is not yet understood.
Article
Engineering, Biomedical
Yohei Kawana, Junta Imai, Yosuke M. Morizawa, Yoko Ikoma, Masato Kohata, Hiroshi Komamura, Toshihiro Sato, Tomohito Izumi, Junpei Yamamoto, Akira Endo, Hiroto Sugawara, Haremaru Kubo, Shinichiro Hosaka, Yuichiro Munakata, Yoichiro Asai, Shinjiro Kodama, Kei Takahashi, Keizo Kaneko, Shojiro Sawada, Tetsuya Yamada, Akira Ito, Kuniyasu Niizuma, Teiji Tominaga, Akihiro Yamanaka, Ko Matsui, Hideki Katagiri
Summary: The study presents two optogenetic methods for stimulating the vagal nerve, leading to enhanced glucose-stimulated insulin secretion and beta cell proliferation in mice expressing choline acetyltransferase-channelrhodopsin 2.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Neurosciences
Scellig S. D. Stone, Catia M. Teixeira, Kirill Zaslavsky, Anne L. Wheeler, Alonso Martinez-Canabal, Afra H. Wang, Masanori Sakaguchi, Andres M. Lozano, Paul W. Frankland
Article
Neurosciences
Maria Llorens-Martin, Catia M. Teixeira, Almudena Fuster-Matanzo, Jeronimo Jurado-Arjona, Victor Borrell, Eduardo Soriano, Jesus Avila, Felix Hernandez
JOURNAL OF ALZHEIMERS DISEASE
(2012)
Article
Neurosciences
Catia M. Teixeira, Michelle M. Kron, Nuria Masachs, Helen Zhang, Diane C. Lagace, Albert Martinez, Isabel Reillo, Xin Duan, Carles Bosch, Lluis Pujadas, Lucas Brunso, Hongjun Song, Amelia J. Eisch, Victor Borrell, Brian W. Howell, Jack M. Parent, Eduardo Soriano
JOURNAL OF NEUROSCIENCE
(2012)
Article
Neurosciences
Tahilia J. Rebello, Qinghui Yu, Nathalie M. Goodfellow, Martha K. Caffrey Cagliostro, Anne Teissier, Emanuela Morelli, Elena Y. Demireva, Alexei Chemiakine, Gorazd B. Rosoklija, Andrew J. Dwork, Evelyn K. Lambe, Jay A. Gingrich, Mark S. Ansorge
JOURNAL OF NEUROSCIENCE
(2014)
Editorial Material
Biochemistry & Molecular Biology
M. Llorens-Martin, A. Fuster-Matanzo, C. M. Teixeira, J. Jurado-Arjona, F. Ulloa, J. deFelipe, A. Rabano, F. Hernandez, E. Soriano, J. Avila
MOLECULAR PSYCHIATRY
(2013)
Article
Biochemistry & Molecular Biology
M. Llorens-Martin, A. Fuster-Matanzo, C. M. Teixeira, J. Jurado-Arjona, F. Ulloa, J. deFelipe, A. Rabano, F. Hernandez, E. Soriano, J. Avila
MOLECULAR PSYCHIATRY
(2013)
Article
Biochemistry & Molecular Biology
Q. Yu, C. M. Teixeira, D. Mahadevia, Y. Huang, D. Balsam, J. J. Mann, J. A. Gingrich, M. S. Ansorge
MOLECULAR PSYCHIATRY
(2014)
Editorial Material
Clinical Neurology
Jean-Marie Girard, Scellig S. D. Stone, Hannes Lohi, Christophe Blaszykowski, Catia Teixeira, Julie Turnbull, Afra Wang, Arman Draginov, Peixiang Wang, Xiao Chu Zhao, Cameron A. Ackerley, Paul W. Frankland, Berge A. Minassian
Article
Neurosciences
Catia M. Teixeira, Nuria Masachs, Ashraf Muhaisen, Carles Bosch, Javier Perez-Martinez, Brian Howell, Eduardo Soriano
NEUROPSYCHOPHARMACOLOGY
(2014)
Review
Neurosciences
Deepika Suri, Catia M. Teixeira, Martha K. Caffrey Cagliostro, Darshini Mahadevia, Mark S. Ansorge
NEUROPSYCHOPHARMACOLOGY
(2015)
Article
Multidisciplinary Sciences
Lluis Pujadas, Daniela Rossi, Rosa Andres, Catia M. Teixeira, Bernat Serra-Vidal, Antoni Parcerisas, Rafael Maldonado, Ernest Giralt, Natalia Carulla, Eduardo Soriano
NATURE COMMUNICATIONS
(2014)
Article
Biochemical Research Methods
Anne L. Wheeler, Catia M. Teixeira, Afra H. Wang, Xuejian Xiong, Natasa Kovacevic, Jason P. Lerch, Anthony R. McIntosh, John Parkinson, Paul W. Frankland
PLOS COMPUTATIONAL BIOLOGY
(2013)
Review
Behavioral Sciences
Emmanuelle Courtiol, Edenia C. Menezes, Catia M. Teixeira
Summary: Serotonin plays a critical role in development and behavior, with its involvement in reward being a topic of debate. Classical studies using electrical stimulation and pharmacological approaches have produced contradictory results on the role of serotonin in reward. More recent optogenetic studies suggest that activation of the serotonergic cells in the Raphe to VTA pathway can mediate reinforcement learning through the excitation of dopaminergic neurons. Understanding the interaction between the serotonergic and dopaminergic systems is crucial for insights into neuropsychiatric disorders.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2021)
Article
Anatomy & Morphology
Carles Bosch, Albert Martinez, Nuria Masachs, Catia M. Teixeira, Isabel Fernaud, Fausto Ulloa, Esther Perez-Martinez, Carlos Lois, Joan X. Comella, Javier DeFelipe, Angel Merchan-Perez, Eduardo Soriano
FRONTIERS IN NEUROANATOMY
(2015)
