期刊
MOLECULAR PSYCHIATRY
卷 17, 期 10, 页码 1017-1025出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/mp.2011.160
关键词
3D optical angiography; 3D optical Doppler tomography; cocaine toxicity; microischemia
资金
- National Institutes of Health [K25-DA021200, 2R01-DK059265, 1RC1DA028534, 1R21-DA032228]
- NIAAA Intramural Research Program
Cocaine-induced stroke is among the most serious medical complications associated with its abuse. However, the extent to which acute cocaine may induce silent microischemia predisposing the cerebral tissue to neurotoxicity has not been investigated; in part, because of limitations of current neuroimaging tools, that is, lack of high spatiotemporal resolution and sensitivity to simultaneously measure cerebral blood flow (CBF) in vessels of different calibers (including capillaries) quantitatively and over a large field of view. Here we combine ultrahigh-resolution optical coherence tomography to enable tracker-free three-dimensional (3D) microvascular angiography and a new phase-intensity-mapping algorithm to enhance the sensitivity of 3D optical Doppler tomography for simultaneous capillary CBF quantization. We apply the technique to study the responses of cerebral microvascular networks to single and repeated cocaine administration in the mouse somatosensory cortex. We show that within 2-3 min after cocaine administration CBF markedly decreased (for example, similar to 70%), but the magnitude and recovery differed for the various types of vessels; arterioles had the fastest recovery (similar to 5 min), capillaries varied drastically (from 4-20 min) and venules showed relatively slower recovery (similar to 12 min). More importantly, we showed that cocaine interrupted CBF in some arteriolar branches for over 45 min and this effect was exacerbated with repeated cocaine administration. These results provide evidence that cocaine doses within the range administered by drug abusers induces cerebral microischemia and that these effects are exacerbated with repeated use. Thus, cocaine-induced microischemia is likely to be a contributor to its neurotoxic effects. Molecular Psychiatry (2012) 17, 1017-1025; doi:10.1038/mp.2011.160; published online 29 November 2011
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