Article
Plant Sciences
Prashantee Singh, Raj Kumar Verma, Subhadeep Chatterjee
Summary: The Xanthomonas group of phytopathogens communicate using a fatty acid-like signal molecule known as diffusible signal factor (DSF). In the rice pathogen, Xanthomonas oryzae pv. oryzae, DSF regulates virulence-associated functions including the secretion of cell wall hydrolysing type II effectors. Mutant analysis showed that DSF is required for membrane integrity in regulating the secretion of type II effectors and virulence. Increasing osmolarity could rescue the hypersecretion phenotype of the mutant.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Microbiology
Chao Yu, Doan-Phuong Nguyen, Fenghuan Yang, Jia Shi, Yiming Wei, Fang Tian, Xiuxiang Zhao, Huamin Chen
Summary: This study reveals the overlapping and unique regulatory roles of RpoN1 and RpoN2 in Xanthomonas oryzae pv. oryzae, shedding light on their mechanisms in bacterial motility, virulence, and growth.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xing Dai, Yankai Wang, Kaili Yu, Yonghui Zhao, Langyu Xiong, Ruozhong Wang, Shengben Li
Summary: The overexpression of OsNPR1 enhances rice resistance to Xanthomonas oryzae pv. oryzae (X-oo) by bidirectionally regulating gene expression in rice and X-oo. In X-oo-infected OsNPR1-OE plants, rice genes involved in cell wall biosynthesis, SA signaling pathways, and PR genes are upregulated, while X-oo genes involved in energy metabolism, oxidative phosphorylation, biosynthesis, and transportation are repressed. The downregulation of virulence genes, including components of type III and other secretion systems, is observed in X-oo due to OsNPR1 overexpression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Yichao Yan, Yanyan Wang, Xiaofei Yang, Yuan Fang, Guanyun Cheng, Lifang Zou, Gongyou Chen
Summary: The Min system in Xoo, a negative regulatory system for bacterial cell division, was found to be involved in the negative regulation of pathogenicity and other traits in Xoo. Mutations in the Min system resulted in enhanced expression of hrp genes, elongated cell lengths, impaired virulence, and enhanced biofilm formation. This is the first report indicating the involvement of the Min system in the regulation of T3SS expression in Xoo, providing new insights into Xoo's virulence mechanisms.
Article
Microbiology
Weiwei Yan, Yiming Wei, Susu Fan, Chao Yu, Fang Tian, Qi Wang, Fenghuan Yang, Huamin Chen
Summary: The study characterized the GGDEF domain protein GdpX6 in the rice blight pathogen Xoo, demonstrating its DGC activity and its role in controlling virulence, swimming and sliding motility, and biofilm formation.
Article
Virology
Muchen Zhang, Jiahui Qian, Xinyan Xu, Temoor Ahmed, Yong Yang, Chenqi Yan, Mohsen Mohamed Elsharkawy, Mohamed M. Hassan, Jamal A. Alorabi, Jianping Chen, Bin Li
Summary: This study reveals the significance of glycosyltransferase in interactions among pathogenic bacteria, phages, and plant hosts, shedding light on the balanced coevolution from an environmental perspective.
Article
Plant Sciences
Hyeran Moon, A-Ram Jeong, Oh-Kyu Kwon, Chang-Jin Park
Summary: Orphan genes are commonly found in genomes, but their functions are largely unknown. In this study, a defense-related orphan gene Xio1 was identified and found to play an important role in rice's defense mechanism, providing a new resource for crop breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Pei-hong WANG, Sai WANG, Wen-han NIE, Yan WU, Iftikhar Ahmad, Ayizekeranmu Yiming, Jin HUANG, Gong-you CHEN, Bo ZHU
Summary: Horizontal gene transfer (HGT) plays an important role in the evolution of bacteria. This study reveals the regulatory mechanism of xoc_2868, a horizontally acquired gene in Xanthomonas oryzae pv. oryzicola strain BLS256. The results show that xoc_2868 regulates the expression of genes involved in extracellular polysaccharide production and xanthan biosynthesis, as well as cytochrome bd oxidase. Knockout mutants of xoc_2868 and cydAB showed reduced bacterial virulence and increased sensitivity to H2O2. These findings provide new insights into the regulatory pathways and evolution of bacteria.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Biochemistry & Molecular Biology
Seungmin Son, Jong Hee Im, Giha Song, Suhyeon Nam, Sang Ryeol Park
Summary: The phytohormone abscisic acid (ABA) regulates the immune response of rice to Xanthomonas oryzae pv. oryzae (Xoo). This study identified OsWRKY114 as a negative regulator of ABA, reducing the susceptibility of rice to Xoo infection and affecting the expression of ABA-associated genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Hongxia Lu, Zhongjie Shen, Yujun Xu, Linjing Wu, Deyu Hu, Runjiang Song, Baoan Song
Summary: This study found that ethylicin (ET) inhibited the proliferation of Xanthomonas oryzae pv. oryzae (Xoo) by increasing the content of defense enzymes and chlorophyll in rice. It was also discovered that ET activated the abscisic acid (ABA) signal pathway and made the protein kinase OsCPK24 more active, enhancing the resistance of rice to Xoo.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Yaze Kong, Gang Wang, Xian Chen, Linying Li, Xueying Zhang, Sangtian Chen, Yuqing He, Gaojie Hong
Summary: Phosphate and MYC2-mediated JA pathway play crucial roles in plant growth and development. In rice, Pi starvation activates OsMYC2 signaling to enhance resistance to Xoo infection, with the direct regulation of OsPHR2 on OsMYC2 promoter confirmed. These findings highlight a molecular link between OsMYC2-mediated JA pathway and phosphate starvation signaling in monocot plants.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Microbiology
Yu-Qiang Zhang, Xiao-Yan Song, Fengquan Liu
Summary: This study identified a novel ferric uptake regulator (Fur) protein, XanFur, in Xanthomonas oryzae pv. oryzae (Xoo), which plays important roles in the pathogenicity and oxidative stress resistance of Xoo. Furthermore, the study revealed that the global transcriptional regulator Clp positively regulates the expression of xanfur. These findings contribute to a better understanding of the interaction mechanisms between rice and Xoo, and provide reference for the development of effective bactericides targeting the Fur protein to control bacterial leaf blight in rice.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Mengju Liu, Ye Tian, Haitham E. M. Zaki, Temoor Ahmed, Rong Yao, Chengqi Yan, Sebastian Leptihn, Belinda Loh, Muhammad Shafiq Shahid, Fang Wang, Jianping Chen, Bin Li
Summary: This study aimed to investigate the co-evolution of bacterial pathogens with their host plants as well as with their bacterial viruses. Through experiments, several genes associated with both phage sensitivity and host pathogenicity were identified, and three genes were found to play a key role in bacterial virulence and phage resistance.
Article
Biology
Van Schepler-Luu, Coline Sciallano, Melissa Stiebner, Chonghui Ji, Gabriel Boulard, Amadou Diallo, Florence Auguy, Si Nian Char, Yugander Arra, Kyrylo Schenstnyi, Marcel Buchholzer, Eliza P. I. Loo, Atugonza L. Bilaro, David Lihepanyama, Mohammed Mkuya, Rosemary Murori, Ricardo Oliva, Sebastien Cunnac, Bing Yang, Boris Szurek, Wolf B. Frommer
Summary: Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), poses a threat to global food security and the livelihood of small-scale rice producers. Despite robust global rice trade, Xoo strains from different regions show complete continental segregation. Unprecedented BB outbreaks were discovered in Tanzania, caused by Xoo strains carrying Asian-type TAL effectors and iTAL effectors, unlike endemic African Xoo strains. In order to protect African rice production from this emerging threat, a hybrid CRISPR-Cas9/Cpf1 system was developed to edit TALe-binding elements in SWEET promoters. The edited lines displayed broad-spectrum resistance against Asian and African Xoo strains.
Article
Microbiology
Huihui Wang, Fan Chen, Guiyu Tang, Wenli Ke, Shasha Wang, Dehong Zheng, Lifang Ruan
Summary: In this study, we identified a transcriptional regulator called Gar (PXO_RS11965) that is involved in the regulation of bacterial growth and virulence in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight in rice. Knockout of gar increased Xoo's virulence to rice, and RNA-sequencing and GUS assays showed that Gar positively regulates the expression of a σ54 factor called rpoN2. Further experiments confirmed that overexpression of rpoN2 restored the phenotypic changes caused by gar deletion. Our research revealed that Gar influences bacterial growth and virulence by positively regulating the expression of rpoN2.
CURRENT MICROBIOLOGY
(2023)
Article
Plant Sciences
Sheo Shankar Pandey, Pradeep Kumar Patnana, Rikky Rai, Subhadeep Chatterjee
MOLECULAR PLANT PATHOLOGY
(2017)
Article
Plant Sciences
Raj Kumar Verma, Biswajit Samal, Subhadeep Chatterjee
MOLECULAR PLANT PATHOLOGY
(2018)
Article
Environmental Sciences
Sheo Shankar Pandey, Pradeep Kumar Patnana, Yasobanta Padhi, Subhadeep Chatterjee
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2018)
Article
Genetics & Heredity
Biswajit Samal, Subhadeep Chatterjee
Article
Mycology
Pooja Sethiya, Maruti Nandan Rai, Rikky Rai, Chirag Parsania, Kaeling Tan, Koon Ho Wong
Article
Plant Sciences
Biswajit Samal, Subhadeep Chatterjee
Summary: The study revealed the important role of quorum sensing in phytopathogens, facilitating Xanthomonas campestris colonization in cabbage leaves and triggering chlorosis, promoting systemic infection. The quorum sensing response in the Xanthomonas group maximizes population fitness, triggering host chlorophagy at specific stages and establishing systemic infection.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Rikky Rai, Julius Pasion, Tanvi Majumdar, Cory E. Green, Sarah R. Hind
Summary: The study used genomics and genetics approaches to elucidate the pathogenesis mechanisms of Xanthomonas cucurbitae, revealing a smaller genome size with fewer virulence-related genes compared to other Xanthomonas species. RNA-seq analysis showed altered transcriptional responses of X. cucurbitae under plant-mimicking conditions, with upregulation of virulence genes and downregulation of cellular homeostasis genes. Additionally, both type II enzymes and type III effectors were found to be necessary for X. cucurbitae infection in pumpkin hosts through gene deletion methods.
Article
Microbiology
Akanksha Kakkar, Raj Kumar Verma, Biswajit Samal, Subhadeep Chatterjee
Summary: The study found that four Xoo proteins play important roles in maintaining c-di-GMP abundance and regulating virulence-associated functions, particularly the GGDEF domain of Xoo2563 affects the virulence-associated functions of Xoo. Contrasting experiments showed that deletion of the GGDEF domain resulted in altered iron metabolism, highlighting the complex role of c-di-GMP signaling in coordinating virulence-associated functions in Xoo.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Plant Sciences
Sheo Shankar Pandey, Subhadeep Chatterjee
Summary: This review discusses the role of cell-to-cell signaling and iron in the regulation of virulence-associated functions in Xanthomonas plant pathogens. Xanthomonas strains exhibit atypical patterns of regulation, which contribute to their adaptation to different lifestyles.
Article
Plant Sciences
Prashantee Singh, Raj Kumar Verma, Subhadeep Chatterjee
Summary: The Xanthomonas group of phytopathogens communicate using a fatty acid-like signal molecule known as diffusible signal factor (DSF). In the rice pathogen, Xanthomonas oryzae pv. oryzae, DSF regulates virulence-associated functions including the secretion of cell wall hydrolysing type II effectors. Mutant analysis showed that DSF is required for membrane integrity in regulating the secretion of type II effectors and virulence. Increasing osmolarity could rescue the hypersecretion phenotype of the mutant.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Immunology
Maruti Nandan Rai, Chirag Parsania, Rikky Rai
Summary: This study investigated the interaction between Candida albicans and host macrophages using a human macrophage model. The results showed that C. albicans responds differently to human macrophages compared to murine macrophages, and induces a proinflammatory response in macrophages by upregulating genes associated with cell surface-mediated signaling.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Maruti Nandan Rai, Rikky Rai, Pooja Sethiya, Chirag Parsania
Summary: Candida glabrata, an opportunistic fungal pathogen, has a diverse transcriptional response to different environmental stresses, with a common adaptive transcriptional response observed. Transcriptional regulatory association networks reveal potential transcription factors involved in the regulation of adaptive response genes.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Microbiology
Yasobanta Padhi, Subhadeep Chatterjee
Summary: In this study, a novel DedA protein XdfA was identified in Xanthomonas campestris, which is involved in membrane homeostasis, magnesium transport, and virulence. Understanding the role of XdfA in bacterial physiology and its connection to plant protection will provide effective antimicrobial strategies for horticulturally and agriculturally important crop plants.
Article
Biochemistry & Molecular Biology
Sreegowrinadh Javvadi, Sheo Shankar Pandey, Amita Mishra, Binod Bihari Pradhan, Subhadeep Chatterjee
Article
Biology
Sheo Shankar Pandey, Prashantee Singh, Biswajit Samal, Raj Kumar Verma, Subhadeep Chatterjee