Article
Biotechnology & Applied Microbiology
Tomohiko Matsuzawa, Masahiro Watanabe, Yusuke Nakamichi, Akihiko Kameyama, Naoshi Kojima, Katsuro Yaoi
Summary: In this study, an extracellular alpha-xylosidase, AxyB, was identified and characterized in Aspergillus oryzae, belonging to glycoside hydrolase family 31 and involved in releasing D-xylose from xyloglucan oligosaccharides. The research shows that AxyB plays a key role in the degradation of xyloglucans by releasing the xylopyranosyl side chain from these oligosaccharides.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chengyu Liu, Ningning Shen, Qian Zhang, Minghui Qin, Tingyan Cao, Shuai Zhu, Dingzhong Tang, Libo Han
Summary: This study identified a transcription factor called MoBZIP3 that plays a crucial role in the pathogenesis of the devastating fungus Magnaporthe oryzae. MoBZIP3 regulates the formation of specialized infection structure appressorium by controlling the utilization of glycogen and lipid droplets for glycerol generation. It also regulates the expression of genes related to glycerol synthesis critical for appressorium turgor pressure generation. Additionally, MoBZIP3 is involved in the regulation of multiple signaling pathways including the turgor sensor gene and actin assembly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Na Ning, Xin Xie, Haiyue Yu, Jie Mei, Qianqian Li, Shimin Zuo, Hanxiang Wu, Wende Liu, Zhiqiang Li
Summary: In this study, we identified MoPtep1 as an important pathogenic effector in rice blast. It localized in plant peroxisomes, and was induced in the early stage of M. oryzae infection, positively regulating pathogenicity of the fungus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Yanxin Wang, Ding Li, Chaonan Dong, Yuqiang Zhao, Lei Zhang, Fan Yang, Xianfeng Ye, Yan Huang, Zhoukun Li, Zhongli Cui
Summary: In this study, a novel glycoside hydrolase family 55 beta-1,3-glucanase (AcGluA) from Archangium sp. strain AC19 was cloned and expressed in Escherichia coli, showing high activity on laminarin within a stable pH range and temperature. AcGluA hydrolyzed laminarin into a series of oligosaccharides and demonstrated significant biocontrol efficacy on rice seedlings against Magnaporthe oryzae. This study provides a new perspective for the development of green biopesticides.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zi-He Wang, Zi-Fang Shen, Jing-Yi Wang, Ying-Ying Cai, Lin Li, Jian Liao, Jian-Ping Lu, Xue-Ming Zhu, Fu-Cheng Lin, Xiao-Hong Liu
Summary: This study identified a novel calcineurin regulatory-subunit-binding protein, MoCbp7, which is involved in calcium signal transduction and is important for the growth, conidiation, appressorium formation, invasive growth, and virulence of Magnaporthe oryzae. MoCbp7 also interacts with calcineurin to regulate endoplasmic reticulum homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Ju-Hee Cha, Minsun Hong, Chang-Jun Cha
Summary: In this study, a fungal beta-glycosidase belonging to subfamily 4 of GH family 30 (GH30) was characterized with hydrolysis and transglycosylation activities towards various substrates, representing the first fungal beta-glycosidase with transglycosylation activities in subfamily 4 of GH30.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Tianle Qu, Chunyue Zhang, Zhen Qin, Liqiang Fan, Lihua Jiang, Liming Zhao
Summary: Aminooligosaccharides have various biological activities and can be widely used in food, pharmaceutical, and cosmetic industries. The cloned enzyme AoNagase showed high catalytic efficiency and good stability, making it a promising candidate for the production of aminooligosaccharides.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Mauro Mendonca, Mario Barroca, Tony Collins
Summary: This article summarizes 9 glycoside hydrolase families containing enzymes with endo-1,4-8-xylanase activity and discusses their properties, similarities, differences, and biotechnological perspectives. It provides detailed information on substrate specificities, hydrolysis patterns, and the structural determinants of these enzymes, as well as taxonomic aspects of the source organisms. The article highlights shortcomings in current knowledge and identifies research areas that require further clarification. It aims to motivate further research on these enzymes and particularly on the lesser-known endo-1,4-8-xylanase containing families, which will serve as a foundation for the knowledge-based development of process-fitted enzymes in the biobased industries of the future.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Biochemistry & Molecular Biology
Tomohiko Matsuzawa, Masahiro Watanabe, Yusuke Nakamichi, Hironaga Akita, Katsuro Yaoi
Summary: This paper reports the X-ray crystal structure of IpeA complexed with a xyloglucan oligosaccharide and reveals the critical role of Trp515 in XXXG recognition. Docking simulation suggests the involvement of Tyr268 and Tyr445 in the catalytic mechanism of IpeA.
Article
Soil Science
Limin Fan, Fajun Li, Xi Chen, Lihong Shen, Yuyao Chu, Liping Qiu, Gengdong Hu, Chao Song, Dandan Li, Shunlong Meng, Jiazhang Chen
Summary: In this study, researchers investigated the effects of the red swamp crayfish on the glycoside hydrolase families and fungal communities in soils. They found that the introduction of crayfish was the main factor responsible for the variations in GH families and fungi. Furthermore, they discovered that the abundance of fungi in soils influenced the bacterial chitinase gene abundance, rather than the crayfish exoskeleton.
APPLIED SOIL ECOLOGY
(2023)
Article
Plant Sciences
Yingmin Qu, Jing Wang, Pengyun Huang, Xiaohong Liu, Jianping Lu, Fu-Cheng Lin
Summary: Pyricularia oryzae, a plant pathogenic fungus, damages rice and wheat crops through appressorium-led tissue invasion, regulated by cAMP-PKA and Pmk1 MAPK pathways. PoRal2 plays crucial roles in fungal development and virulence by influencing appressorium formation.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Kailun Lu, Rangrang Chen, Yi Yang, Hui Xu, Jihong Jiang, Lianwei Li
Summary: The fungal cell wall plays a crucial role in regulating cellular stability and protection. The cell wall-integrity pathway is a primary signaling cascade responsible for maintaining cell wall structure and function. This pathway is associated with pathogenicity and collaborates with other signaling pathways to regulate cellular morphogenesis and secondary metabolism.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
M. Ikegaya, T. Miyazaki, E. Y. Park
Summary: The study investigates the protein BmNag31 acquired by horizontal gene transfer in the domestic silkworm, revealing its expression pattern during different developmental stages, enzymatic activity, and structural features. The findings provide insights into the function of lepidopteran GH31 alpha-N-acetylgalactosaminidase.
INSECT MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kyungyong Seong, Ksenia V. Krasileva
Summary: Structural biology has the potential to illuminate the evolution of pathogen effectors and their commonalities, with recent breakthroughs in protein structure modeling enabling genome-wide computational approaches. In this study, structure prediction methods were applied to the secretome of the fungal pathogen Magnaporthe oryzae, revealing new members of structurally similar effectors and effector families. Additionally, the evolution of putative ADP-ribose transferases with predicted structures was investigated, suggesting new evolutionary trajectories for specialized protein binders.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Plant Sciences
Jiaying Zou, Chunquan Jiang, Shanshan Qiu, Guohua Duan, Guanqun Wang, Dayong Li, Siwen Yu, Dan Zhao, Wenxian Sun
Summary: Rice false smut caused by Ustilaginoidea virens poses a significant threat to rice grain yield and quality globally. The GH42 protein UvGHF1, secreted by U. virens, not only acts as a crucial virulence factor during the infection process but also functions as a PAMP in rice and tobacco. Additionally, UvGHF1 induces cell death in tobacco and triggers pattern-triggered immunity in rice.
MOLECULAR PLANT PATHOLOGY
(2023)