期刊
MOLECULAR PLANT-MICROBE INTERACTIONS
卷 25, 期 11, 页码 1419-1429出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI-06-12-0155-R
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资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Precursory Research for Embryonic Science and Technology from Japan Science and Technology Agency
The rough lemon pathotype of Alternaria alternata produces host-selective ACR-toxin and causes Alternaria leaf spot disease of rough lemon (Citrus jambhiri). The structure of ACR-toxin I (MW = 496) consists of a polyketide with an alpha-dihydropyrone ring in a 19-carbon polyalcohol. Genes responsible for toxin production were localized to a 1.5-Mb chromosome in the genome of the rough lemon pathotype. Sequence analysis of this chromosome revealed an 8,338-bp open reading frame, ACRTS2, that was present only in the genomes of ACR-toxin-producing isolates. ACRTS2 is predicted to encode a putative polyketide synthase of 2,513 amino acids and belongs to the fungal reducing type I polyketide synthases. Typical polyketide functional domains were identified in the predicted amino acid sequence, including beta-ketoacyl synthase, acyl transferase, methyl transferase, dehydratase, beta-ketoreductase, and phosphopantetheine attachment site domains. Combined use of homologous recombination-mediated gene disruption and RNA silencing allowed examination of the functional role of multiple paralogs in ACR-toxin production. ACRTS2 was found to be essential for ACR-toxin production and pathogenicity of the rough lemon pathotype of A. alternata.
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