Review
Agriculture, Multidisciplinary
Hai-feng Zhang, Tofazzal Islam, Wen-de Liu
Summary: This article introduces and summarizes the classification, geographical distribution, host range, disease symptoms, biology and ecology, economic impact, and integrated pest management (IPM) program of rice and wheat blast diseases.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Microbiology
Jessie Fernandez, Victor Lopez, Lisa Kinch, Mariel A. Pfeifer, Hillery Gray, Nalleli Garcia, Nick Grishin, Chang-Hyun Khang, Kim Orth
Summary: The study identified two metalloproteinase enzymes, MoMca1 and MoMca2, in M. oryzae, which play significant roles in fungal growth and development, potentially regulating stress responses. The absence of these enzymes resulted in delays in fungal morphogenesis and insights into the mechanisms of M. oryzae pathogenicity.
Article
Microbiology
Pengyun Huang, Huijuan Cao, Yan Li, Siyi Zhu, Jing Wang, Qing Wang, Xiaohong Liu, Fu-Cheng Lin, Jianping Lu
Summary: This study reveals the functional diversity and complexity of melanin in different strains of Magnaporthe oryzae. Melanin promotes spore production in the rice blast pathogen, but its effect is related to the background melanin content of the wild-type strains. Transcription factors like Cnf1 have opposite effects on conidiophore differentiation in different strains. Pig1 regulates melanin synthesis in hyphae but not in conidiophores, spores, or appressoria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agronomy
Ferran Sanchez-Sanuy, Roberto Mateluna-Cuadra, Keisuke Tomita, Kazunori Okada, Gian Attilio Sacchi, Sonia Campo, Blanca San Segundo
Summary: Exposure to high iron enhances resistance to Magnaporthe oryzae infection in rice plants, possibly through upregulating defense-related genes and increasing production of phytoalexins. The infection also modulates the expression of genes involved in iron homeostasis. Iron and reactive oxygen species colocalize at infection sites. Understanding the crosstalk between iron signaling and immune signaling holds great potential for improving blast resistance in rice.
Article
Multidisciplinary Sciences
Alejandra Martinez-D'Alto, Xia Yan, Tyler C. Detomasi, Richard I. Sayler, William C. Thomas, Nicholas J. Talbot, Michael A. Marletta
Summary: Blast disease caused by the fungus Magnaporthe oryzae leads to significant crop loss in cereal plants. The study shows that the expression of the putative polysaccharide monooxygenase MoPMO9A is increased during infection. MoPMO9A exhibits activity on cereal-derived mixed β-D-glucans and its domain architecture is different from other characterized AA9 PMOs. The results suggest that MoPMO9A plays a role in MBG degradation during plant infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Dewei Yang, Shengping Li, Yueping Xiao, Ling Lu, Zichao Zheng, Dingzhong Tang, Haitao Cui
Summary: The study highlights the importance of rapid, high-amplitude transcriptional reprogramming in rice defense against blast fungus, with ribosome- and protein translation-related genes being significantly enriched among differentially expressed genes at 12 hpi in both susceptible and resistant cultivars. A core set of genes involved in response to both biotic and abiotic stress was identified, including metallothionein genes positively regulating rice resistance and a peroxidase gene negatively regulating it. This study provides novel insights into transcriptional reprogramming and serves as a valuable resource for functional studies on rice immune signaling components in resistance to blast disease.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Microbiology
Lihong Dong, Yuming Ma, Cheng-Yen Chen, Lizheng Shen, Wenda Sun, Guobing Cui, Naweed I. Naqvi, Yi Zhen Deng
Summary: The rice blast fungus Magnaporthe oryzae produces the phytohormone auxin/IAA, which is crucial for its mycelial growth and pathogenic development. This study identified an indole-3-pyruvic acid (IPA)-dependent IAA synthesis pathway in M. oryzae, consisting of the enzymes tryptophan aminotransferase (MoTam1) and indole-3-pyruvate decarboxylase (MoIpd1).
Article
Agronomy
Ammarah Shabbir, Wajjiha Batool, Dan Yu, Lili Lin, Qiuli An, Chen Xiaomin, Hengyuan Guo, Shuangshuang Yuan, Sekete Malota, Zonghua Wang, Justice Norvienyeku
Summary: This study characterized the biological function of endoxylanase I genes in the development of the rice blast fungus M. oryzae. MoXYL1A was found to be important for the virulence of M. oryzae, while MoXYL1B had a critical function in asexual reproduction of the fungus.
Article
Ecology
Kuleshwar Prasad Sahu, Aundy Kumar, Asharani Patel, Mukesh Kumar, S. Gopalakrishnan, G. Prakash, R. Rathour, Robin Gogoi
Summary: Dark brown necrotic lesions caused by Magnaporthe oryzae on rice foliage provide a contrasting microhabitat for leaf-colonizing microbiome, with 17 bacterial species identified in these lesions showing biocontrol potential against the pathogen. Over 50% of the bacterial isolates were able to suppress the growth of M. oryzae, with certain strains triggering the expression of defense genes in rice, indicating their potential as biocontrol agents for managing rice blast disease.
Article
Plant Sciences
Yingmin Qu, Jing Wang, Pengyun Huang, Xiaohong Liu, Jianping Lu, Fu-Cheng Lin
Summary: Pyricularia oryzae, a plant pathogenic fungus, damages rice and wheat crops through appressorium-led tissue invasion, regulated by cAMP-PKA and Pmk1 MAPK pathways. PoRal2 plays crucial roles in fungal development and virulence by influencing appressorium formation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Pengyun Huang, Yan Li, Jing Wang, Qing Wang, Zhicheng Huang, Xiaohong Liu, Fucheng Lin, Jianping Lu
Summary: A transcription factor, Pcf1, was found to be inhibited and degraded in nuclei of incipient appressoria in Magnaporthe oryzae, interacting with CK2 and undergoing ubiquitination in hyphae. These findings provide insight into the degradation of TFs during appressorium formation.
Article
Biochemistry & Molecular Biology
Shang Gao, Yan Hou, Qiwei Huang, Pengzhi Wu, Zhikai Han, Danhong Wei, Huabin Xie, Fengwei Gu, Chun Chen, Jiafeng Wang
Summary: In this study, the important role of Osa-miR11117 in regulating rice defense mechanisms was investigated. It was found that Osa-miR11117 targets OsPAO4 to regulate blast resistance by adjusting polyamine metabolism and ethylene signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chengyu Liu, Ningning Shen, Qian Zhang, Minghui Qin, Tingyan Cao, Shuai Zhu, Dingzhong Tang, Libo Han
Summary: This study identified a transcription factor called MoBZIP3 that plays a crucial role in the pathogenesis of the devastating fungus Magnaporthe oryzae. MoBZIP3 regulates the formation of specialized infection structure appressorium by controlling the utilization of glycogen and lipid droplets for glycerol generation. It also regulates the expression of genes related to glycerol synthesis critical for appressorium turgor pressure generation. Additionally, MoBZIP3 is involved in the regulation of multiple signaling pathways including the turgor sensor gene and actin assembly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Na Ning, Xin Xie, Haiyue Yu, Jie Mei, Qianqian Li, Shimin Zuo, Hanxiang Wu, Wende Liu, Zhiqiang Li
Summary: In this study, we identified MoPtep1 as an important pathogenic effector in rice blast. It localized in plant peroxisomes, and was induced in the early stage of M. oryzae infection, positively regulating pathogenicity of the fungus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Takayuki Motoyama, Choong-Soo Yun, Hiroyuki Osada
Summary: Filamentous fungi have numerous secondary metabolism genes producing a wide variety of secondary metabolites with unclear roles. Among them, Pyricularia oryzae, a plant pathogenic fungus, has a large number of secondary metabolism genes but only four groups have been well characterized. Activation of secondary metabolism has identified the biosynthetic genes for some of these secondary metabolites.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Editorial Material
Plant Sciences
Ji Ma, Xiong Xiao, Xingyu Wang, Min Guo
Article
Microbiology
Min Qiu, Yaning Li, Xin Zhang, Mingrun Xuan, Baiyu Zhang, Wenwu Ye, Xiaobo Zheng, Francine Govers, Yuanchao Wang
Article
Plant Sciences
Long-Jiao Hu, Xiao-Qin Wu, Hai-Yang Li, Yuan-Chao Wang, Xin Huang, Yan Wang, Yu Li
MOLECULAR PLANT PATHOLOGY
(2020)
Editorial Material
Plant Sciences
Ji Ma, Xiong Xiao, Xingyu Wang, Min Guo
Editorial Material
Plant Sciences
Ji Ma, Xiong Xiao, Xingyu Wang, Min Guo
Article
Plant Sciences
Min Qiu, Yaning Li, Wenwu Ye, Xiaobo Zheng, Yuanchao Wang
Summary: A CRISPR/Cas9-mediated in situ complementation system was developed for accurate restoration of mutated genes in Phytophthora sojae, demonstrating the potential for accelerating functional genomics research in oomycetes.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chengcheng Zheng, Weiwei Zhang, Shulin Zhang, Guogen Yang, Leyong Tan, Min Guo
Summary: This study identified the important role of Momyo1 in fungal growth, cell wall integrity, endocytosis, and virulence of Magnaporthe oryzae. Functional characterization through RNA interference showed significant effects of Momyo1 on vegetative growth, formation of appressorium-like structures, cell wall integrity, and endocytosis in the fungus. Additionally, Momyo1 was found to physically interact with MoShe4, potentially involved in endocytosis and actin cytoskeleton polarization.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Hui Feng, Chuanxu Wan, Zhichao Zhang, Han Chen, Zhipeng Li, Haibin Jiang, Maozhu Yin, Suomeng Dong, Daolong Dou, Yuanchao Wang, Xiaobo Zheng, Wenwu Ye
Summary: This study introduces a CRISPR/Cas9 system for gene manipulation in the oomycete pathogen Pythium ultimum, revealing the regulatory role of the RNA-binding protein PuM90 in oospore formation. The specific interaction between PuM90 and the 3'-UTR of PuFLP mRNA is critical for sexual reproduction in P. ultimum.
Review
Microbiology
Yan Wang, Rory N. Pruitt, Thorsten Nuernberger, Yuanchao Wang
Summary: Plant pathogens utilize various strategies to evade plant immunity, hindering the effective use of host genetic resistance for disease control. This Review discusses current knowledge on how microbial pathogens successfully infect plants by evading their immune responses and explores the potential for engineering crop resistance based on this knowledge.
NATURE REVIEWS MICROBIOLOGY
(2022)
Article
Agronomy
Shiyi Liu, Ji Ma, Bingxin Jiang, Guogen Yang, Min Guo
Summary: Rice blast fungus Magnaporthe oryzae shows a medium to high resistance risk to pydiflumetofen, with a point mutation in the MoSdhB gene responsible for the resistance. This mutation results in a replacement of histidine 245 by tyrosine, leading to a strong resistant phenotype in the fungus.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Lan Wang, Hanmei Liu, Mingmei Zhang, Yu Ye, Lei Wang, Jinyi Zhu, Zhaodan Chen, Xiaobo Zheng, Yan Wang, Yuanchao Wang
Summary: Plant pattern recognition receptors (PRRs) are receptors that sense microbial invasion and activate immune responses. This study identified a glycoside hydrolase family 12 (GH12) protein Ps109281 secreted by Phytophthora sojae during infection. Ps109281 promotes P. sojae infection but does not induce cell death. It triggers immune responses by interacting with the receptor-like protein RXEG1. The loss of cell death induction is linked to a sequence polymorphism at the N-terminus.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yao Zhao, Bo Yang, Huawei Xu, Jinbin Wu, Zhiyang Xu, Yuanchao Wang
Summary: The oomycete pathogen Phytophthora sojae secretes RXLR effectors to suppress host immune responses, promoting infection. The virulence effector Avh94 stabilizes the repressor JAZ1/2 of the jasmonic acid (JA) signaling pathway to inhibit JA signaling, thereby enhancing infection.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Han Chen, Yujie Fang, Wenrui Song, Haidong Shu, Xi Li, Wenwu Ye, Yuanchao Wang, Suomeng Dong
Summary: In this study, a highly induced PsKMT3 gene was found to play an important role in regulating reproductive development and pathogenicity in Phytophthora sojae. The deletion of PsKMT3 resulted in asexual development and pathogenicity defects. The study also revealed that histone H3K36 trimethylation was significantly reduced and involved in the activation of RxLR effector genes.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Shuchen Wang, Sen Yang, Kaixin Dai, Wenyue Zheng, Xiaoyi Zhang, Bo Yang, Wenwu Ye, Xiaobo Zheng, Yuanchao Wang
Summary: In this study, a putative effector protein Fg62 was identified from the secretome of the plant pathogenic fungus Fusarium graminearum. Fg62 modulates host immunity and facilitates infection by upregulating during early stages of infection and inducing plant cell death. It also activates plant immunity and enhances plant resistance to various pathogens.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Lin Liu, Yeqiang Xia, Yingchen Li, Yong Zhou, Xiaofeng Su, Xiaojing Yan, Yan Wang, Wende Liu, Hongmei Cheng, Yuanchao Wang, Qing Yang
Summary: Phytopathogenic fungi secrete chitin deacetylase (CDA) to evade the host's immune defense, and the authors identify common structural features of phytopathogenic fungal CDAs and find potential CDA inhibitors for controlling plant diseases.
NATURE COMMUNICATIONS
(2023)