期刊
MOLECULAR PLANT-MICROBE INTERACTIONS
卷 23, 期 12, 页码 1563-1572出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI-03-10-0051
关键词
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资金
- Ministry of Education Culture Sports Science Technology [19380029, 21380031]
- Grants-in-Aid for Scientific Research [19380029, 21380031] Funding Source: KAKEN
Several signal transduction pathways, including mitogen-activated protein kinase (MAPK) pathways, are Involved in appressorium development in Colletotrichum orbtculare, the causal agent of cucumber anthracnose disease In this study, CoMEKK1, a yeast MAPK kinases (MAPKK) kinase STE11 homolog, was identified as a disrupted gene in an Agrobacterium tumefaciens-mediated transformation mutant The phenotype of comekk1 disruptant was similar to that of cmk1, a Saccharomyces cerevistae Fus3/Kss1 MAPK homolog mutant Moreover, comekk1 and cmk1 mutants were sensitive to high osmotic and salinity stresses, indicating that Comekk1p/Cmk1p signal transduction is involved in stress tolerance The transformants of the wild type and the comekk1 mutant expressing a constitutively active form of the CoMEKK1 showed slower hyphal growth and abnormal appressorium formation, whereas those of the cmk1 disruptant did not A Cmk1p-green fluorescent protein (GFP) intracellular localization experiment indicated that nuclear localization of the Cmk1p-GFP fusion protein induced by salt stress was diminished in comekk1 mutants These results indicate that Comekk1p functions upstream of Cmk1p
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