Article
Biochemistry & Molecular Biology
Dongying Liu, Yantao Luo, Xiaofeng Zheng, Xinye Wang, Minxia Chou, Gehong Wei
Summary: NopP, a T3SS effector of Mesorhizobium amorphae CCNWGS0123, is secreted through T3SS-I during early infection stages and its deficiency reduces nitrogenase activity in Robinia pseudoacacia nodules. TRAPPC13 has been identified as a target protein of NopP in R. pseudoacacia roots, showing colocalization on the plasma membrane. The interaction between NopP and TRAPPC13 affects genes associated with cell wall remodeling and plant innate immunity in early infection stages.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Review
Microbiology
Anna Shaliutina-Loginova, Olivera Francetic, Pavel Dolezal
Summary: Bacteria have evolved sophisticated protein secretion nanomachines over billions of years to deliver toxins, enzymes, and proteins. The type II secretion system (T2SS) is used by Gram-negative bacteria to export folded proteins across the outer membrane. Recent findings have shown that components of the T2SS can be found in the mitochondria of certain eukaryotic lineages, suggesting the presence of a mitochondrial T2SS-derived system (miT2SS). This review discusses recent advances in the field and raises questions about the function and evolution of miT2SSs.
Review
Microbiology
Lisa Bowman, Tracy Palmer
Summary: The T7SS of Staphylococcus aureus plays a crucial role in virulence in disease models and intraspecies competition, with its genes located at the ess locus encoding multiple substrate recognition proteins. T7SS is widely conserved across staphylococci, encoding various toxin and immunity genes, while genomic islands encoding multiple immunity proteins in species lacking T7SS suggest a significant role for the secretion system in bacterial antagonism.
ANNUAL REVIEW OF MICROBIOLOGY, VOL 75, 2021
(2021)
Article
Microbiology
Sophie A. Howard, R. Christopher D. Furniss, Dora Bonini, Himani Amin, Patricia Paracuellos, David Zlotkin, Tiago R. D. Costa, Asaf Levy, Despoina A. I. Mavridou, Alain Filloux
Summary: The T6SS is a bacterial nanoscale weapon that delivers toxins into prey and plays a key role in the arms race between organisms. By using Hcp as bait, researchers identified uncharacterized toxins and investigated the role of Hcp in the T6SS mechanism.
Article
Microbiology
Joshua R. Prindle, Yibo Wang, Julian M. Rocha, Andreas Diepold, Andreas Gahlmann
Summary: Injectisome proteins in the cytosol form distinct complexes with each other prior to binding to injectisomes, and these interactions have important implications for the functional regulation of injectisomes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Joshua R. Prindle, Yibo Wang, Julian M. Rocha, Andreas Diepold, Andreas Gahlmann
Summary: This study used three-dimensional single-molecule tracking to elucidate the interaction of injectisome proteins in the cytosol and found that different cytosolic complexes of these proteins can form prior to injectisome binding, which has significant implications for the functional regulation of injectisomes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Zohreh N. Pourhassan, Haiyang Cui, Neele Muckhoff, Mehdi D. Davari, Sander H. J. Smits, Ulrich Schwaneberg, Lutz Schmitt
Summary: Protein secretion is advantageous for recombinant protein production. Type 1 secretion systems (T1SS) have a simple architecture and are attractive for biotechnological applications. The hemolysin A type 1 secretion system (HlyA T1SS) from Escherichia coli has been used for secretion of heterologous proteins, but its commercial utility is limited by low secretion titers. To improve this, we engineered the inner membrane complex of the system using KnowVolution strategy, resulting in a novel HlyB variant with improved secretion for two hydrolases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sefli Sri Wahyu Effendi, Shih- Tan, Wan-Wen Ting, I-Son Ng
Summary: The study found that co-expressing MlCA with protein partners effectively increased the solubility, activity, and turnover rate, while also affecting its stability in temperature, pH range, and presence of metal ions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xiaoye Liang, Tong-Tong Pei, Zeng-Hang Wang, Weiliang Xiong, Li-Li Wu, Ping Xu, Shuangjun Lin, Tao G. Dong
Summary: The Type VI Secretion System (T6SS) is utilized by Gram-negative bacteria for interspecies interaction. This study identified a lysozyme-like effector TseP in Aeromonas dhakensis, showcasing its cell wall-lysing activities and T6SS dependency. Inactivating TseP and two other effectors led to the creation of a detoxified T6SS-active strain for potential protein delivery to various recipient cells.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
B. Izquierdo Lafuente, R. Ummels, C. Kuijl, W. Bitter, A. Speer
Summary: CpnT, a potent secreted toxin of Mycobacterium tuberculosis, is exclusively secreted by the ESX-5 system in bacterial culture but relies on intact ESX-1, ESX-4, and ESX-5 systems during infection studies. This highlights the intricate interplay of three different secretion systems in secreting one substrate during infection.
Article
Engineering, Chemical
Wan-Wen Ting, Sefli Sri Wahyu Effendi, Ruei-En Hu, I-Son Ng
Summary: The study aims to enhance protein production and catalytic efficiency of MlCA through directed evolution in genetically Escherichia coli. The CRISPRi-based DEPEND-2.0 system effectively inhibits cell growth, and structural prediction and codon design improve enzyme activity and protein expression. The developed MlCA variant shows high durability and catalytic activity.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2023)
Article
Physiology
Olivia Spitz, Isabelle N. Erenburg, Kerstin Kanonenberg, Sandra Peherstorfer, Michael H. H. Lenders, Jens Reiners, Miao Ma, Ben F. Luisi, Sander H. J. Smits, Lutz Schmitt
Summary: The toxin hemolysin A is secreted by a dedicated secretion machinery in uropathogenic E. coli strains. This machinery, consisting of TolC, HlyD and HlyB proteins, belongs to the Type I secretion system family. Researchers proposed an amphipathic helix in the C-terminal region of the toxin that plays a crucial role in the early steps of secretion.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Microbiology
Timothy A. Klein, Dirk W. Grebenc, Prakhar Y. Shah, Owen D. McArthur, Brandon H. Dickson, Michael G. Surette, Youngchang Kim, John C. Whitney
Summary: Type VIIb secretion systems (T7SSb) are utilized by various Gram-positive bacteria to facilitate interbacterial killing and pathogenesis through the secretion of toxins. This study discovered two new protein families, Lap1 and Lap2, that interact with these toxins and are essential for their secretion, providing new insights into T7SSb-dependent toxin release from Gram-positive bacteria.
Article
Biotechnology & Applied Microbiology
Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: In this study, the effects of different components in the growth medium on protein secretion titer were investigated. It was found that the optimal combination of carbon sources, buffers, and salts significantly increased secretion titer. This work demonstrates the importance of optimizing medium composition to enhance protein secretion titer in bacteria.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
R. Wassem, A. M. Marin, A. Daddaoua, R. A. Monteiro, L. S. Chubatsu, J. L. Ramos, W. J. Deakin, W. J. Broughton, F. O. Pedrosa, E. M. Souza
ENVIRONMENTAL MICROBIOLOGY
(2017)
Article
Microbiology
Jeremie Gay-Fraret, Silvia Ardissone, Kumiko Kambara, William J. Broughton, William J. Deakin, Antoine Le Quere
FEMS MICROBIOLOGY LETTERS
(2012)
Article
Microbiology
Silvia Ardissone, Hajime Kobayashi, Kumiko Kambara, Coralie Rummel, K. Dale Noel, Graham C. Walker, William J. Broughton, William J. Deakin
JOURNAL OF BACTERIOLOGY
(2011)
Article
Plant Sciences
Olivier Schumpp, Michele Crevecoeur, William J. Broughton, William J. Deakin
JOURNAL OF EXPERIMENTAL BOTANY
(2009)
Article
Microbiology
Samanta Bolzan de Campos, William J. Deakin, William J. Broughton, Luciane M. P. Passaglia
Article
Biochemistry & Molecular Biology
Roseli Wassem, Hajime Kobayashi, Kumiko Kambara, Antoine Le Quere, Graham C. Walker, William J. Broughton, William J. Deakin
MOLECULAR MICROBIOLOGY
(2008)
Article
Biochemistry & Molecular Biology
Kumiko Kambara, Silvia Ardissone, Hajime Kobayashi, Maged M. Saad, Olivier Schumpp, William J. Broughton, William J. Deakin
MOLECULAR MICROBIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Silvia Ardissone, K. Dale Noel, Mitchell Klement, William J. Broughton, William J. Deakin
MOLECULAR PLANT-MICROBE INTERACTIONS
(2011)
Review
Microbiology
William J. Deakin, William J. Broughton
NATURE REVIEWS MICROBIOLOGY
(2009)
Review
Plant Sciences
Olivier Schumpp, William J. Deakin
TRENDS IN PLANT SCIENCE
(2010)
Article
Plant Sciences
O. Schumpp, M. E. Ramel, P. Gugerli, W. J. Broughton, W. J. Deakin
JOURNAL OF PLANT RESEARCH
(2007)
Article
Biochemistry & Molecular Biology
Antoine J. -L. Le Quere, William J. Deakin, Christel Schmeisser, Russell W. Carlson, Wolfgang R. Streit, William J. Broughton, L. Scott Forsberg
JOURNAL OF BIOLOGICAL CHEMISTRY
(2006)
Article
Microbiology
W. J. Broughton, M. Hanin, B. Relic, J. Kopcinska, W. Golinowski, S. Simsek, T. Ojanen-Reuhs, B. Reuhs, C. Marie, H. Kobayashi, B. Bordogna, A. Le Quere, S. Jabbouri, R. Fellay, X. Perret, W. J. Deakin
JOURNAL OF BACTERIOLOGY
(2006)
Article
Microbiology
Maged M. Saad, Christian Staehelin, William J. Broughton, William J. Deakin
JOURNAL OF BACTERIOLOGY
(2008)
