Article
Biochemistry & Molecular Biology
Shiny B. Martis, Michel Droux, William Nasser, Sylvie Reverchon, Sam Meyer
Summary: This study discovered that the dynamic expression of pectate lyases in Dickeya dadantii is controlled by a metabolic switch involving glucose and pectin. The researchers measured the concentration profiles of key metabolites and developed a dynamic model to explain the process. The model showed that the activity of pelE and pelD genes is regulated by a mechanism of carbon catabolite repression, directly affecting the virulence of D. dadantii.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Przepiora, Donata Figaj, Aleksandra Bogucka, Jakub Fikowicz-Krosko, Robert Czajkowski, Nicole Hugouvieux-Cotte-Pattat, Joanna Skorko-Glonek
Summary: DsbA oxidoreductase plays a crucial role in introducing disulfide bonds in bacteria, and its absence may result in the loss of virulence. In this study, a D. solani dsbA mutant was constructed, and it was found that the lack of DsbA led to the loss of virulence and affected multiple aspects of bacterial physiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Clara Galvez-Roldan, Jean Paul Cerna-Vargas, Jose Juan Rodriguez-Herva, Tino Krell, Saray Santamaria-Hernando, Emilia Lopez-Solanilla
Summary: Nitrate metabolism is important in bacterial physiology. The nitrate- and nitrite-sensing (NIT) domain-containing chemoreceptor of Dickeya dadantii 3937 (Dd3937) plays a key role in plant pathogenic bacteria's entry and infection success. Nitrate perception not only affects the expression of nitrate reductase genes but also controls the expression of virulence determinants in Dd3937.
Article
Microbiology
Simon Leonard, Camille Villard, William Nasser, Sylvie Reverchon, Florence Hommais
Summary: The RNA chaperones Hfq and ProQ play important roles in regulating pathogenicity in Dickeya dadantii, with mutants showing reduced virulence functions such as decreased ability to cause soft rot on host leaves. Their effects are likely mediated through post-transcriptional regulation of virulence genes by non-coding RNAs.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maiwenn Pineau, Shiny B. Martis, Raphael Forquet, Jessica Baude, Camille Villard, Lucie Grand, Florence Popowycz, Laurent Soulere, Florence Hommais, William Nasser, Sylvie Reverchon, Sam Meyer
Summary: DNA supercoiling is a crucial mechanism in bacterial chromosome compaction, which is regulated by specific topoisomerases, physiological conditions, and genomic context. The altered supercoiling level leads to changes in global gene expression in bacteria.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Tyler C. Helmann, Melanie J. Filiatrault, Paul V. Stodghill
Summary: In this study, the gene fitness of model and non-model Dickeya strains under different conditions was compared, revealing shared and variable traits important for potato soft rot disease pathogenicity. The study found that bacterial growth in minimal medium and potato tubers required various metabolic traits and the pectin degradation gene. Disruption in certain DNA-binding proteins also had strain-specific effects on competitive fitness. However, the impact of strain-level variation on virulence remains to be further studied.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiujuan Zhou, Bin Liu, Yanhong Liu, Chunlei Shi, Pina M. Fratamico, Lida Zhang, Dapeng Wang, Jianhua Zhang, Yan Cui, Ping Xu, Xianming Shi
Summary: The deletion of genes SC0368 and SC0595 in Salmonella Choleraesuis led to defects in motility and increased invasiveness in Caco-2 cells. Additionally, SC0368 deletion resulted in up-regulation of genes encoding cytochromes, enhancing bacterial proliferation. These findings suggest that these two genes play important roles in flagella biosynthesis, LPS synthesis, bacterial growth, and virulence within Salmonella C1 serogroup.
Article
Multidisciplinary Sciences
Martine Rigault, Sylvie Citerne, Celine Masclaux-Daubresse, Alia Dellagi
Summary: Autophagy plays important roles in plant immunity, affecting the susceptibility of plants to pathogens. Mutations in genes related to autophagy can lead to increased susceptibility, mainly due to disruptions in hormonal balance.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Wanqin He, Deqing Huang, Jiayu Wu, Xue Li, Yajuan Qian, Bin Li, Binggan Lou, Jianxiang Wu
Summary: This study developed four highly specific and sensitive monoclonal antibodies (MAbs) for detection of Dickeya dadantii, a bacterial pathogen causing sweet potato stem and root rot. These MAbs showed efficient detection capabilities through indirect enzyme-linked immunosorbent assay (indirect-ELISA) and were successfully utilized in three different serological approaches for detecting the pathogen in field-collected sweet potato plants. These new serological methods were more sensitive than conventional PCR and proved to be reliable for diagnosis of stem and root rot in sweet potato production.
Article
Biochemistry & Molecular Biology
Melissa C. Schofield, Daniela Q. Rodriguez, Amanda A. Kidman, Erin K. Cassin, Lia A. Michaels, Elizabeth A. Campbell, Peter A. Jorth, Boo Shan Tseng
Summary: In infections of cystic fibrosis airways, the gene mucA in Pseudomonas aeruginosa is crucial for bacterial viability; mutations in mucA can result in misregulation of AlgU and affect bacterial phenotype and disease outcomes.
MOLECULAR MICROBIOLOGY
(2021)
Article
Plant Sciences
Bo Wang, Zhizhou Xu, Yangyang Zhao, Guichun Wu, Kaihuai Li, Rongxian Hou, Baodian Guo, Bao Tang, Yancun Zhao, Fengquan Liu
Summary: Avoiding host defence system is crucial for the survival of pathogens. This study identified a key SFN tolerance-related gene, saxF, in Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot. Furthermore, the study discovered that the transcription of saxF is regulated by a novel transcription factor, SstF, which can sense SFN and activate the expression of saxF to increase SFN resistance. These findings provide insights into the mechanism by which Xcc senses host defence signals and enhances its virulence.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Microbiology
Hidetada Hirakawa, Mizuki Shimokawa, Koshi Noguchi, Minori Tago, Hiroshi Matsuda, Ayako Takita, Kazutomo Suzue, Hirotaka Tajima, Ikuro Kawagishi, Haruyoshi Tomita
Summary: In this study, it was found that TosR is an activator of flagellar expression in Uropathogenic Escherichia coli (UPEC). Loss of TosR gene reduces the motility and aggregation ability of UPEC in urothelial cells.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Zeqiong Cai, Fan Yang, Xiaolong Shao, Zhuo Yue, Zhenpeng Li, Yuqin Song, Xiaolei Pan, Yongxin Jin, Zhihui Cheng, Un-Hwan Ha, Jie Feng, Liang Yang, Xin Deng, Weihui Wu, Fang Bai
Summary: The ECF sigma factor HxuI in Pseudomonas aeruginosa is rapidly induced in response to host stresses and activates multiple metabolic and virulence pathways, promoting bacterial colonization and pathogenicity in host infections. The findings of this study demonstrate the importance of HxuI in the adaptability and virulence of P. aeruginosa during infection.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Feng He, Alex-Machio Kange, Jie Yang, Jiaxin Xiao, Rongbo Wang, Lu Yang, Yifan Jia, Zheng Qing Fu, Yancun Zhao, Fengquan Liu
Summary: In this study, the researchers characterized a transcription factor called VpxlnR and investigated its role in the growth, development, stress response, and virulence of the fungal pathogen Valsa pyri. They found that VpxlnR controls genes involved in signaling pathways and extracellular enzyme activities, and its mutants showed reduced growth, fruiting body formation, and virulence.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Jakub Drzmisek, Denisa Petrackova, Ana Dienstbier, Ivana Curnova, Branislav Vecerek
Summary: Bordetella pertussis produces the type III secretion system, and the novel chaperone BP2265 interacts specifically with the secreted T3SS regulator BtrA. In the absence of BP2265, secretion of BtrA and most T3SS substrates is severely impaired, while their production remains unaffected.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Plant Sciences
George W. Sundin, Nian Wang, Amy O. Charkowski, Luisa F. Castiblanco, Hongge Jia, Youfu Zhao
Article
Agronomy
Yan Wang, Amy O. Charkowski, Cuiyun Zeng, Tiantian Zhu, Huizhen Wang, Honggang Chen
Review
Plant Sciences
Amy O. Charkowski
ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 56
(2018)
Article
Plant Sciences
J. J. Couture, A. Singh, A. O. Charkowski, R. L. Groves, S. M. Gray, P. C. Bethke, P. A. Townsend
Article
Agronomy
Chakradhar Mattupalli, Jeremy D. Glasner, Amy O. Charkowski
AMERICAN JOURNAL OF POTATO RESEARCH
(2014)
Article
Agronomy
Brooke N. Weber, R. Andrews Witherell, Amy O. Charkowski
AMERICAN JOURNAL OF POTATO RESEARCH
(2015)
Article
Biotechnology & Applied Microbiology
Lavanya Babujee, Jennifer Apodaca, Venkatesh Balakrishnan, Paul Liss, Patricia J. Kiley, Amy O. Charkowski, Jeremy D. Glasner, Nicole T. Perna
Article
Biotechnology & Applied Microbiology
Bing Ma, Amy O. Charkowski, Jeremy D. Glasner, Nicole T. Perna
Article
Plant Sciences
Kenneth E. Frost, Russell L. Groves, Amy O. Charkowski
Article
Plant Sciences
Yuan Zeng, Ana Cristina Fulladolsa, Andrew Houser, Amy O. Charkowski
Article
Multidisciplinary Sciences
Clifford S. Hogan, Beth M. Mole, Sarah R. Grant, David K. Willis, Amy O. Charkowski
Article
Microbiology
Grace Kwan, Amy O. Charkowski, Jeri D. Barak
Article
Biochemical Research Methods
Lavanya Babujee, Robert A. Witherell, Kakeru Mikami, Daigo Aiuchi, Amy O. Charkowski, Aurelie M. Rakotondrafara
JOURNAL OF VIROLOGICAL METHODS
(2019)
Article
Microbiology
Xing Ma, Nicole T. Perna, Jeremy D. Glasner, Jianjun Hao, Steven Johnson, Afnan Shazwan Nasaruddin, Amy O. Charkowski, Shan Wu, Zhangjun Fei, Keith L. Perry, Paul Stodghill, Bryan Swingle
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Review
Agronomy
Sarah Braun, Amanda Gevens, Amy Charkowski, Christina Allen, Shelley Jansky
AMERICAN JOURNAL OF POTATO RESEARCH
(2017)