Article
Biotechnology & Applied Microbiology
Keiko Kobayashi, Kae Akita, Masashi Suzuki, Daisaku Ohta, Noriko Nagata
Summary: The exine and pollen coat of pollen grains play vital roles in protecting and facilitating the fertilization process. Mutants lacking one or the other component can exhibit different fertility phenotypes, highlighting the importance of both structures for successful reproduction.
PLANT BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Ting Zou, Pingping Xiong, Fuxing Zhou, Dan Zhou, Hao Chen, Gongwen Li, Kun Peng, Kaiyou Zheng, Yuhao Han, Kaixuan Zhang, Xu Zhang, Shangyu Yang, Qiming Deng, Shiquan Wang, Jun Zhu, Yueyang Liang, Changhui Sun, Xiumei Yu, Huainian Liu, Lingxia Wang, Ping Li, Shuangcheng Li
Summary: This study identifies AMD1 as an important regulatory component in rice pollen development, involved in tapetum development and pollen exine patterning. AMD1 directly activates the transcription of genes related to sporopollenin biosynthesis and interacts with key transcription factors. This research contributes to a better understanding of the regulatory network in rice sexual reproduction and provides a potential genetic engineering target for hybrid rice breeding.
Article
Biochemistry & Molecular Biology
Masahiro M. Kanaoka, Kentaro K. Shimizu, Bo Xie, Sinisa Urban, Matthew Freeman, Zonglie Hong, Kiyotaka Okada
Summary: Fertilization is a key event in sexually reproducing plants. A study on Arabidopsis thaliana identified a mutant, kompeito-1 (kom-1), which showed defects in pollen wall patterning and pollen-stigma adhesion. The study found that the KOM gene is involved in the regulation of CalS5 protein accumulation, which in turn affects callose accumulation and pollen wall formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Riaz Muhammad Khan, Ping Yu, Lianping Sun, Adil Abbas, Liaqat Shah, Xiaojiao Xiang, Dongfei Wang, Amir Sohail, Yingxin Zhang, Qunen Liu, Shihua Cheng, Liyong Cao
Summary: In angiosperms, anther development involves complex biological processes crucial for pollen viability. In rice, various genes have been identified to play roles in callose regulation and pollen wall patterning, although pollen development mechanisms remain unclear. The characterization of the rice mutant dcet1 sheds light on callose regulation, pollen wall patterning, and tapetal cell PCD, contributing to our understanding of rice male reproductive development and its potential applications in hybrid rice breeding.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Jinlin Feng, Minghui Qin, Lixia Yao, Yan Li, Rong Han, Ligeng Ma
Summary: Naa50 plays a crucial role in balancing the degradation of tapetum cells during anther development and is important for pollen development by affecting multiple pathways.
Article
Plant Sciences
Liping Shen, Feng Tian, Zhukuan Cheng, Qiang Zhao, Qi Feng, Yan Zhao, Bin Han, Yuhan Fang, Yanan Lin, Rui Chen, Donghui Wang, Wenfeng Sun, Jiaqi Sun, Hongyun Zeng, Nan Yao, Ge Gao, Jingchu Luo, Zhihong Xu, Shunong Bai
Summary: This study reveals the important role of OsMADS58 in stamen development in rice by analyzing a CRISPR knockout mutant. It suggests that OsMADS58 may exert pleiotropic effects on stamen development by stabilizing gene regulatory circuits during early stages.
Article
Plant Sciences
Tzu-Hsiang Huang, Der-Fen Suen
Summary: Iron deficiency in floral buds can lead to impaired pollen development. Supplying floral buds with iron can restore the deficiency and improve pollen development. Gene expression changes in iron-deficient anthers affect tapetum function and pollen development.
Article
Plant Sciences
Rui Wang, Heather A. Owen, Anna A. Dobritsa
Summary: Research using electron microscopy on developing Arabidopsis pollen revealed the complex ultrastructure of PE during the tetrad stage, with significant changes in carbohydrate components observed at different stages. PE appears to be a dynamic structure rather than static, playing a critical role in forming reticulate exine of pollen walls.
Review
Cell Biology
Shuaijie Wei, Ligeng Ma
Summary: In flowering plants, pollen development is crucial for sexual reproduction. Studies have shown that both gametophytic and sporophytic factors coordinate the regulation of pollen development. The tapetum, a somatic cell layer adjacent to the developing male meiocytes, plays a vital role in providing essential materials for pollen wall formation. Disruption in tapetum specification, development, or function leads to defects in pollen development. This review aims to summarize the current understanding of tapetum-mediated pollen development and its molecular mechanism in Arabidopsis thaliana.
Article
Plant Sciences
Jie Liu, Roberta Ghelli, Maura Cardarelli, Markus Geisler
Summary: In this study, the essential role of FKBP42, TWISTED DWARF1 (TWD1) in the regulation of stamen elongation through ABCB-mediated auxin transport is demonstrated. TWD1 promotes stamen elongation, anther dehiscence, and pollen maturation, and is required for seed development. ABCB1 and ABCB19 appear to have different functions during different stages of stamen development.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Kaiqi Wang, Xin Zhao, Chaoting Pang, Sida Zhou, Xuexue Qian, Nan Tang, Naiying Yang, Ping Xu, Xiaofeng Xu, Jufang Gao
Summary: The newly discovered gene IEF, regulated by AMS, plays a crucial role in exine formation and is involved in sporopollenin transportation. Its mutation leads to severe male sterility and defective pollen development, highlighting the importance of IEF in pollen development and male fertility.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Francesca Resentini, Matteo Grenzi, Daniele Ancora, Mara Cademartori, Laura Luoni, Marianna Franco, Andrea Bassi, Maria Cristina Bonza, Alex Costa
Summary: The study utilizes genetically encoded Ca2+ indicators to reveal details of Ca2+ dynamics in plant cells, including the processing time of Ca2+ from the cytosol to the ER and the time to observe cytosolic Ca2+ increase with ER inhibition. Live imaging of mature plants also shows a wounding-induced long-distance ER Ca2+ wave, enhancing the understanding of intracellular Ca2+ dynamics and signaling.
Article
Cell Biology
Karen S. Renzaglia, Neil W. Ashton, Dae-Yeon Suh
Summary: In this study, the complete process of sporogenesis in Physcomitrium patens is described, including the formation of the spore wall and aperture. Both diploid and haploid cells contribute to the development of spores. This research provides a foundation for comparing with other plant groups and for future studies on the genetics and evolution of spores.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Plant Sciences
Mei Bai, Han Gao, Yang Yang, Hong Wu
Summary: In mature pollen grains, lipids are primarily stored in lipid droplets and play a crucial role in pollen development and germination. The enzyme DGAT1 is important for triacylglycerol synthesis and its mutation leads to a decrease in TAG content and altered fatty acid composition in pollen. The expression of genes involved in TAG synthesis is downregulated in the mutant pollen as11, while starch synthesis genes are upregulated. The mutant pollen also exhibits changes in lipid droplet content and ultrastructural alterations, including a decrease in endoplasmic reticulum and increase in Golgi body content.
Article
Plant Sciences
Yunyun Song, Yongyan Tang, Lingtong Liu, Yunyuan Xu, Tai Wang
Summary: Pollen exine, composed of nexine, bacula, and tectum, is essential for pollen viability and function. This study reveals the role of PEM1 in rice pollen development and suggests that PEM1 mediates Ubisch body formation and pollen exine development by negatively modulating gene expression.
Article
Agronomy
Yu Han, Si-Da Zhou, Jiong-Jiong Fan, Lei Zhou, Qiang-Sheng Shi, Yan-Fei Zhang, Xing-Lu Liu, Xing Chen, Jun Zhu, Zhong-Nan Yang
Summary: Overall, OsMS188 is identified as a key regulator of tapetum development and pollen wall formation in rice. The gene regulatory network established in this study sheds light on future investigations of fertility regulation in rice and other crop species.
Article
Biochemistry & Molecular Biology
Kai-Qi Wang, Ya-Hui Yu, Xin-Lei Jia, Si-Da Zhou, Fang Zhang, Xin Zhao, Ming-Yue Zhai, Yi Gong, Jie-Yang Lu, Yuyi Guo, Nai-Ying Yang, Shui Wang, Xiao-Feng Xu, Zhong-Nan Yang
Summary: Understanding photoperiod/temperature-sensitive genic male sterility (P/TGMS) is important for improving crop production. This study identified a gene, res3, that can restore male sterility and investigated its mechanism of action. The findings suggest that res3 plays a role in tapetum secretory function and can compensate for pollen wall defects in other P/TGMS lines. These results provide valuable insights into the cellular mechanism of fertility restoration in P/TGMS lines.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Yue Jin, Xinyue Song, Huizhong Chang, Yueyue Zhao, Chenhao Cao, Xinbao Qiu, Jun Zhu, Ertao Wang, Zhongnan Yang, Nan Yu
Summary: Pollen protects male sperm and regulates anther development, allowing flowering plants to adapt to different terrestrial environments. The SLR1 gene in rice plays a crucial role in anther development, mainly expressed in tapetal cells and tetrads, and interacts with OsMS188 to regulate pollen wall formation.
Article
Plant Sciences
Cheng Zhang, Meng-Yi Ren, Wen-Jian Han, Ya-Fei Zhang, Min-Jia Huang, Si-Yuan Wu, Jie Huang, Yun Wang, Zheng Zhang, Zhong-Nan Yang
Summary: Slow development has been shown to restore the fertility of thermo-sensitive and photoperiod-sensitive genic male sterile lines in Arabidopsis, with RPG1 and RPG2 playing redundant roles in fertility restoration. Under slow growth conditions, primexine formation in rpg1 is partially restored, leading to fertility recovery. Additionally, RPG2 is essential for fertility restoration in rpg1, with reduced fertility observed under low temperature conditions. The study highlights the importance of gene redundancy in fertility restoration for TGMS lines and provides insights into pollen wall pattern formation.
Article
Plant Sciences
Zaibao Zhang, Huadong Zhan, Jieyang Lu, Shuangxi Xiong, Naiying Yang, Hongyu Yuan, Zhong-Nan Yang
Summary: The KCS family members expressed in anther play crucial roles in the accumulation of pollen coat lipids, particularly KCS7, KCS15, and KCS21 expressed in tapetal cells. These proteins are involved in pollen-stigma interaction by affecting the hydration and pollen tube growth, revealing the importance of tapetal-derived pollen coat lipids.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yanfei Zhang, Chao Chen, Zexin Jin, Zhongnan Yang, Yueling Li
Summary: This study investigated the effects of light intensity on the morphology, photosynthesis, chloroplast ultrastructure, and chloroplast gene expression of Heptacodium miconioides (H. miconioides). The research found that light intensity affected leaf morphology, chloroplast development, and photosynthetic efficiency.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Yilu Wang, Yinhua Lu, Jieyang Lu, Zhong-Nan Yang, Zhongyi Yang
Summary: Ethylenediamine-N,N'-disuccinic acid [(S,S)-EDDS] is a biodegradable strong chelating agent that can currently be produced by biological methods, including fermentation and enzymatic synthesis. Enzymatic methods have a clearer mechanism than fermentation and are more economical and environmentally friendly.
PROCESS BIOCHEMISTRY
(2022)
Article
Plant Sciences
Zi-Yuan Wang, Wan-Tong Qu, Tong Mei, Nan Zhang, Nai-Ying Yang, Xiao-Feng Xu, Hai-Bo Xiong, Zhong-Nan Yang, Qing-Bo Yu
Summary: This study further analyzed the function of AtRsmD in chloroplast development and photosynthesis, revealing its distinct regulatory roles in chloroplast translation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Nai-Ying Yang, Xin-Lei Jia, Chang-Xu Sui, Shi-Yi Shen, Xi-Ling Dai, Jing-Shi Xue, Zhong-Nan Yang
Summary: Reconstructing the development of sporangia in seed-free vascular plants is crucial for understanding spore production. This study used fluorescence imaging and confocal microscopy to reconstruct the key steps of sporangia development, with a focus on the cell wall structures of tapetum and spore mother cells. By staining the cell wall and cytoplasm, the researchers observed the development of sporangia in Pteris multifida and revealed the clear cell lineages involved. This work improves our overall understanding of fern spore formation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hai-Bo Xiong, Hui-Min Pan, Qiao-Ying Long, Zi-Yuan Wang, Wan-Tong Qu, Tong Mei, Nan Zhang, Xiao-Feng Xu, Zhong-Nan Yang, Qing-Bo Yu
Summary: This study reveals the mechanism of transcription-translation coupling in chloroplasts and finds that AtNusG-mediated coupling contributes to the establishment of photosynthetic capacity and response to environmental changes in plants.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Yan-Fei Zhang, Yue-Ling Li, Xiang Zhong, Jun-Jie Wang, Lei Zhou, Yu Han, Dan-Dan Li, Na Wang, Xue-Hui Huang, Jun Zhu, Zhong-Nan Yang
Summary: A novel rice TGMS line, ostms18, was identified in this study, showing more stable sterility in summer compared to the widely used tms5 locus. The TGMS trait of ostms18 is caused by a point mutation in a GMC oxidoreductase gene, resulting in defective pollen wall formation under high temperature but restoration of fertility under low temperature.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Cell Biology
Ben-Shun Zhu, Ying-Xiu Zhu, Yan-Fei Zhang, Xiang Zhong, Keng-Yu Pan, Yu Jiang, Chi-Kuang Wen, Zhong-Nan Yang, Xiaozhen Yao
Summary: Ethylene inhibits anther development through the EIN2-EIN3/EIL1 signaling pathway, leading to pollen abortion.
Article
Biochemistry & Molecular Biology
Chong Wang, Hao Cheng, Wenjing Xu, Jingshi Xue, Xinguo Hua, Guimin Tong, Xujun Ma, Chuanping Yang, Xingguo Lan, Shi-Yi Shen, Zhongnan Yang, Jirong Huang, Yuxiang Cheng
Summary: This study reveals the roles of pollen-specific GDPD-LIKE genes in pollen tube tip growth. The GDPD-LIKE genes mediate pollen tube tip growth by modulating cellulose deposition in pollen tube walls. These findings are important for understanding the mechanism of pollen tube growth and seed formation.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Yunxia He, Xiaomeng He, Xiaoyang Wang, Mengyue Hao, Jiale Gao, Yangxiayu Wang, Zhong-Nan Yang, Xiangzong Meng
Summary: EPFL4, EPFL5, and EPFL6 peptides promote stamen filament elongation by enhancing filament cell proliferation. Loss of EPFL4-6 genes results in defective filament cell proliferation, leading to pollination failure and male sterility.
Article
Biotechnology & Applied Microbiology
Yu Han, Sheng-Zhe Jiang, Xiang Zhong, Xing Chen, Chang-Kai Ma, Yan-Ming Yang, Yi-Chen Mao, Si-Da Zhou, Lei Zhou, Yan-Fei Zhang, Xue-Hui Huang, Hui Zhang, Lai-Geng Li, Jun Zhu, Zhong-Nan Yang
Summary: We reported a novel rice thermosensitive genic male sterility (TGMS) line ostms15, which is male sterile under high temperature and fertile under low temperature. Field evaluation from 2018 to 2021 revealed that ostms15 has more stable sterility under high temperature compared to tms5 (ZH11), indicating its considerable value for rice breeding. We also identified a point mutation in the OsTMS15 gene of ostms15, leading to the TGMS phenotype.
PLANT BIOTECHNOLOGY JOURNAL
(2023)