期刊
MOLECULAR PHARMACEUTICS
卷 11, 期 3, 页码 922-937出版社
AMER CHEMICAL SOC
DOI: 10.1021/mp400589q
关键词
pneumococcal capsular polysaccharides; antigen-delivery systems; polylactide nanoparticles; opsonophagocytic assay; memory antibody; pneumococcal surface antigen A (PsaA); pneumococcal surface protein A (PspA)
资金
- National Institute of Immunology (NII), New Delhi
- Department of Biotechnology, Government of India [BT/PR4411/PID/06/190/2003]
- Tata Innovation Fellowship, Department of Biotechnology, Government of India
Bacterial capsular polysaccharides are components of many modern vaccines, but they are weakly immunogenic. Herein, we describe the delivery of pneumococcal capsular polysaccharide serotype-1 (PCP-1) in polylactide polymeric particles to enhance its immunogenicity. Immunization with PCP-1-entrapped particles elicited long-term memory antibody responses from a single intramuscular injection. PCP-1-entrapped nanoparticles (NPs) elicited significantly higher anti-PCP-1 IgG responses than that observed with soluble and microparticles (MPs) formulations. Delivering PCP-1 and pneumococcal proteins in same particles did not improve the IgG response. The sera of animals immunized with PCP-1-entrapped particles promoted efficient opsonophagocytosis of pneumococci by macrophages. Single-dose immunization with PCP-1-entrapped particles conferred a long-term serotype-specific protection against lethal pneumococcal challenge. The higher immunogenicity of PCP-1 nanoparticles showed correlation with enhanced uptake by antigen-presenting cells. The results highlight the potential of polymeric nanoparticles as an efficient means of presenting polysaccharide antigens to the immune system.
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