期刊
MOLECULAR PHARMACEUTICS
卷 9, 期 9, 页码 2415-2423出版社
AMER CHEMICAL SOC
DOI: 10.1021/mp200389t
关键词
chemical cross-linking; conjugation; drug and gene delivery; fluorescein; hepatitis B virus; HBcAg; oligonucleotide packaging
资金
- FRGS [01-01-07-161FR, 02-01-04-SF1148]
- Universiti Putra Malaysia
Cell-internalizing peptides (CIPs) can be used to mediate specific delivery of nanoparticles across cellular membrane. The objective of this study was to develop a display technique using hepatitis B virus (HBV) capsid-bin ding peptide as a nanoglue to present CIPs on HBV nanoparticles for cell-targeting delivery. A CIP was selected from a phage display library and cross-linked specifically at the tips of the spikes of the HBV capsid nanoparticle via the nanoglue by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). Fluorescent oligonucleotides packaged in the nanoparticles and the fluorescein molecules conjugated on the nanoparticles were delivered to cells by using this display technique. This study demonstrated a proof of principle for cell-targeting delivery via nanoglue bioconjugation.
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