Article
Microbiology
Michael Koczerka, Pierre-Emmanuel Douarre, Florent Kempf, Sebastien Holbert, Michel-Yves Mistou, Olivier Grepinet, Isabelle Virlogeux-Payant
Summary: The study revealed the widespread presence of the rck gene in various subspecies of Salmonella, raising questions about its evolution. The results emphasize the important role of rck in virulence and contribute to understanding the role of Rck in the pathogenesis of Salmonella.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Thaisy Pacheco, Ana Erika Inacio Gomes, Nathalia Maria Goncalves Siqueira, Lucas Assoni, Michelle Darrieux, Henrietta Venter, Lucio Fabio Caldas Ferraz
Summary: The study characterizes a sdiA mutant strain of Klebsiella pneumoniae, revealing increased biofilm formation and altered cell division and fimbriae expression. The findings demonstrate that SdiA plays a role in regulating cell division, virulence factor expression, biofilm formation, and production of quorum sensing autoinducers in K. pneumoniae.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Hovakim Grabski, Siranuysh Ginosyan, Susanna Tiratsuyan
Summary: Enteropathogenic Escherichia coli is a significant pathogen causing persistent diarrhea in children worldwide. The bacteria can form biofilms, and its mechanisms, including virulence factors and biofilm formation, are regulated by quorum sensing. SdiA receptor in enteropathogenic E. coli can detect autoinducers and may serve as a potential target for anti-infectious techniques.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Mohammad Z. Ahmed, Ghazala Muteeb, Saif Khan, Ali S. Alqahtani, Pallavi Somvanshi, Mohammed S. Alqahtani, Keshav Lalit Ameta, Shafiul Haque
Summary: This study identified potential inhibitors of SdiA in Klebseilla pneumoniae using computational techniques, with WAY-390139-A showing promising binding affinity. Molecular docking and simulation confirmed the stability and high affinity of WAY-390139-A towards SdiA.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Infectious Diseases
Wael A. H. Hegazy, Ibrahim M. Salem, Hadil Faris Alotaibi, El-Sayed Khafagy, Doaa Ibrahim
Summary: The study finds that the alpha-adrenergic antagonist terazosin has an anti-virulence effect on S. Typhimurium. Terazosin reduces the adhesion, biofilm formation, invasion, and intracellular replication of S. Typhimurium. It also interferes with quorum sensing and bacterial espionage, as well as down-regulates the expression of the type three secretion system (T3SS) encoding genes.
Article
Biochemistry & Molecular Biology
Amber B. Sauder, Melissa M. Kendall
Summary: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 relies on sRNAs, particularly MavR, to coordinate expression of metabolic and virulence factors for host colonization. MavR protects the eutR transcript from degradation, promoting EutR expression, ethanolamine utilization, and ultimately affecting EHEC's colonization ability.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Microbiology
Xiaojie Zhang, Baobao Liu, Xueyan Ding, Peng Bin, Yang Yang, Guoqiang Zhu
Summary: The QS system plays a central role in the regulation of virulence in Salmonella, and blocking this system may be a new strategy for treating Salmonella infection. Studying the application of QQ technology in Salmonella infection can provide insight into the regulation mechanism of bacterial virulence.
Article
Biology
Gita Naseri, Hannah Raasch, Emmanuelle Charpentier, Marc Erhardt
Summary: In this study, a set of arabinose-inducible artificial transcription factors (ATFs) were developed using CRISPR/dCas9 and Arabidopsis-derived DNA-binding proteins to control gene expression in E. coli and Salmonella over a wide range of inducer concentrations. The transcriptional output of the ATFs varied greatly, particularly in Salmonella strains rewired for arabinose catabolism. The developed ATFs were used to engineer a Salmonella biosensor strain and regulate beta-carotene biosynthesis in E. coli, resulting in significantly higher production compared to traditional methods.
COMMUNICATIONS BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Sarika Rana, Sonalika Maurya, Gayatree Mohapatra, Savita Singh, Rohan Babar, Hridya Chandrasekhar, Garima Chamoli, Deepak Rathore, Pallavi Kshetrapal, C. Srikanth
Summary: Non-typhoidal Salmonella infections can lead to chronic infections, where the bacteria utilize complex strategies to reprogram macrophages and create a suitable replicative niche. The upregulation of histone H3-lysine 27 trimethylation-specific demethylase KDM6B plays a crucial role in Salmonella infection, affecting gene targets like PPAR delta and leading to pathogen load reduction in chronic infection models through epigenetic reprogramming.
Article
Microbiology
Abrar K. Thabit, Khalid Eljaaly, Ayat Zawawi, Tarek S. Ibrahim, Ahmed G. Eissa, Samar S. Elbaramawi, Wael A. H. Hegazy, Mahmoud A. Elfaky
Summary: The study found that atenolol can effectively reduce the biofilm formation, invasion, and replication of Salmonella Typhimurium. Atenolol affects the virulence of Salmonella by downregulating related genes and protects mice from infection. In silico molecular docking study showed that atenolol can compete with the target proteins of Salmonella.
Article
Immunology
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Summary: This study demonstrates that the expression levels of STRraA, a protein regulator of STRNase E activity, affect Salmonella enterica serovar Typhimurium pathogenicity by protecting fliDST mRNA from STRNase E cleavage, leading to enhanced flagellar assembly.
MICROBIAL PATHOGENESIS
(2022)
Article
Oncology
Laura Liebscher, Christine Weissenborn, Stefanie Langwisch, Bjoern-Oliver Gohlke, Robert Preissner, Gabriel A. Rabinovich, Nina Christiansen, Holger Christiansen, Ana Claudia Zenclussen, Stefan Fest
Summary: Gal-1 negatively regulates CD8+ T cells in NB, while vaccination with DNA plasmids encoding Gal-1 epitopes enhances immunity and effectively suppresses NB tumor growth.
Article
Food Science & Technology
Zixuan Xu, Zhenyang Hu, Zhilong Yu, Lijun Huang, Fangwei Yang, Yunfei Xie
Summary: Virtual screening targeting LuxS and LsrB proteins of Salmonella was used to identify 12 quorum sensing inhibitors (QSIs) applicable in the food industry. Among them, esculetin showed the lowest MIC and demonstrated significant inhibitory effects on Salmonella's motility, biofilm formation, extracellular polymer, and AI-2 signaling molecule production. Esculetin down-regulated the expression of genes related to quorum sensing and biofilm formation. This study highlights the potential of virtual screening and esculetin as a preservative in the food industry.
Article
Biochemistry & Molecular Biology
Sanne Wolput, Angela Makumi, Laura Wicke, Leonard E. Baecker, William Cenens, Yves Briers, Nicolas A. Wenner, Sian V. Owen, Jay C. D. Hinton, Rob Lavigne, Abram Aertsen
Summary: Many phage genes, called ORFans, have no similarity to other genes in current databases. These ORFan genes can have a significant impact on phage propagation and host interactions, but are often unexplored in experiments. In a previous study, we discovered a novel interaction between phage P22 and its host Salmonella Typhimurium, involving the ORFan gene pid. The pid gene is highly expressed in phage carrier cells that harbor a polarly located P22 episome, which segregates unequally among daughter cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Nikhil K.c, Laxmi Noatia, Swagatika Priyadarsini, M. Pashupathi, Jagan Mohanarao Gali, M. Ayub Ali, S. K. Behera, Bhaskar Sharma, Parimal Roychoudhury, Ajay Kumar, Parthasarathi Behera
Summary: Recoding of the anaerobic regulator gene fnr in Salmonella Typhimurium significantly compromises its growth, motility, biofilm forming ability, and survival within macrophages. Additionally, recoding reduces the colonization ability and fecal shedding of the mutant strain in mice. These findings highlight the attenuated pathogenicity of Salmonella Typhimurium after recoding the global anaerobic regulator gene fnr.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Mustapha Berri, Daniel Hogan, Georges Saade, Sylvie Roche, Philippe Velge, Isabelle Virlogeux-Payant, Francois Meurens
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Florent Kempf, Pierrette Menanteau, Ivan Rychlik, Tereza Kubasova, Jerome Trotereau, Isabelle Virlogeux-Payant, Samantha Schaeffer, Catherine Schouler, Rosanna Drumo, Edouard Guitton, Philippe Velge
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Microbiology
Emilie Barilleau, Megane Vedrine, Michael Koczerka, Julien Burlaud-Gaillard, Florent Kempf, Olivier Grepinet, Isabelle Virlogeux-Payant, Philippe Velge, Agnes Wiedemann
Summary: PagN, a well-conserved protein among different species and subspecies of Salmonella, interacts with heparan sulfate proteoglycan (HSPG) to mediate invasion, but this interaction alone is not sufficient for internalization mechanism. Instead, PagN induces a phosphatidylinositol 3-kinase signaling pathway, leading to a Zipper-like entry mechanism similar to the Salmonella outer membrane protein Rck.
Article
Multidisciplinary Sciences
S. Holbert, E. Barilleau, S. M. Roche, J. Trotereau, S. Georgeault, J. Burlaud-Gaillard, A. Wiedemann, S. Meresse, I Virlogeux-Payant, P. Velge
Summary: Numerous studies have shown that the Salmonella Pathogenicity Island 1-encoded type III secretion system (T3SS1) apparatus and its associated effectors play a key role in the invasion and intracellular fate of Salmonella in host cells. Some cell lines have been identified where Salmonella Typhimurium can enter without using its three known invasion factors. In the murine AML12 hepatocyte cell line, the T3SS1 mutant induced an intracellular fate similar to the wild-type strain, indicating that invasion and survival of Salmonella Typhimurium can be independent of T3SS1.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
S. M. Roche, S. Holbert, Y. Le Vern, C. Rossignol, A. Rossignol, P. Velge, I Virlogeux-Payant
Summary: The study found that liver, spleen, gall bladder and aortic vessels could be sites of infection in chicks, with phagocytic and non-phagocytic cells being invaded in every organ studied. Mutants lacking T3SS-1, Rck and PagN were still able to colonize organs and invade non-phagocytic cells, indicating these known invasion factors are not necessary for entry into these cell types.
Article
Microbiology
Michael Koczerka, Pierre-Emmanuel Douarre, Florent Kempf, Sebastien Holbert, Michel-Yves Mistou, Olivier Grepinet, Isabelle Virlogeux-Payant
Summary: The study revealed the widespread presence of the rck gene in various subspecies of Salmonella, raising questions about its evolution. The results emphasize the important role of rck in virulence and contribute to understanding the role of Rck in the pathogenesis of Salmonella.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Helit Cohen, Claire Hoede, Felix Scharte, Charles Coluzzi, Emiliano Cohen, Inna Shomer, Ludovic Mallet, Sebastien Holbert, Remy Felix Serre, Thomas Schiex, Isabelle Virlogeux-Payant, Guntram A. Grassl, Michael Hensel, Helene Chiapello, Ohad Gal-Mor
Summary: Salmonella enterica includes various serovars causing distinct diseases in humans. In this study, we compared the global gene expression and intracellular phenotypes of S. Typhimurium (STM) and S. Paratyphi A (SPA), representative serovars of non-typhoidal and typhoidal Salmonella, during human epithelial cell infection. We found different expression patterns in key virulence and metabolic pathways, cytosolic motility, and reinvasion ability of SPA after exiting infected cells. These differences may contribute to the invasive and systemic disease developed following SPA infection in humans.
Review
Microbiology
Sandrine Menard, Sonia Lacroix-Lamande, Katrin Ehrhardt, Jin Yan, Guntram A. Grassl, Agnes Wiedemann
Summary: Salmonella enterica serovars are invasive gram-negative bacteria that can cause a wide range of diseases. They gain access to the intestinal lumen and develop mechanisms to cross the intestinal epithelium and survive host defense mechanisms. This review focuses on the molecular dialogue between Salmonella Typhimurium (STm) and the intestinal epithelium, highlighting the mechanisms involved in crossing the intestinal epithelium and surviving host defense mechanisms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jerome Trotereau, Romain Jouan, Delphine Naquin, Maxime Branger, Catherine Schouler, Philippe Velge, Peter Mergaert, Isabelle Virlogeux-Payant
Summary: In this study, a high-density transposon sequencing library of the Salmonella Typhimurium ATCC 14028 strain was constructed and characterized, and essential genes for growth in rich culture medium were identified.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Review
Veterinary Sciences
Martin Beaumont, Fany Blanc, Claire Cherbuy, Giorgia Egidy, Elisabetta Giuffra, Sonia Lacroix-Lamande, Agnes Wiedemann
Summary: Culturing farm animal intestinal organoids has become a valuable tool in research, enabling efficient reduction of live animal use and exploration of physiological and pathological aspects of the intestine.
VETERINARY RESEARCH
(2021)
Article
Immunology
Julien Mambu, Emilie Barilleau, Laetitia Fragnet-Trapp, Yves Le Vern, Michel Olivier, Guillaume Sadrin, Olivier Grepinet, Frederic Taieb, Philippe Velge, Agnes Wiedemann
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)