期刊
MOLECULAR MICROBIOLOGY
卷 81, 期 3, 页码 676-688出版社
WILEY
DOI: 10.1111/j.1365-2958.2011.07722.x
关键词
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资金
- European Molecular Biology Organization (EMBO)
- ERC [260853]
- EU
- NWO-ALW/ERASysBio
- ZonMW
- NWO-ALW
- European Research Council (ERC) [260853] Funding Source: European Research Council (ERC)
Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacillus subtilis. In contrast to B. subtilis, smc is not essential in S. pneumoniae, and Delta smc cells do not show an increased sensitivity to gyrase inhibitors or high temperatures. However, deletion of smc and/or parB results in a mild chromosome segregation defect. Our results show that S. pneumoniae contains a functional chromosome segregation machine that promotes efficient chromosome segregation by recruitment of SMC via ParB. Intriguingly, the data indicate that other, as of yet unknown mechanisms, are at play to ensure proper chromosome segregation in this organism.
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