Article
Biochemistry & Molecular Biology
Srinivasan Ramakrishnan, Rodrigo P. Baptista, Beejan Asady, Guozhong Huang, Roberto Docampo
Summary: In Trypanosoma brucei, down-regulation of Vps41 expression through RNAi leads to significant inhibition of endocytosis, affecting cell growth, while other functions of Vps41 in mammalian and yeast cells remain unaffected. The essentiality of TbVps41 suggests it as a potential drug target.
Article
Immunology
Yan-Zi Wen, Hao-Tian Tang, Xiao-Li Cai, Na Wu, Jia-Zhen Xu, Bi-Xiu Su, Geoff Hide, Zhao-Rong Lun, De-Hua Lai
Summary: In this study, PAG3 was identified as a key nuclear gene involved in the slender to stumpy differentiation pathway of Trypanosoma brucei in the mammalian host. The loss of this gene might explain the inability of T. evansi and some T. equiperdum to differentiate and the adaptation to transmission cycles that bypass the tsetse vector or mechanical contact.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Justin Y. Toh, Agathe Nkouawa, Saul Rojas Sanchez, Huafang Shi, Nikolay G. Kolev, Christian Tschudi
Summary: By targeting 86 transcripts through RNAi, 22 genes that positively or negatively regulate the progression towards infectivity in Trypanosoma brucei have been identified, including the previously unknown nucleic acid binding proteins CSD1 and CSD2. The study suggests a considerable overlap between the genes that regulate stumpy bloodstream form Trypanosoma and those that govern the development of metacyclic form parasites.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Gerardo Priotto, Jose R. Franco, Veerle Lejon, Philippe Buscher, Enock Matovu, Joseph Ndung'u, Sylvain Bieler, Dieudonne Mumba, Nick Van Reet, Paul Verle, Vincent Jamonneau, Pere P. Simarro, Augustin Kadima Ebeja, Dieudonne Sankara, Daniel Argaw Dagne
Summary: Human African trypanosomiasis is a parasitic infection transmitted by the tsetse fly in sub-Saharan Africa. Efforts to eliminate transmission of the disease by 2030 have led to the development of a target product profile for a method to verify the absence of transmission. This method needs to be performed in national or sub-national reference laboratories and have characteristics such as simple specimen collection, no cold-chain requirement, high sensitivity and specificity, high-throughput automated testing, low cost per specimen, and applicability to animals.
BULLETIN OF THE WORLD HEALTH ORGANIZATION
(2023)
Article
Public, Environmental & Occupational Health
Gerardo Priotto, Jose R. Franco, Veerle Lejon, Philippe Buescher, Enock Matovu, Joseph Ndung'u, Sylvain Bieler, Dieudonne Mumba, Nick Van Reet, Paul Verle, Vincent Jamonneau, Pere P. Simarro, Augustin Kadima Ebeja, Dieudonne Sankara, Daniel Argaw Dagne
Summary: Rhodesiense human African trypanosomiasis is a lethal parasitic infection caused by Trypanosoma brucei rhodesiense and transmitted by tsetse flies in eastern and southern Africa. It accounts for approximately 5% of all cases of human African trypanosomiasis. Currently, there is no simple serological test for rhodesiense human African trypanosomiasis and diagnosis relies on microscopic confirmation of trypanosomes in samples of blood or other tissues.
BULLETIN OF THE WORLD HEALTH ORGANIZATION
(2023)
Article
Biochemistry & Molecular Biology
Cecilia Ortiz, Francesca Moraca, Marc Laverriere, Allan Jordan, Niall Hamilton, Marcelo A. Comini
Summary: G6PDH plays a crucial role in cell physiology by catalyzing the synthesis of NADPH(+) and ribose 5-phosphate. The study discovered that 16 alpha-brominated epiandrosterone is the most potent inhibitor of G6PDH in trypanosomatids. Further investigations showed that bromination at position 16 alpha of androstane derivatives yielded more potent T. cruzi G6PDH inhibitors.
Article
Biochemistry & Molecular Biology
Mauro Serricchio, Carolina Hierro-Yap, David Schadeli, Hisham Ben Hamidane, Andrew Hemphill, Johannes Graumann, Alena Zikova, Peter Butikofer
Summary: Cardiolipin plays a crucial role in Trypanosoma brucei bloodstream forms, where its depletion leads to decreased ATP levels and mitochondrial membrane potential, affecting respiration and cellular energy metabolism pathways. Additionally, the loss of cardiolipin also impacts protein stability, particularly those associated with oxygen consumption.
Article
Public, Environmental & Occupational Health
Gerardo Priotto, Jose R. Franco, Veerle Lejon, Philippe Buscher, Enock Matovu, Joseph Ndung'u, Sylvain Bieler, Dieudonne Mumba, Nick Van Reet, Paul Verle, Vincent Jamonneau, Pere P. Simarro, Augustin Kadima Ebeja, Dieudonne Sankara, Daniel Argaw Dagne
Summary: The incidence of human African trypanosomiasis has significantly decreased due to sustained efforts. The World Health Organization has developed a target product profile for assessing T. b. gambiense infection in low-prevalence settings. The profile includes characteristics such as minimal invasiveness, good sensitivity, and long-term stability.
BULLETIN OF THE WORLD HEALTH ORGANIZATION
(2023)
Article
Public, Environmental & Occupational Health
Gerardo Priotto, Jose R. Franco, Veerle Lejon, Philippe Buscher, Enock Matovu, Joseph Ndung'u, Sylvain Bieler, Dieudonne Mumba, Nick Van Reet, Paul Verle, Vincent Jamonneau, Pere P. Simarro, Augustin Kadima Ebeja, Dieudonne Sankara, Daniel Argaw Dagne
Summary: Human African trypanosomiasis is a life-threatening parasitic infection endemic to sub-Saharan Africa, mainly caused by Trypanosoma brucei gambiense. The current diagnostic tests are not accurate, and parasitological confirmation requires specialized techniques. Therefore, a simple and accurate diagnostic tool is needed to identify individuals with the infection and improve treatment. The World Health Organization's Neglected Tropical Diseases Diagnostics Technical Advisory Group has developed a target product profile for such a tool, which should be easy to use, require minimal training, and have a low cost.
BULLETIN OF THE WORLD HEALTH ORGANIZATION
(2023)
Article
Biochemistry & Molecular Biology
Yasuhiro Kurasawa, Kyu Joon Lee, Huiqing Hu, Kieu T. M. Pham, Ziyin Li
Summary: The study demonstrates that TbPLK and TbAUK1 phosphorylate the cytokinesis regulators CIF1 and CIF2 in Trypanosoma brucei. TbPLK interacts with CIF1 in S/G2 phases, maintaining its localization until early mitosis; TbAUK1 maintains CIF1 and CIF2 localization from late mitosis.
Article
Multidisciplinary Sciences
Emma M. Briggs, Federico Rojas, Richard McCulloch, Keith R. Matthews, Thomas D. Otto
Summary: This study models the developmental steps of Trypanosoma brucei using oligopeptide-induced differentiation in vitro, capturing the transcriptomes of parasites through single cell transcriptomics. It details the relative order of biological events during asynchronous development, profiles dynamic gene expression patterns, identifies putative regulators, and provides a paradigm for dissecting differentiation events in parasites.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ainsley Mike Antao, Kamini Kaushal, Soumyadip Das, Vijai Singh, Bharathi Suresh, Kye-Seong Kim, Suresh Ramakrishna
Summary: USP48 interacts with and stabilizes the Aurora B protein, regulating its levels during the cell cycle. Knockout of USP48 leads to accumulation of mitotic defects and cytokinesis failure, delaying cell cycle progression. This study highlights the important role of USP48 in cell cycle regulation and maintenance of genomic stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Parasitology
I Chantal, C. Minet, D. Berthier
Summary: A novel culture protocol was proposed to support long-term in vitro growth of three Savannah and Forest types of T. congolense strains, including IL1180, allowing sustainable growth for 18 days in axenic conditions and efficient growth within 30 days after thawing with a new freezing/thawing system. These results are encouraging for future gene studies or therapeutic drug assays on T. congolense.
VETERINARY PARASITOLOGY
(2021)
Article
Microbiology
Ethan Ashby, Lucinda Paddock, Hannah L. Betts, Jingwen Liao, Geneva Miller, Anya Porter, Lindsey M. Rollosson, Carrie Saada, Eric Tang, Serenity J. Wade, Johanna Hardin, Danae Schulz
Summary: This study reveals changes in DNA-interacting bromodomain protein 3 (Bdf3) occupancy at its binding sites as the parasite Trypanosoma brucei transitions from the bloodstream to the insect stage. Additionally, a new binding site appears near the locus responsible for remodeling of parasite surface proteins during transition to the insect stage. Understanding the mechanisms behind host adaptation is important for understanding the life cycle of the parasite.
Article
Oncology
Yi Sun, Zhenfeng Guan, Qiu Sheng, Wanli Duan, Huacai Zhao, Jiancheng Zhou, Qian Deng, Xinqi Pei
Summary: The study reveals that NMT1 is upregulated in bladder cancer and is associated with poor prognosis. Elevated NMT1 promotes cancer progression and inhibits autophagy. Additionally, NMT1 myristoylates LAMTOR1, leading to increased protein stability and lysosomal localization. The NMT1 inhibitor B13 demonstrates anti-tumor effects against bladder cancer cells.
Article
Biochemistry & Molecular Biology
James W. McCormick, Pia D. Vogel, John G. Wise
Article
Pharmacology & Pharmacy
Courtney A. Follit, Shannon R. Woodruff, Pia D. Vogel, John G. Wise, Nicolay V. Tsarevsky
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2017)
Article
Multidisciplinary Sciences
Amila K. Nanayakkara, Courtney A. Follit, Gang Chen, Noelle S. Williams, Pia D. Vogel, John G. Wise
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
John G. Wise
Article
Biochemistry & Molecular Biology
Oleg A. Volkov, Tarek M. Zaida, Petra Voeller, Holger Lill, John G. Wise, Pia D. Vogel
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2009)
Article
Biophysics
John G. Wise, Pia D. Vogel
BIOPHYSICAL JOURNAL
(2008)
Article
Biophysics
Tassilo Hornung, Oleg A. Volkov, Tarek M. A. Zaida, Sabine Delannoy, John G. Wise, Pia D. Vogel
BIOPHYSICAL JOURNAL
(2008)
Article
Biophysics
John G. Wise, Pia D. Vogel
BIOPHYSICAL JOURNAL
(2009)
Article
Biophysics
Tarek M. Zaida, Tassilo Hornung, Oleg A. Volkov, Andrea D. Hoffman, Susan J. Pandey, John G. Wise, Pia D. Vogel
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2008)
Article
Multidisciplinary Sciences
Amila K. Nanayakkara, Pia D. Vogel, John G. Wise
Article
Chemistry, Medicinal
John G. Wise, Amila K. Nanayakkara, Maha Aljowni, Gang Chen, Maisa C. De Oliveira, Lauren Ammerman, Ketetha Olengue, Alexander R. Lippert, Pia D. Vogel
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Pharmacology & Pharmacy
Courtney A. Follit, Frances K. Brewer, John G. Wise, Pia D. Vogel
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2015)
Article
Pharmacology & Pharmacy
Frances K. Brewer, Courtney A. Follit, Pia D. Vogel, John G. Wise
MOLECULAR PHARMACOLOGY
(2014)
Article
Biochemical Research Methods
M Hossann, ZY Li, YW Shi, U Kreilinger, J Büttner, PD Vogel, JM Yuan, JG Wise, WE Trommer
PROTEIN EXPRESSION AND PURIFICATION
(2006)