Article
Cell Biology
Shugeng Zhang, Shuhang Liang, Dehai Wu, Hongrui Guo, Kun Ma, Lianxin Liu
Summary: The study reveals a direct link between the Hippo and mTORC1 pathways in the control of tumour growth by regulating the activation of mTORC1 through the interaction of long noncoding RNA HPR with Raptor. Knockdown or knockout of HPR in breast cancer and cholangiocarcinoma cells led to a reduction in tumour growth, indicating the importance of this crosstalk in tumorigenesis.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Yui Kotani, Mami Sumiyoshi, Megumi Sasada, Toshio Watanabe, Satoshi Matsuda
Summary: This study reveals that Arf1 plays an important role in the activation of mTORC1 and cell proliferation in mast cells, but has little impact on degranulation and cytokine secretion. This finding suggests the potential of Arf1 as a therapeutic target for mast cell proliferative disorders.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Ning Wang, Ke Zhou, Zhi Liang, Ruiqi Sun, Hong Tang, Zhentao Yang, Wentao Zhao, Yiyang Peng, Penghong Song, Shusen Zheng, Haiyang Xie
Summary: A third-generation mTOR inhibitor, RapaLink-1, demonstrates effectiveness in inhibiting T-cell proliferation and prolonging graft survival time in organ transplantation. It achieves this by reducing p-4EBP1 levels in T cells, increasing Treg cell population, and decreasing Th1 and Th17 cell populations, resulting in improved rejection outcomes.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Jia Yao, Hui Yang, Han Wang, Honglin Shi, Yan Jiao, Ying Zhang, Dexi Chen, Hongbo Shi
Summary: ASPP2 inhibits autophagy and induces apoptosis through the mTORC1-ERS pathway in TNF-alpha-induced hepatocyte injury.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Jiaming Fu, Jie Zhang, Tao Jiang, Xiang Ao, Peng Li, Zhengnan Lian, Chenglong Li, Xibing Zhang, Jie Liu, Minjun Huang, Zhongmin Zhang, Liang Wang
Summary: The study found that mTORC1 promoted HO by regulating TDs chondrogenesis partly through the NF-cB signaling pathway, and rapamycin could be a viable HO therapeutic regimen.
Article
Biotechnology & Applied Microbiology
Yuan-Yuan Jia, Yu-Li Xie, Lu-Lu Yang, Hong-Ling Shi, Yun-Feng Lu, Si-Pu Zhang, Cun-Duo Tang, Lun-Guang Yao, Yun-Chao Kan
Summary: In this study, four hypothetical leucine dehydrogenases were discovered, with PfLeuDH showing a stronger advantage, and the highly selective biosynthesis of L-Tle was successfully achieved. Efficient biosynthesis of L-Tle was carried out by whole-cell catalysis using engineered E. coli cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nutrition & Dietetics
Keita Kanzaki, Masanobu Wada
Summary: This study aimed to investigate the effects of leucine ingestion and muscle contraction on the interaction between Sestrin1/2 and GATOR2 and on mTORC1 activation. The results showed that leucine ingestion led to the dissociation of Sestrin1 from GATOR2, while muscle contraction did not increase the dissociation of Sestrin1/GATOR2. This suggests that Sestrin1 regulates leucine-related mTORC1 activation through its dissociation from GATOR2.
JOURNAL OF NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Ru Wang, Jia Jianyao, Xue Liu, Chen Yaru, Qing Xu, Feng Xue
Summary: This study presents a straightforward method for preparing multi-enzyme nano-devices with high catalytic activity and stability. By fusing molecular binding proteins SpyCatcher and SpyTag with leucine dehydrogenase and glucose dehydrogenase, sc-LeuDH and GDH-st were produced. The cross-linked LeuDH-GDH showed good pH and temperature stability, and further immobilization on ZIF-8 resulted in LeuDH-GDH@ZIF-8 with even better stability and higher yield. This work provides a novel approach for developing multi-enzyme nano-devices with potential applications in complex environments.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Masako Seike, Shun-ichiro Asahara, Hiroyuki Inoue, Michiyo Kudo, Ayumi Kanno, Aisha Yokoi, Hirotaka Suzuki, Maki Kimura-Koyanagi, Yoshiaki Kido, Wataru Ogawa
Summary: In this study, we identified a molecule called TSC2 that inhibits the activity of mTORC1. Further investigation of TSC2's function in pancreatic β cells may help elucidate the mechanism of diabetes and insulin secretion failure.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Zewang Guo, Denghui Chen, Qi Xiong, Miao Liang, Pengfei Li, Zehui Gong, Junzhi Qiu, Liaoyuan Zhang
Summary: In this study, a new LeuDH named PbLeuDH was heterologously over-expressed in Escherichia coli, followed by purification and characterization. PbLeuDH exhibited excellent catalytic efficiency for TMP and was used for high-yield L-Tle production through whole-cell biocatalysis. This research provides a competitive biocatalyst for green biosynthesis of L-Tle.
Article
Microbiology
Ana Caroline de Oliveira Junqueira, Nadielle Tamires Moreira Melo, Nadia Skorupa Parachin, Hugo Costa Paes
Summary: Cytosolic pyruvate is essential for lactic acid production during microbial fermentation. In this study, the MPC1 gene was deleted in a lactate-producing K. phaffii strain, and the impact of this deletion on lactic acid production and cell growth was evaluated. Results showed that unlike other microorganisms, the deletion of Mpc1 had no significant effect on lactic acid production but negatively affected cell growth in K. phaffii strains. Furthermore, the expression of hemoglobin gene indicated functional hypoxia even in aerobic cultivation, suggesting that blocking cell respiration using hypoxia is more suitable for lactic acid production in K. phaffii than deleting Mpc.
Article
Biotechnology & Applied Microbiology
Yufu Zhang, Haibo Xiong, Zhichao Chen, Yunpeng Fu, Qingyang Xu, Ning Chen
Summary: The study revealed that optimizing L-leucine production through chemostat culture can increase yield, improve productivity, and reduce by-products, showing greater potential for industrial production compared to traditional fed-batch approaches.
Article
Biochemistry & Molecular Biology
Xin Li, Changzheng Gao, Wanqing Wei, Wei Song, Weiwei Meng, Jia Liu, Xiulai Chen, Cong Gao, Liang Guo, Liming Liu, Jing Wu
Summary: An efficient method for the asymmetric synthesis of L-ABA was developed using a cost-effective L-Thr. Protein engineering generated an optimal triple mutant BtLDH(M3) with increased activity and conversion rate. Optimized transformation conditions achieved one-pot conversion of L-Thr to 130.2 g/L L-ABA with high yield and enantioselectivity.
Article
Biotechnology & Applied Microbiology
Xiaohu Ding, Wenjun Yang, Xiaobin Du, Ning Chen, Qingyang Xu, Minhua Wei, Chenglin Zhang
Summary: In this study, an efficient L-leucine-producing Escherichia coli strain was developed through rational genetic modifications, achieving the highest production efficiency of L-leucine to date. The strategies presented here will be useful for industrial-scale production of L-leucine and related products.
METABOLIC ENGINEERING
(2023)
Article
Genetics & Heredity
Xiaoqian Cheng, Hantao Wang, Hengling Wei, Lijiao Gu, Pengbo Hao, Huiru Sun, Aimin Wu, Shuaishuai Cheng, Shuxun Yu
Summary: The MADS-box gene family, including AP1, plays an important role in flower development. Through studying the cotton gene GhAP1.7, it was found to potentially act as a positive regulator in plant flowering and be negatively regulated by the LFY gene.
Article
Construction & Building Technology
Ali Ghamari, Amir Johari Naeimi
Summary: Steel plate shear walls have good ductility, high strength, and considerable energy absorption capability. The high-performance steel plate shear wall (HPSPSW) system is expected to be more economical and has desirable stiffness, ductility, and energy absorption when its length to height ratio is greater than 1.5.
PROCEEDINGS OF THE INSTITUTION OF CIVIL ENGINEERS-STRUCTURES AND BUILDINGS
(2023)
Article
Construction & Building Technology
Ali Ghamari, Seyed Majid Amini, Carmen De Crescenzo, Dino Musmarra
Summary: This paper investigates extended end-plate connections equipped with shape memory alloy bolts. A hybrid connection, using shape memory alloy bolts for the outer rows and conventional HSS bolts for the remaining rows, improves the energy absorption capability. Conventional and hybrid connections have better energy absorption capabilities compared to shape memory alloy connections. Increasing pre-stressing over 50% f(y) has no significant effect on the cyclic performance of connections.
PROCEEDINGS OF THE INSTITUTION OF CIVIL ENGINEERS-STRUCTURES AND BUILDINGS
(2023)
Article
Oncology
Sina Azadnajafabad, Sahar Saeedi Moghaddam, Mohammad Keykhaei, Parnian Shobeiri, Negar Rezaei, Erfan Ghasemi, Esmaeil Mohammadi, Naser Ahmadi, Azin Ghamari, Sarvenaz Shahin, Nazila Rezaei, Mahdi Aghili, Ahmad Kaviani, Bagher Larijani, Farshad Farzadfar
Summary: Breast cancer is the most prevalent and deadly neoplasm in females, which poses a heavy burden on health systems. This study aimed to evaluate the quality of care and management of breast cancer patients and its risk factors. The findings provide valuable insights into the epidemiology and burden of breast cancer as well as the quality of care provided to patients with breast cancer.
Article
Oncology
Lev Gorfinkel, Franziska Wachter, Hong-Yuan Luo, Eileen Hansbury, David A. Williams, Archana Agarwal, David H. K. Chui, Alan B. Cantor
Summary: Unstable gamma globin variants can cause transient neonatal hemolytic anemia. We identified a novel variant, termed Hb Wareham, in a newborn with jaundice and anemia. The patient carried the HGB2:c.290T>C, p.Leu97Pro mutation, which is expected to generate an unstable hemoglobin with increased oxygen affinity based on a homologous mutation in the beta globin gene (Hb Debrousse). The patient fully recovered by 9 months of age as expected with the transition from fetal to adult hemoglobin.
PEDIATRIC BLOOD & CANCER
(2023)
Article
Cardiac & Cardiovascular Systems
Sogol Koolaji, Yeganeh Sharifnejad Tehrani, Sina Azadnajafabad, Sahar Saeedi Moghaddam, Sarvenaz Shahin, Azin Ghamari, Naser Ahmadi, Negar Rezaei, Mohammad Keykhaei, Hamed Tavolinejad, Seyyed-Hadi Ghamari, Mohsen Abbasi-Kangevari, Nazila Rezaei, Bagher Larijani, Farshad Farzadfar
Summary: This study aimed to report the national and subnational burden of ischemic heart disease (IHD) and its risk factors in Iran. The results showed a significant decrease in age-standardized death and disability-adjusted life years (DALYs) rates, but only a small reduction in the incidence rate. High blood pressure and elevated LDL-C were the main contributing factors to mortality and DALYs. Primary prevention strategies should be promoted to control the growing risk factors like high fasting plasma glucose (FPG) and high body mass index (BMI).
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2023)
Article
Multidisciplinary Sciences
Narges Ebrahimi, Sarvenaz Shahin, Sogol Koolaji, Ali Ghanbari, Parinaz Mehdipour, Masoud Masinaei, Sahar Saeedi Moghaddam, Negar Rezaei, Azin Ghamari, Mohammad-Reza Malekpour, Nazila Rezaei, Hamidreza Jamshidi, Bagher Larijani, Ardeshir Khosravi, Farshad Farzadfar
Summary: Through various models and data sources, Iran has achieved Sustainable Development Goal 3.2 regarding Under-5 and neonatal mortality rates during 2010-2017. However, there are provincial inequalities that need to be addressed through precise planning for neonatal health care.
Review
Green & Sustainable Science & Technology
Rokiah Othman, Ramadhansyah Putra Jaya, Youventharan Duraisamy, Mohd Arif Sulaiman, Beng Wei Chong, Ali Ghamari
Summary: Foamed concrete is a lightweight construction material with excellent thermal and acoustic insulation properties. However, its production involving cement contributes to a high carbon footprint. This study aims to evaluate the efficiency of industrial waste as a cement replacement in foamed concrete production and found that efficient waste can promote the use of waste materials, reduce CO2 emissions, conserve energy and resources, and improve the properties of foamed concrete.
Article
Engineering, Civil
Ali Ghamari, Radhwan Alzeebaree, Chanachai Thongchom
Summary: This study investigates the use of X-stiffeners to enhance the stiffness characteristics of metallic shear dampers. Numerical and parametric evaluations demonstrate that the ultimate strength and elastic stiffness of shear dampers are significantly improved by adding X-stiffeners.
Article
Construction & Building Technology
Leila Hosseinzadeh, Ali Ghamari
Summary: Studies have shown that corrugated steel shear walls (CSSWs) have a higher energy absorption and shear buckling capacity compared to flat plates. This article examines the effect of changing the angle of the trapezoidal panel on the behavior of CSSWs. Experimental results indicate that increasing the corrugation angle leads to increased stress concentration in the corner of subpanels, as well as decreased stiffness and energy dissipation. Numerical models also show that seismic factors are influenced by the corrugation angle, but there is no clear relationship between varying parameters and changing angles. Furthermore, increasing frame stiffness does not necessarily improve all seismic parameters in CSSWs.
STEEL CONSTRUCTION-DESIGN AND RESEARCH
(2023)
Article
Public, Environmental & Occupational Health
Ali Akbar Ghamari, Keivan Amini, Amin Daei Sorkhabi, Aila Sarkesh, Seyed Hadi Saghaleini, Roghayeh Asghari, Mansour Rezayi, Ata Mahmoodpoor
Summary: This study aimed to investigate the predictive factors of weaning outcome before and during spontaneous breathing trial (SBT) in mechanically ventilated patients. The findings revealed that increased central venous pressure (CVP) was positively correlated with extubation/weaning failure.
HEALTH SCIENCE REPORTS
(2023)
Article
Engineering, Civil
Alireza Abbaszadeh, Ali Ghamari, Vahid Broujerdian
Summary: This study presents the numerical simulation of the seismic behavior of a recently developed four-layer steel shear wall composed of corrugated and flat sheets. The results showed that the corrugated sheets surrounded by flat sheets provide greater seismic parameters such as ductility and over-strength compared to double corrugated walls.
KSCE JOURNAL OF CIVIL ENGINEERING
(2023)
Article
Construction & Building Technology
Chung Nguyen Van, Ali Ghamari
Summary: This paper presents an innovative damper to overcome the low energy capacity of concentrically braced frames (CBFs) and provides the required equations for designing the system. Experimental results show that the damper has stable hysteresis loops up to a rotation of 12%, which is 50% greater than the AISC limitation. Numerical studies indicate that increasing the angle of the main plates improves the structural parameters, such as ultimate strength, stiffness, energy absorption, and overstrength.
STRUCTURAL DESIGN OF TALL AND SPECIAL BUILDINGS
(2023)
Article
Construction & Building Technology
Seong-Hoon Jeong, Ali Ghamari, Akram Jawdhari
Summary: An innovative metallic damper with a flexural mechanism is proposed to improve the behavior of concentrically braced frame systems. Experimental and numerical evaluations show that the damper provides stable hysteresis curves and high energy absorption capacity, with the IFP damper outperforming the RFP damper.
STRUCTURAL DESIGN OF TALL AND SPECIAL BUILDINGS
(2023)
Article
Materials Science, Multidisciplinary
Ali Ghamari, Seong-Hoon Jeong, Chanachai Thongchom, Ramadhansyah Putra Jaya
Summary: This study investigates the strengthening of pipe dampers to improve their strength and stiffness, aiming to overcome their shortcomings. The results show that reducing the length to the height ratio and increasing the flange-to-web strength ratio can enhance the performance of the damper.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2023)
Article
Construction & Building Technology
Akbar Rasoulnia, Vahid Broujerdian, Ali Ghamari, Abbas Ghadami
Summary: The present study aims to characterize the behavior and flexural strength of steel plate I-girders under elevated temperatures. A new design procedure and flexural design curves were proposed to predict the flexural behavior and estimate the ultimate flexural strength of the I-girders. The procedure quantifies and formulates the effects of elevated temperatures to find an ambient-temperature equivalent of the I-girder. Numerical simulations and calibration of the design procedure achieved good conformity between the numerical and parametric results.
STRUCTURAL DESIGN OF TALL AND SPECIAL BUILDINGS
(2023)