期刊
MOLECULAR MEDICINE REPORTS
卷 8, 期 4, 页码 967-972出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2013.1640
关键词
alantolactone; colorectal cancer; nature compounds; apoptosis
资金
- Fundamental Research Funds for the Central Universities [11QNJJ021]
- National Natural Science Foundation of China [30873409, 30670220, 31070318]
- Cultivation Fund of the Scientific and Technical Innovation Project of Northeast Normal University [NENU-STB07008]
- Administration of Traditional Chinese Medicine of Jilin Province [2010pt068, 2011-zd17]
- Research Foundation of Jilin Provincial Science and Technology Development [20100911, 200805131, 201201065]
Alantolactone, a methanol extract of Inula helenium, possesses anticancer properties in a number of cancer cell lines. However, its anticancer effect on human colorectal cancer cells and the underlying mechanisms remain to be elucidated. In the present study, the effects of alantolactone on cell viability and apoptosis in RKO human colon cancer cells were investigated. Alantolactone treatment of RKO cells was found to result in dose-dependent inhibition of cell viability and induction of apoptosis, accompanied with the accumulation of reactive oxygen species (ROS) and the disruption of mitochondrial membrane potential. In addition, these effects were blocked with N-acetylcysteine, a specific ROS inhibitor. Western blotting indicated that exposure of RKO cells to alantolactone is associated with the downregulation of Bcl-2, induction of Bax and activation of caspase-3 and -9. These results indicated that a ROS-mediated mitochondria-dependent pathway is involved in alantolactone-induced apoptosis. From these observations, it was hypothesized that alantolactone may be used for the treatment of human colon cancer.
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