Article
Cell Biology
Aya Al Katat, Juan Zhao, Angelino Calderone, Lucie Parent
Summary: Intracellular Ca2+ overload caused by chronic hemodynamic stimuli can stimulate Ca2+-dependent signaling and promote cardiomyocyte hypertrophy. The study found that sympathetic-mediated hypertrophy of neonatal rat ventricular cardiomyocytes (NRVMs) led to an increase in calcium influx due to upregulation of Ca(V)1.2 channel subunits. The upregulation of Ca-V alpha 2 delta 1 protein levels was found to be independent of the concomitant hypertrophic response, while the upregulation of Ca-V beta 3 protein levels may be directly dependent on the hypertrophic response of NRVMs.
Article
Biochemistry & Molecular Biology
Maria J. Baier, Jannis Noack, Mark Tilmann Seitz, Lars S. Maier, Stefan Neef
Summary: This study demonstrates that phosphorylation of ryanodine receptor type 2 at Ser-2814 by CaMKII is a critical mechanism for the development of sarcoplasmic reticulum Ca2+ leak under beta 1-adrenergic stress. Cardiomyocytes with a phosphoresistant mutation at Ser-2814 are protected from isoproterenol-induced SR Ca2+ leak and show improved postrest potentiation of systolic Ca2+ release under adrenergic stress compared to wild-type cells.
Article
Pharmacology & Pharmacy
Keisuke Obara, Kento Yoshioka, Montserrat De Dios Regadera, Yusuke Matsuyama, Ayano Yashiro, Mayumi Miyokawa, Rumi Iura, Yoshio Tanaka
Summary: This study examined the involvement of alpha 1L-adrenoceptor in phenylephrine-induced contractions in guinea pig thoracic aorta. It was found that alpha 1L-AR competitively inhibited the contractions and the signaling pathway involved Gq protein activation and calcium influx.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2023)
Editorial Material
Physiology
Beatrice Bretherton, Susan A. Deuchars, Jim Deuchars
EXPERIMENTAL PHYSIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Thomas M. D. Sheard, Miriam E. Hurley, John Colyer, Ed White, Ruth Norman, Eleftheria Pervolaraki, Kaarjel K. Narayanasamy, Yufeng Hou, Hannah M. Kirton, Zhaokang Yang, Liam Hunter, Jung-uk Shim, Alexander H. Clowsley, Andrew J. Smith, David Baddeley, Christian Soeller, Michael A. Colman, Izzy Jayasinghe
Article
Biochemistry & Molecular Biology
Ewan D. Fowler, David Hauton, John Boyle, Stuart Egginton, Derek S. Steele, Ed White
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biology
Gareth W. Fearnley, Izma Abdul-Zani, Antony M. Latham, Monica C. Hollstein, John E. Ladbury, Stephen B. Wheatcroft, Adam F. Odell, Sreenivasan Ponnambalam
Article
Cell & Tissue Engineering
Fatema Mousawi, Hongsen Peng, Jing Li, Ponnambalam Sreenivasan, Sebastian Roger, Hucheng Zhao, Xuebin Yang, Lin-Hua Jiang
Article
Cell Biology
Gareth W. Fearnley, Antony M. Latham, Monica Hollstein, Adam F. Odell, Sreenivasan Ponnambalam
CELLULAR SIGNALLING
(2020)
Article
Hematology
Rhodri J. King, Katharina Schuett, Christian Tiede, Vera Jankowski, Vicky John, Abhi Trehan, Katie Simmons, Sreenivasan Ponnambalam, Robert F. Storey, Colin W. G. Fishwick, Michael J. McPherson, Darren C. Tomlinson, Ramzi A. Ajjan
Summary: The interaction between C3 and fibrinogen plays a crucial role in thrombosis risk, and intervention to disrupt this interaction can reduce the risk of thrombosis. The study identified binding sites between C3 and fibrinogen, and manipulating this interaction can impact the lysis of fibrin clots.
Editorial Material
Physiology
Di Lang, Sarah C. Calaghan, Julia Gorelik, Alexey V. Glukhov
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Biochemical Research Methods
Miriam E. Hurley, Thomas M. D. Sheard, Ruth Norman, Hannah M. Kirton, Shihab S. Shah, Eleftheria Pervolaraki, Zhaokang Yang, Nikita Gamper, Ed White, Derek Steele, Izzy Jayasinghe
Summary: The study introduces a correlative imaging protocol to visualize the direct relationship between the ubiquitous intracellular second messenger, calcium (Ca2+), and nanoscale patterns of the ryanodine receptor (RyR) Ca2+ channels. By combining total internal reflection fluorescence (TIRF) imaging and DNA-PAINT imaging, the data can be used to visually identify the ensembles of Ca2+ channels that are locally activated during the genesis of cytoplasmic Ca2+ signals.
Article
Cell Biology
Yuan-Ming Tsai, Frederick Jones, Pierce Mullen, Karen E. Porter, Derek Steele, Chris Peers, Nikita Gamper
Review
Biochemistry & Molecular Biology
Faheem Shaik, Gary A. Cuthbert, Shervanthi Homer-Vanniasinkam, Stephen P. Muench, Sreenivasan Ponnambalam, Michael A. Harrison
Article
Cell Biology
William R. Critchley, Gina A. Smith, Ian C. Zachary, Michael A. Harrison, Sreenivasan Ponnambalam
Summary: VEGFR2, encoded by KDR, plays a crucial role in endothelial function and angiogenesis. UBE2D1 and UBE2D2 are identified as key regulators of VEGFR2 ubiquitination and proteolysis, affecting VEGF-A signaling and endothelial tubulogenesis.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Miriam E. Hurley, Ed White, Thomas M. D. Sheard, Derek Steele, Izzy Jayasinghe
Summary: Rapid release of calcium from internal stores via ryanodine receptors (RyRs) is one of the fastest types of cytoplasmic second messenger signalling in excitable cells. In the heart, calcium sparks determine myocardial contraction through the summation of elementary events of calcium release. Correlative super-resolution microscopy revealed a close relationship between calcium sparks and the positions of RyR2 clusters, with fragmented clusters observed in failing ventricles.
Review
Cell Biology
Gary A. Cuthbert, Faheem Shaik, Michael A. Harrison, Sreenivasan Ponnambalam, Shervanthi Homer-Vanniasinkam
Article
Chemistry, Multidisciplinary
Jessica L. Haigh, Daniel J. Williamson, Emma Poole, Yuan Guo, Dejian Zhou, Michael E. Webb, Susan A. Deuchars, Jim Deuchars, W. Bruce Turnbull
CHEMICAL COMMUNICATIONS
(2020)