Article
Cell Biology
Rasha S. S. Teleb, Amal Abdul-Hafez, Amira Othman, Ahmed El-Abd Ahmed, Abdelrahman A. A. Elsaid, Hattan Arif, Ahmed A. A. Zarea, Mohammed Abdulmageed, Hend Mohamed, Sherif Abdelfattah Ibrahim, Ranga P. P. Thiruvenkataramani, Tarek Mohamed, Masamitsu Kanada, Burra V. V. Madhukar, Myrna Gonzalez Arellano, Mohammed M. M. Sayed, Heba M. M. Qubaisy, Said A. A. Omar
Summary: The objective of this study was to examine the effect of cord blood plasma (CBP)-derived exosomes (CBP Exos) and placental MSCs-derived exosomes (MSCs Exos) on the expansion of umbilical cord blood hematopoietic stem cells (UCB HSCs) and to maintain their primitive characteristics. The results showed that CBP- and placental-derived exosomes significantly enhanced the ex vivo expansion of UCB HSCs while preserving their primitive features.
Article
Cell & Tissue Engineering
Liem Thanh Nguyen, Nghia Trung Tran, Uyen Thi Trang Than, Minh Quang Nguyen, Anh Minh Tran, Phuong Thi Xuan Do, Thao Thi Chu, Tu Dac Nguyen, Anh Viet Bui, Tien Anh Ngo, Van Thanh Hoang, Nhung Thi My Hoang
Summary: This study evaluated the efficiency of UCB-MSC isolation and expansion using different commercially available media kits and found that StemMACS(TM) MSC Expansion Media is more appropriate. Autologous serum coating and plasma supplement enhanced cell proliferation. UCB-MSCs exhibited functions in promoting angiogenesis and inhibiting cancer cell growth.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell & Tissue Engineering
Wen-zhao Liu, Zhan-jun Ma, Jie-ru Li, Xue-wen Kang
Summary: SCI often leads to serious motor and sensory dysfunction of the limbs below the injured segment, causing physical, psychological harm and economic burden. MSC therapy for SCI has shown promise but faces challenges like low survival rate and immune rejection, while exosomes derived from MSCs have great potential in the treatment of SCI.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Giovanni Schepici, Serena Silvestro, Emanuela Mazzon
Summary: Spinal cord injury (SCI) is a devastating condition that causes permanent motor and sensory deficits. Researchers have been studying potential therapeutic strategies to mitigate the neuro-inflammatory response following injury. Recent studies have shown that exosomes-derived MSCs (MSC-EXOs) have promising effects as an innovative therapy for SCI patients.
Article
Chemistry, Multidisciplinary
Yong Xie, Yi Sun, Yudong Liu, Jinyun Zhao, Quanbo Liu, Jiaqi Xu, Yiming Qin, Rundong He, Feifei Yuan, Tianding Wu, Chunyue Duan, Liyuan Jiang, Hongbin Lu, Jianzhong Hu
Summary: This study explores a specific targeted treatment approach for spinal cord injury by identifying stable subpopulations of exosomes that can improve blood-spinal cord barrier integrity and promote neural function recovery.
Review
Biochemistry & Molecular Biology
Jinsu Hwang, Sujeong Jang, Choonghyo Kim, Sungjoon Lee, Han-Seong Jeong
Summary: Neurological disorders pose a global health problem, and current pharmacological treatments have side effects. Mesenchymal stem cells (MSCs) have anti-inflammatory, anti-apoptotic, and immunomodulatory properties, and their secreted paracrine factors play a therapeutic role. Exosomes and MSC-derived miRNAs also regulate genes and alleviate neuropathological changes in neurological diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Juan Zhang, Xiaoqian Ma, Lu Cao, Xing He, Sang Li, Min Yang, Cejun Yang, Pengfei Rong, Shounan Yi, Kedar Ghimire, Xiangfeng Kong, Wei Wang
Summary: Hucb-Tregs treated with MSC-CM or MSC-derived exosomes were more suppressive and resistant to inflammatory stimulation. MSC-derived exosomes could activate autophagy and JAK3-Stat5 signaling pathway to promote Treg survival and regulate FOXP3 expression. MSC-derived exosomes could be an effective strategy to improve the Tregs-based cell therapy landscape.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Parisa Fayyazpour, Effat Alizadeh, Vahid Hosseini, Ashkan Kalantary-Charvadeh, Mitra Niafar, Vahideh Sadra, Zahra Norouzi, Aysan Saebnazar, Amir Mehdizadeh, Masoud Darabi
Summary: In type 2 diabetes, dyslipidemia and increased serum free fatty acids (FFAs) negatively affect insulin secretion and worsen the disease. This study found that FFAs from diabetic serum have a detrimental effect on the stemness characteristics of adipose-derived mesenchymal stem cells, potentially impairing their regenerative capabilities.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Neurosciences
Hosna Cheshmi, Hossein Mohammadi, Mitra Akbari, Davood Nasiry, Rafat Rezapour-Nasrabad, Mahdi Bagheri, Beheshteh Abouhamzeh, Mahnaz Poorhassan, Mehri Mirhoseini, Hossein Mokhtari, Esmaeil Akbari, Amir Raoofi
Summary: Current treatments for spinal cord injury (SCI) are ineffective in improving sensorimotor function. This study investigated the combined effects of human placenta mesenchymal stem cells (hPMSCs)-derived exosomes and hyperbaric oxygen (HBO) in rat models of SCI. The findings showed that the co-administration of hPMSCs-derived exosomes and HBO significantly improved stereological parameters, biochemical factors, gene expression, and behavioral functions, while reducing oxidative stress, apoptosis, gliosis, and inflammation. Therefore, the combination therapy of hPMSCs-derived exosomes and HBO has synergistic neuroprotective effects in SCI animals.
NEUROTOXICITY RESEARCH
(2023)
Article
Oncology
Guoyi Dong, Xiaojing Xu, Yue Li, Wenjie Ouyang, Weihua Zhao, Ying Gu, Jie Li, Tianbin Liu, Xinru Zeng, Huilin Zou, Shuguang Wang, Yue Chen, Sixi Liu, Hai-Xi Sun, Chao Liu
Summary: In this study, single-cell RNA-seq was used to analyze hematopoietic stem cells (HSCs) from cord blood and mobilized peripheral blood. The source and culture time of HSCs were found to affect their characteristics, and stemness-related genes were identified. The study revealed that maintaining stemness requires dynamic protein translation and processing, and culture time also affects HSCs' homing and engraftment ability. Furthermore, the study identified a small molecule that can expand HSCs while maintaining their stemness.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Jia Chen, Yuhan Chen, Xue Du, Guojun Liu, Xiaowei Fei, Jian Ru Peng, Xing Zhang, Fengjun Xiao, Xue Wang, Xiao Yang, Zhichun Feng
Summary: CB-MNCs and UC-MSCs show efficacy in alleviating BPD, with CB-MNCs having more pronounced effects in restoring lung function. Through analysis of lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA networks, it was found that therapeutic effects are related to regulation of GTPase activity, ERK signaling cascade, chromosome regulation, and cell cycle control.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ewelina Tomecka, Wioletta Lech, Marzena Zychowicz, Anna Sarnowska, Magdalena Murzyn, Tomasz Oldak, Krystyna Domanska-Janik, Leonora Buzanska, Natalia Rozwadowska
Summary: The study found that 5% oxygen concentration had beneficial effects on the proliferation, clonogenicity, and senescence of hWJ-MSCs, while not influencing cell morphology, immunophenotype, or neuroprotective effect. The potential for differentiation into adipocytes, osteocytes, and chondrocytes was comparable under both oxygen conditions, with enhanced neuronal differentiation seen under atmospheric oxygen conditions. Mitochondrial respiration was more important for the cells regardless of oxygen conditions, suggesting that hWJ-MSCs can be effectively cultured under both oxygen conditions, but 5% oxygen level may provide a more balanced environment.
Article
Biochemistry & Molecular Biology
Ilya Shulman, Tatyana Ageeva, Alexander Kostennikov, Sergei Ogurcov, Leysan Tazetdinova, Ilyas Kabdesh, Alexander Rogozhin, Ilnur Ganiev, Albert Rizvanov, Yana Mukhamedshina
Summary: Spinal cord injury (SCI) is a current medical and social problem that causes severe disability. The use of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) is a strategy to promote the recovery of spinal cord structure and function after trauma. This study evaluated the therapeutic potential of repeated intrathecal injection of autologous MSC-derived EVs in a pig model of subacute contused SCI. The results showed partial restoration of locomotor activity through remyelination of axons and timely reperfusion of nervous tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Jiasheng Wang, Leland Metheny
Summary: Cord blood is an important source for allogeneic hematopoietic cell transplantation and is also being developed for cell therapy products. The unique cellular composition of cord blood, including higher percentages of hematopoietic stem cells and progenitor cells, offers advantages in immune tolerance and off-the-shelf cell therapy development. In addition, cord blood-derived mesenchymal stem cells have potential in regenerative medicine and inflammatory/autoimmune conditions. This review focuses on recent clinical development of cord blood-derived therapies in oncology.
FRONTIERS IN ONCOLOGY
(2023)
Article
Endocrinology & Metabolism
Q. Hua, L. Sun, H. R. Li, H. J. Li, B. D. Ma, Y. L. Wang
Summary: The study demonstrates that human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) significantly inhibit the proliferation of human ovarian cancer SKOV3 cells in vitro, possibly by blocking the cell cycle and inducing cell death through apoptosis and autophagy. This indicates the potential of hUCB-MSCs as an effective anticancer adjuvant in the treatment of ovarian cancer.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2021)