期刊
MOLECULAR MEDICINE REPORTS
卷 4, 期 1, 页码 105-111出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2010.382
关键词
cardiovascular heat shock protein; molecular architecture; small heat shock proteins; subunit exchange
资金
- Sichuan University of the Education ministry of China
Cardiovascular heat shock protein (cvHsp) is an abundant and selectively expressed component in cardiac tissue with putative molecular functionality. The most prominent feature of cvHsp is the characteristic alpha-crystallin domain, which makes it a member of the small heat shock protein (sHsp) family. In the present study, we cloned and expressed the cvHsp gene, purified cvHsp to homogeneity, and characterized its structural and molecular properties. The cvHsp mainly consisted of beta-sheets and randomly coiled secondary structural elements, and in addition possessed variable tertiary structures and several solvent-exposed hydrophobic patches on its molecular surface. The purified cvHsp existed as a mixture of dimers and oligomers containing 12 monomers, and exhibited subunit exchange capacity when labeled with AIAS and LYI. In total, the cvHsp was characterized as having an oligomeric molecular architecture, and displayed various properties common to the sHsp family. The cvHsp gene may have physiologically important implications in the cardiac stress response.
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