期刊
MOLECULAR MEDICINE
卷 17, 期 11-12, 页码 1188-1195出版社
SPRINGER
DOI: 10.2119/molmed.2011.00104
关键词
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资金
- Compagnia di San Paolo [4824 SD/CV, 2007.2880]
- Associazione Italiana per la Ricerca sul Cancro (AIRC) [IG-10698]
- Associazione Davide Ciavattini Onlus
- Fondazione Maria Piaggio Casarsa
- King Abdullah University of Science and Technology (KAUST) [KUK-I1-012-43]
- AIRC progetto [RG6432]
- Carical, Fondazione A. Scorza, Provincia di Cosenza
- National Cancer Institute [RO1 CA81554]
- National Center for Research Resources [RR018535]
- Karches Foundation
- Prince Family Foundation
- Marks Foundation
- Jerome Levy Foundation
- Leon Levy Foundation
- Joseph Eletto Leukemia Research Fund
- Fondazione Internazionale in Medicina Sperimentale (FIRMS)
B-cell chronic lymphocytic leukemia (CLL) patients display leukemic clones bearing either germline or somatically mutated immunoglobulin heavy variable (IGHV) genes. Most information on CLL immunoglobulins (Igs), such as the definition of stereotyped B-cell receptors (BCRs), was derived from germline unmutated Igs. In particular, detailed studies on the distribution and nature of mutations in paired heavy- and light-chain domains of CLL clones bearing mutated Igs are lacking. To address the somatic hypermutation dynamics of CLL Igs, we analyzed the mutation pattern of paired IGHV-diversity-joining (IGHV-D-J) and immuno globulin kappa/lambda variable-joining (IGK/LV-J) rearrangements of 193 leukemic clones that displayed >= 2% mutations in at least one of the two immunoglobulin variable (IGV) genes (IGHV and/or IGK/LV). The relationship between the mutation frequency in IGHV and IGK/LV complementarity determining regions (CDRs) and framework regions (FRs) was evaluated by correlation analysis. Replacement (R) mutation frequency within IGK/LV chain CDRs correlated significantly with mutation frequency of paired IGHV CDRs in lambda but not kappa isotype CLL clones. CDRs of IGKV-J rearrangements displayed a lower percentage of R mutations than IGHVs. The frequency/pattern of mutations in kappa CLL Igs differed also from that in K-expressing normal B cells described in the literature. Instead, the mutation frequency within the FRs of IGHV and either IGKV or IGLV was correlated. Notably, the amount of diversity introduced by replaced amino acids was comparable between IGHVs and IGKVs. The data indicate a different mutation pattern between K and X isotype CLL clones and suggest an antigenic selection that, in K samples, operates against CDR variation. (C) 2011 The Feinstein Institute for Medical Research, www.feinsteininstitute.org Online address: http://www.molmed.org doi: 10.2119/molmed.2011.00104
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