Article
Physics, Multidisciplinary
David B. Brueckner, Matthew Schmitt, Alexandra Fink, Georg Ladurner, Johannes Flommersfeld, Nicolas Arlt, Edouard Hannezo, Joachim O. Raedler, Chase P. Broedersz
Summary: This research develops a model for protrusion and polarity dynamics in confined cell migration using data-driven inference and a mechanistic bottom-up approach. The study reveals that cellular dynamics adapt to confining geometries through a switch in polarity dynamics, driving the migration of the cell through constrictions.
Article
Multidisciplinary Sciences
Nikola Lukic, Trishna Saha, Stefanie Lapetina, Michal Gendler, Gilad Lehmann, Anthony J. Koleske, Hava Gil-Henn
Summary: Cell migration is crucial for tissue construction and regeneration, playing key roles in processes such as embryonic development, immune responses, and wound healing. Disruptions in cell motility can lead to pathological disorders, including chronic inflammation and cancer metastasis. Actin polymerization-mediated cell-edge protrusions initiate various cellular processes and can be assessed through a method for quantitative analysis of cell-edge protrusion dynamics.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Multidisciplinary Sciences
Florian Fassler, Manjunath G. Javoor, Julia Datler, Hermann Doring, Florian W. Hofer, Georgi Dimchev, Victor-Valentin Hodirnau, Jan Faix, Klemens Rottner, Florian K. M. Schur
Summary: Regulation of the Arp2/3 complex plays a crucial role in the nucleation of branched actin networks. Incorporation of subunit isoforms, such as ArpC5 and ArpC5L, fine-tunes nucleation activity and branch junction stability, affecting cell migration. Furthermore, ArpC5 isoforms impact the positioning of Ena/VASP proteins, which mediate isoform-specific effects on actin assembly and contribute to a signaling pathway enhancing cell migration.
Article
Microbiology
T. Essock-Burns, B. D. Bennett, D. Arencibia, S. Moriano-Gutierrez, M. Medeiros, M. J. McFall-Ngai, E. G. Ruby
Summary: This study demonstrates that quorum-sensing regulation by the Vibrio fischeri population induces a tissue phenotype that promotes the retention of this extracellular symbiont within the light organ of its host. Actin polymerization is identified as the primary mechanism underlying constriction, and host responses to the presence of symbionts change as a function of tissue maturation.
Article
Biochemistry & Molecular Biology
Abris Adam Bendes, Petri Kursula, Inari Kursula
Summary: Apicomplexan parasites rely on the glideosome for motility and host cell invasion; actin filament capping proteins (CPs) play a crucial role in regulating actin dynamics; this study presents the crystal structure of CP from the malaria parasite and reveals differences compared to canonical heterodimers.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemical Research Methods
Ching-fen Jiang, Yu-man Sun
Summary: In this study, a method for monitoring the density changes of stem cell cytoskeleton without fluorescence staining using phase-contrast microscopy was proposed. The reliability of the model in quantifying texture density and its capability to reflect the spatiotemporal regulation of the cytoskeletal structure were validated. The method was applied to a video of mesenchymal stem cell growth dynamics, demonstrating its effectiveness.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biophysics
Maryna Kapustina, Donna Li, James Zhu, Brittany Wall, Violetta Weinreb, Richard E. Cheney
Summary: Each cell possesses a substantial amount of cell surface excess (CSE) that can be deployed to cover cell extensions and allow rapid changes in morphology. Microtubules (MTs) play a significant role in regulating the storage and release of CSE, impacting cell dynamics and cell shape changes. This finding has implications for understanding cell biomechanics and cell migration.
BIOPHYSICAL JOURNAL
(2023)
Article
Cell Biology
Ioannis Kasioulis, Alwyn Dady, John James, Alan Prescott, Pamela A. Halley, Kate G. Storey
Summary: This study uncovers a new form of cell-cell contact within the developing neuroepithelium, which plays important roles in cell and tissue morphology and cell signalling.
JOURNAL OF CELL SCIENCE
(2022)
Review
Microbiology
Nora Kostow, Matthew D. Welch
Summary: Manipulation of host cell plasma membrane by intracellular bacterial pathogens is crucial for their infection. Bacteria employ various strategies to target and influence the composition and shape of the plasma membrane, taking advantage of pathways such as signal transduction, endocytosis, and exocytosis. Further investigation into the contributions of bacterial and host factors to plasma membrane manipulation will provide valuable insights into pathogenesis and fundamental principles of plasma membrane biology.
CURRENT OPINION IN MICROBIOLOGY
(2023)
Article
Cell Biology
MengWen Huang, Ling Lu, ChangDong Lin, YaJuan Zheng, XingChao Pan, ShiHui Wang, ShiYang Chen, YouHua Zhang, ChunYe Liu, GaoXiang Ge, Yi Arial Zeng, JianFeng Chen
Summary: Dynamic regulation of integrin activation and inactivation is crucial for cell adhesion and migration. LRP12 is identified as a transmembrane inhibitor for a4 integrin activation, maintaining the balance of nascent adhesion turnover and controlling cell migration. Knockdown of LRP12 leads to increased nascent adhesions and enhanced cell migration.
Article
Cell Biology
Hendrik Schuermann, Fatemeh Abbasi, Antonella Russo, Arne D. Hofemeier, Matthias Brandt, Johannes Roth, Thomas Vogl, Timo Betz
Summary: Podosomes are protrusive actin structures that respond to mechanical stimuli and are commonly found in myeloid cells, playing a role in vascular extravasation. This study investigated the force generation of podosome-bearing ER-Hoxb8 monocytes at the cellular level. The findings suggest that the collective contraction of the actomyosin network leads to a buckling mechanism, causing substrate indentation on the cell scale. This additional force generation complements the protrusion forces of individual podosomes.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ernest Iu, Alexander Bogatch, Sergey Plotnikov
Summary: Cell spreading is a dynamic process where a cell attaches to a substrate and forms thin lamellipodia, crucial for cell morphology and mechanical forces. Cell spreading assays are used to assess lamellipodial dynamics, but a detailed experimental workflow and data analysis tools are currently lacking.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Ruping Wang, Eylon Arbel, Dale D. Tang
Summary: This study demonstrates that smooth muscle myosin localizes at the leading edge of cells and orchestrates the recruitment of actin-regulatory proteins, promoting cell migration.
Article
Multidisciplinary Sciences
Yoshihiro Ueda, Koichiro Higasa, Yuji Kamioka, Naoyuki Kondo, Shunsuke Horitani, Yoshiki Ikeda, Wolfgang Bergmeier, Yoshinori Fukui, Tatsuo Kinashi
Summary: Lymphocyte trafficking requires precise regulation of chemokine-mediated cell migration, which depends on cytoskeletal dynamics and polarity. This study reveals that the integrin activator Rap1 plays critical roles in lymphocyte polarization and chemotaxis independent of adhesion. Rap1 activation involves F-actin scaffolds, stimulates RhoA activation and actomyosin contractility at the rear, and requires the downstream effector talin1 for actomyosin contractility in the uropod. These findings provide insights into the regulatory mechanisms of lymphocyte trafficking.
Article
Cell Biology
Mengdie Wang, Choua Xiong, Arthur M. Mercurio
Summary: This study reveals an unexpected intrinsic function of PD-L1 in promoting persistent cell migration by facilitating retraction through interaction with the beta 4 integrin.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Mihai L. Azoitei, Jungsik Noh, Daniel J. Marston, Philippe Roudot, Christopher B. Marshall, Timothy A. Daugird, Sidney L. Lisanza, Maria-Jose Sandi, Mitsu Ikura, John Sondek, Robert Rottapel, Klaus M. Hahn, Gaudenz Danuser
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biochemical Research Methods
Meghan K. Driscoll, Erik S. Welf, Andrew R. Jamieson, Kevin M. Dean, Tadamoto Isogai, Reto Fiolka, Gaudenz Danuser
Review
Biochemical Research Methods
Sandeep Manandhar, Patrick Bouthemy, Erik Welf, Gaudenz Danuser, Philippe Roudot, Charles Kervrann
Article
Multidisciplinary Sciences
Jin Suk Park, Christoph J. Burckhardt, Rossana Lazcano, Luisa M. Solis, Tadamoto Isogai, Linqing Li, Christopher S. Chen, Boning Gao, John D. Minna, Robert Bachoo, Ralph J. DeBerardinis, Gaudenz Danuser
Article
Biochemistry & Molecular Biology
Daniel J. Marston, Marco Vilela, Jaewon Huh, Jinqi Ren, Mihai L. Azoitei, George Glekas, Gaudenz Danuser, John Sondek, Klaus M. Hahn
NATURE CHEMICAL BIOLOGY
(2020)
Article
Biology
Xinxin Wang, Zhiming Chen, Marcel Mettlen, Jungsik Noh, Sandra L. Schmid, Gaudenz Danuser
Article
Cell Biology
Erik S. Welf, Christopher E. Miles, Jaewon Huh, Etai Sapoznik, Joseph Chi, Meghan K. Driscoll, Tadamoto Isogai, Jungsik Noh, Andrew D. Weems, Theresa Pohlkamp, Kevin Dean, Reto Fiolka, Alex Mogilner, Gaudenz Danuser
DEVELOPMENTAL CELL
(2020)
Article
Multidisciplinary Sciences
Madhura Bhave, Rosa E. Mino, Xinxin Wang, Jeon Lee, Heather M. Grossman, Ashley M. Lakoduk, Gaudenz Danuser, Sandra L. Schmid, Marcel Mettlen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Ning Zhang, Yanhui Li, Tsung-Po Lai, Jerry W. Shay, Gaudenz Danuser
Summary: Research indicates that as individuals age, telomeres shorten gradually and are involved in regulating gene expression through models like TPE and TPE-OLD. An imaging approach has been developed to systematically examine the occurrence of TPE-OLD at the single cell level, which is crucial for establishing TPE-OLD as a credible mechanism for gene expression regulation.
Article
Multidisciplinary Sciences
Jungsik Noh, Gaudenz Danuser
Summary: The study found that under-ascertainment of COVID-19 cases is universal, with actual cumulative cases estimated to be 5-20 times greater than confirmed cases in some countries. Their machine learning framework showed reliability in estimating the number of infections.
Article
Cell Biology
Christoph J. Burckhardt, John D. Minna, Gaudenz Danuser
Summary: The research demonstrates that SH3BP4 regulates integrin surface expression in a signaling-dependent manner, impacting cell-matrix interactions. Decreased Akt kinase activity leads to SH3BP4 mediating endocytosis of neuropilin-1 and alpha 5/beta 1-integrins, inhibiting cell spreading.
DEVELOPMENTAL CELL
(2021)
Article
Biology
Sangyoon J. Han, Evgenia Azarova, Austin J. Whitewood, Alexia Bachir, Edgar Guttierrez, Alex Groisman, Alan R. Horwitz, Benjamin T. Goult, Kevin M. Dean, Gaudenz Danuser
Summary: The study reveals the crucial role of concurrent recruitment of talin and vinculin in early integrin-based nascent adhesions, which is essential for the maturation of NAs. Specifically, the function of the R8 domain in talin is identified as important for NA maturation, affecting vinculin binding and recruitment rates.
Article
Biochemistry & Molecular Biology
Assaf Zaritsky, Andrew R. Jamieson, Erik S. Welf, Andres Nevarez, Justin Cillay, Ugur Eskiocak, Brandi L. Cantarel, Gaudenz Danuser
Summary: The study utilized a generative neural network and supervised machine learning to classify the metastatic efficiency of melanoma cells, revealing characteristic features of metastatic cells and validating these findings with live cell observations. It demonstrates how artificial intelligence can aid in identifying cell properties predictive of complex phenotypes and integrated cell functions that may be subtle and difficult for human experts to identify in raw imagery.
Article
Biochemical Research Methods
Christoph J. Burckhardt, John D. Minna, Gaudenz Danuser
Summary: Co-immunoprecipitation is widely used in studying protein-protein interactions, but requires considerable optimization, and results are often presented non-quantitatively. This protocol offers an optimized workflow using semi-quantitative immunoblot densitometry for increased reliability and reproducibility.
Article
Multidisciplinary Sciences
Claudia Schafer, Yawen Ju, Youngbin Tak, Cesar Vazquez, Sangyoon J. Han, Edwin Tan, Jerry W. Shay, Mats Holmqvist, Gaudenz Danuser, William M. Schopperle, Glenn Bubley