Article
Microbiology
A. E. Shea, A. E. Frick-Cheng, S. N. Smith, H. L. T. Mobley
Summary: This study demonstrates that phenotypic characteristics can be used to predict the severity of urinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains. These characteristics can help differentiate UPEC from other E. coli strains and reduce the overuse of antibiotics.
Article
Infectious Diseases
Alfredo Montes-Robledo, Rosa Baldiris-Avila, Johan Fabian Galindo
Summary: The study suggests that phenyl mannoside derivatives may serve as FimH inhibitors to mitigate urinary tract infections caused by UPEC. Molecular dynamic simulations indicated that the ligands showed high binding affinity and stability with the lectin domain of FimH proteins.
Article
Microbiology
Rongrong Wu, Ashok Kumar Kumawat, Isak Demirel
Summary: Urinary tract infections (UTIs) caused by uropathogenic E. coli (UPEC) are common in humans. Trimethylamine N-oxide (TMAO), a proinflammatory metabolite, has been linked to vascular inflammation and chronic kidney disease. This study investigated the effects of TMAO on UTIs and found that it aggravated the release of inflammatory mediators and enhanced UPEC colonization of bladder epithelial cells. These findings suggest a potential role of TMAO in infectious diseases, providing a basis for further research on the link between diet, gut microbiota, and UTIs.
Article
Microbiology
Ulises Hernandez-Chinas, Maria E. Chavez-Berrocal, Ricardo E. Ahumada-Cota, Armando Navarro-Ocana, Luz M. Rocha-Ramirez, Yolanda Perez-del Mazo, Maribel Alvarado-Cabello, Gabriel Perez-Soto, Luis A. Leon-Alamilla, Salvador E. Acevedo-Monroy, Diego Esquiliano, Atlantida M. Raya-Rivera, Carlos A. Eslava
Summary: The study suggests that autologous bacterial lysates (ABL) may have a therapeutic effect on children with recurrent urinary tract infections (rUTI) by controlling the infection for almost one year.
Article
Pharmacology & Pharmacy
Georgia I. Nasi, Konstantina I. Georgakopoulou, Marilena K. Theodoropoulou, Nikos C. Papandreou, Evangelia D. Chrysina, Paraskevi L. Tsiolaki, Vassiliki A. Iconomidou
Summary: Type I fimbriae, composed of four subunits, play a critical role in uropathogenic Escherichia coli (UPEC) infections, with the FimH adhesin at the fimbrial tip as the key component. We propose that the amyloidogenic potential of FimH can be used for developing therapeutic agents against Urinary Tract Infections (UTIs). By identifying aggregation-prone regions (APRs) and synthesizing corresponding peptide-analogues, we demonstrate that these molecules can interfere with FimH folding or compete for the mannose-binding pocket, making them promising antimicrobial candidates.
Article
Cell Biology
Ashok Kumar Kumawat, Geena Varghese Paramel, Kartheyaene Jayaprakash Demirel, Isak Demirel
Summary: The study showed that renal fibroblasts, but not renal epithelial cells, release IL-1 beta during a UPEC infection, which is dependent on factors like NLRP3, caspase-1, and caspase-4. Additionally, the UPEC virulence factor alpha-hemolysin was found to be necessary for IL-1 beta release.
Article
Public, Environmental & Occupational Health
Vanesa Garcia, Luz Leston, Ana Parga, Isidro Garcia-Menino, Javier Fernandez, Ana Otero, John E. Olsen, Ana Herrero-Fresno, Azucena Mora
Summary: This study investigated 31 strains of Escherichia coli from different sources using genomic comparison, bladder-cell, and biofilm formation assays. It found that animal-derived and human extraintestinal infection strains had high genomic similarity and exhibited similar pathogenic characteristics in cell and biofilm assays. These results highlight the potential role of food-producing animals as a source of urinary tract infections.
Article
Public, Environmental & Occupational Health
Ali Qasemi, Fateh Rahimi, Mohammad Katouli
Summary: The prevalence of biofilm and beta-lactamase-producing UPEC strains differed significantly between Isfahan and Zahedan in Iran, with higher rates observed in the city of Zahedan. This suggests that socioeconomic status and environmental factors may play a role in the pathogenicity of these strains.
PATHOGENS AND GLOBAL HEALTH
(2022)
Article
Immunology
Paulo A. Schueroff, Fabia A. Salvador, Cecilia M. Abe, Haleluya T. Wami, Eneas Carvalho, Rodrigo T. Hernandes, Ulrich Dobrindt, Tania A. T. Gomes, Waldir P. Elias
Summary: The study revealed a hybrid strain UPEC-46 with characteristics of both EAEC and UPEC, with a significant role of AFP adhesin in adherence to colorectal and bladder cell lines.
Article
Biotechnology & Applied Microbiology
Ali Qasemi, Fateh Rahimi, Mohammad Katouli
Summary: The study investigated the genetic diversity and virulence-associated genes of biofilm-producing UPEC strains isolated from patients with UTI in Iran. Strong BFP strains showed higher levels of VAGs, while weak BFP strains exhibited more pronounced presence of specific VAGs. There exists a high genetic diversity among the BFP strains with distinct profiles of virulence-associated genes.
INTERNATIONAL MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Katarina Mestrovic Popovic, Petra Povalej Brzan, Tomaz Langerholc, Natasa Marcun Varda
Summary: Probiotics may be helpful in alleviating UTI symptoms and preventing UTI recurrence.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Medicine, Research & Experimental
Manuel G. Ballesteros-Monrreal, Pablo Mendez-Pfeiffer, Edwin Barrios-Villa, Margarita M. P. Arenas-Hernandez, Yessica Enciso-Martinez, Cesar O. Sepulveda-Moreno, Enrique Bolado-Martinez, Dora Valencia
Summary: This study systematically reviewed published reports on the virulence genes, antibiotic resistance, and phylogenetic groups of uropathogenic Escherichia coli (UPEC) in the Mexican population. The findings revealed that multidrug-resistant UPEC strains are highly prevalent in Mexico, reducing the therapeutic options available. This has significant implications for the diagnosis and treatment of urinary tract infections.
ARCHIVES OF MEDICAL RESEARCH
(2023)
Article
Microbiology
Ivana Kerkez, Paul M. Tulkens, Tanel Tenson, Francoise Van Bambeke, Marta Putrins
Summary: Research shows that commonly used antibiotics for urinary tract infections are much less effective against intracellular UPEC compared to extracellular UPEC. Except fluoroquinolones, most antibiotics were unable to achieve a bactericidal effect intracellularly at clinically achievable concentrations. Fluoroquinolones ciprofloxacin and finafloxacin killed almost all extracellular bacteria at concentrations close to the MIC, but significantly higher concentrations were needed for intracellular bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Pharmacology & Pharmacy
Leila Mousavifar, Meysam Sarshar, Clarisse Bridot, Daniela Scribano, Cecilia Ambrosi, Anna Teresa Palamara, Gerard Vergoten, Benoit Roubinet, Ludovic Landemarre, Julie Bouckaert, Rene Roy
Summary: A small library of FimH antagonists based on previously described C-linked allyl alpha-D-mannopyranoside was synthesized. Two new members with sub nanomolar affinity were identified and shown to have improved binding compared to previously published analogs. The crystal structure of FimH co-crystallized with one of the antagonists revealed its binding mode, and the antagonists were effective in decreasing bacterial adhesion to human bladder epithelial cells.
Article
Microbiology
Patrick da Silva, Bruna C. Lustri, Ivana Giovannetti Castilho, Adriano Martison Ferreira, Rodrigo T. Hernandes, Mark A. Schembri, Cristiano G. Moreira
Summary: This study reports the draft genome sequence of 9 fluoroquinolone-resistant UPEC isolates from Brazil, analyzing their antimicrobial resistance profile, phylogroup, sequence type, virulence genes, and resistance marks. The isolates were found to exhibit resistance to other antibiotics besides fluoroquinolones, with a high prevalence of virulence genes detected.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Neda Moazzezy, Elham Rismani, Maryam Rezaei, Mohammad Reza Asadi Karam, Sima Rafati, Saeid Bouzari, Mana Oloomi
Summary: The development of bacterial resistance to antibiotics has led to the attention of antimicrobial peptides (AMPs). This study computationally investigated the interaction between derived peptides and bacterial membrane and found that the derived peptides could exhibit similar antimicrobial effects to HNP-1. Furthermore, chemical modifications improved the physicochemical properties of the peptides and retained similar binding affinity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Meeting Abstract
Infectious Diseases
M. Pooya, M. Saleh, A. Abd, H. Mazaheri, E. Khosravi, A. Abdollahi, M. Mardani, S. Bouzari
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Biochemistry & Molecular Biology
Hedyeh Sharbatdaralaei, Mohammad Reza Asadi Karam, Khadijeh Ahmadi, Mehri Habibi
Summary: This study aimed to design and express a hybrid protein composed of PcrV and ExoS from P. aeruginosa and evaluate its immunogenicity in patients with UTI. The hybrid protein ExoS.PcrV showed potential immunological advantages and has been validated to interact with immune receptors through initial experiments.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Immunology
Anahita Rahimkhani, Setareh Haghighat, Hassan Noorbazargan, Mehdi Mahdavi
Summary: The new HBsAg vaccine formulated with alpha-tocopherol enhances specific total IgG responses and affects immunologic cytokines depending on the dose of alpha-tocopherol used in the vaccine formulation.
MICROBIAL PATHOGENESIS
(2021)
Article
Immunology
Arezou Rezaei, Ghorbanali Shahabi, Sobhan Faezi, Mehdi Shafiee Ardestani, Hedayatollah Shirzad, Kayhan Azadmanesh, Ebrahim Mirzajani, Arezoo Shajiei, Mehdi Mahdavi
Summary: This study investigated new strategies to enhance the immune response to HIV-1 vaccine using immunological adjuvants. The results showed that conjugating HIV-1 p24-Nef with type-A flagellin significantly increased lymphocyte proliferation and cytokine production, leading to improved humoral and cellular immune responses. Furthermore, the route of vaccine administration also affected the immune response.
Article
Immunology
Yasaman Eshraghi, Yasaman Vahdani, Pegah Karimi, Meghdad Abdollahpour-Alitappeh, Asghar Abdoli, Morteza Taghizadeh, Mehdi Mahdavi
Summary: This study found that adding alpha-tocopherol to the influenza vaccine can increase immune response in the elderly, improving the effectiveness of the vaccine.
Article
Microbiology
Aida Haji Hossein Tabrizi, Mehri Habibi, Fatemeh Foroohi, Taher Mohammadian, Mohammad Reza Asadi Karam
Summary: This study evaluated the antimicrobial effects of peptide IDR1018 and chitosan nanoparticles (CNs) against ciprofloxacin-resistant uropathogenic Escherichia coli (UPEC). The results showed that peptide IDR1018 and CNs have antimicrobial properties on UPEC isolates and can inhibit and eradicate biofilms in a concentration-dependent manner.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Immunology
Shaghayegh Shahkolahi, Pegah Shakibnia, Shahla Shahbazi, Samira Sabzi, Farzad Badmasti, Mohammad Reza Asadi Karam, Mehri Habibi
Summary: In this study, 200 Klebsiella pneumoniae isolates were collected from patients with urinary tract infections in Tehran, Iran. The isolates were analyzed for antibiotic resistance, ESBLs, AmpCs, integron genes, plasmids, and genomic relatedness. The results showed high levels of resistance against ampicillin, with most isolates carrying beta-lactamase genes and the most prevalent integron being class I. The study also identified the replicons of plasmids and the ability of selected isolates to transfer genes. Finally, the isolates were categorized into pulsotypes and MLST identified sequence types.
ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
(2022)
Article
Immunology
Melika Haghighi, Akbar Khorasani, Pegah Karimi, Rouhollah Keshavarz, Mehdi Mahdavi
Summary: In this study, different formulations of the inactivated SARS-CoV-2 virus were developed with different adjuvants, and the immune responses were assessed. The results showed that Montanide 51VG and Montanide ISA720VG adjuvants were more effective in inducing immune responses compared to Alum adjuvant.
Article
Oncology
Zahra Pourramezan, Fatemeh Akhavan Attar, Maryam Yusefpour, Masoumeh Azizi, Mana Oloomi
Summary: This study used qRT-PCR to measure the expression levels of lncRNAs in breast cancer patients and healthy women. The results showed that certain circulating lncRNAs were down-regulated in breast cancer patients, while others were up-regulated. SPRY4-IT1, XIST, and H19 lncRNAs showed excellent potential in distinguishing breast cancer from healthy controls. Furthermore, H19 and SPRY4-IT1 lncRNAs were found to be significantly correlated with clinicopathological traits in breast cancer patients.
Article
Biotechnology & Applied Microbiology
Mojtaba Heydari, Mehdi Kalani, Younes Ghasemi, Mahmood Nejabat
Summary: This study evaluated the efficacy and safety of Latilactobacillus sakei as an ophthalmic bacterial lysate or oral probiotic on patients with dry eye disease (DED). Results showed that the ophthalmic drops significantly decreased DED signs and symptoms, while the oral capsules had no effect. The ophthalmic formulation containing L. sakei lysate improved DED and suppressed ocular surface inflammatory response.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Immunology
Somayeh Kalanaky, Saideh Fakharzadeh, Pegah Karimi, Maryam Hafizi, Hamidreza Jamaati, Seyed Mehdi Hassanzadeh, Akbar Khorasani, Mehdi Mahdavi, Mohammad Hassan Nazaran
Summary: Nanoadjuvants developed using Nanochelating Technology can enhance the potency of alum-adjuvanted SARS-CoV-2 vaccines by inducing stronger cellular immune responses and improving humoral immune responses simultaneously.
Article
Immunology
Aref Sepasi, Mehri Ghafourian, Morteza Taghizadeh, Mehdi Mahdavi
Summary: In this study, the expression and potency of a recombinant H1N1 hemagglutinin (HA) vaccine were evaluated in a mouse model. The vaccine, produced in an SF9 cell line and formulated in MF59 adjuvant, showed a robust immune response and cytotoxic T lymphocyte (CTL) activity compared to an alum-based vaccine and phosphate-buffered saline (PBS). Additionally, the MF59-based vaccine demonstrated superior humoral immune responses and increased survival rates in comparison to the alum-based vaccine. The MF59 formulation seems to contribute to the superiority of the candidate vaccine.
Article
Biochemistry & Molecular Biology
Maryam Javadi, Mana Oloomi, Saeid Bouzari
Summary: Immunoinformatics or vaccine informatics focuses on applying computational approaches to advance vaccine research and development. This study aimed to design a cross-reactive epitope vaccine to stimulate CD8 + and CD4 + T cells against UTI pathogens. Predictions based on informatics were used to design potentially cross-protective T-cells inducing vaccine candidates.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Article
Multidisciplinary Sciences
Mana Oloomi, Neda Moazzezy, Saeid Bouzari
