期刊
MOLECULAR IMMUNOLOGY
卷 50, 期 3, 页码 172-176出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2011.11.010
关键词
FDCs (follicular dendritic cells); BAFF (B cell activating factor TNF family)
资金
- Ministry of Education, Science and Culture of Japan
- National Institute of Biomedical Innovation (NIBIO)
- Grants-in-Aid for Scientific Research [21249026, 23592664, 22021006, 23390091, 09J00662, 21390481, 22390073] Funding Source: KAKEN
Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of naive mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for similar to 0.2% of the spleen cells of naive mice, were CD45(-), FDC-M2(+), and ICAM-1(+), and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation. (c) 2011 Elsevier Ltd. All rights reserved.
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