Article
Oncology
Jake G. Maule, Lani K. Clinton, Ryon P. Graf, Jinpeng Xiao, Geoffrey R. Oxnard, Jeffrey S. Ross, Richard S. P. Huang
Summary: This study analyzed 418 tumor specimens using 22C3, 28-8, and SP142 PD-L1 IHC assays and found that 22C3 is the most sensitive assay. This finding can assist clinical teams in determining which PD-L1 IHC assay to use for each patient.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Jonathan Schupp, Arne Christians, Niklas Zimmer, Lukas Gleue, Helmut Jonuleit, Mark Helm, Andrea Tuettenberg
Summary: The presence and interaction of immune cells in the tumor microenvironment have a significant impact on disease progression and treatment response. Spatial analyses are crucial in the complex interplay between neoplastic and immune cells, aiming to develop new therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kun-Hao Bai, Yi-Yang Zhang, Xue-Ping Li, Xiao-Peng Tian, Meng-Meng Pan, Da-Wei Wang, Yu -Jun Dai
Summary: In this study, the expression of TNFAIP8L2 was explored across various tumor types and found to be highly expressed in most tumor types and correlated with prognosis. TNFAIP8L2 expression predicted improved survival in certain tumors, while poorer survival in others. Furthermore, TNFAIP8L2 was significantly associated with cancer stem cell index and increased immune cell infiltration. Therefore, TNFAIP8L2 may serve as a novel immune checkpoint biomarker across different tumor types and could be a potential target for immunotherapy.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Immunology
Hui-Bo Du, Sun-Ban Jiang, Zhen-Ao Zhao, Hong Zhang, Li-Min Zhang, Zhao Wang, Ya-Xiong Guo, Jia-Yi Zhai, Peng Wang, Zi-Gang Zhao, Chun-Yu Niu, Li-Na Jiang
Summary: The study found that TIPE2 improves PHSML-induced CD4(+) T cell dysfunction by regulating the TLR2/TLR4 signaling pathway, providing a new intervention target for immune dysfunction following hemorrhagic shock.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hiroyuki Suzuki, Kazuki Ozawa, Tomohiro Tanaka, Mika K. Kaneko, Yukinari Kato
Summary: CD44 is investigated as a cancer stem cell marker in tumor progression. Different splicing variants are overexpressed in many cancers and play critical roles in tumor metastasis, acquisition of stem cell properties, and resistance to treatments. Therefore, understanding the functions and distribution of each CD44 variant is important for novel tumor diagnosis and therapy.
Article
Biochemistry & Molecular Biology
Pavel Khramtsov, Artem Minin, Zarina Galaeva, Elena Mukhlynina, Maria Kropaneva, Mikhail Rayev
Summary: This paper highlights the importance of optimizing the composition of substrates for nanozymes. By altering the composition of the substrate buffer, including buffer species and additives, the catalytic activity and analytical signal of nanozymes can be significantly enhanced. The study emphasizes the need to prioritize substrate composition optimization in the development of nanozyme-based assays to improve reaction efficiency.
Article
Neurosciences
Anne-Kathrin Lutz, Helen Friedericke Bauer, Valentin Ioannidis, Michael Schoen, Tobias M. Boeckers
Summary: This study validated a set of commercial and homemade SHANK3 antibodies in Western Blotting, synaptic immunocytochemistry, and immunohistochemistry, and found that only a small subset of the antibodies met the criteria of quality and specificity. The study highlights the importance of antibody specificity testing in the research field.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2022)
Article
Immunology
Ana Carolina Matias Dinelly Pinto, Rodolfo de Melo Nunes, Igor Albuquerque Nogueira, Bernhard Fischer, Rudolf Lucas, Virginia Claudia Carneiro Girao-Carmona, Vivian Louise Soares de Oliveira, Flavio Almeida Amaral, Georg Schett, Francisco Airton Castro Rocha
Summary: TNF inhibitors are commonly used for the treatment of arthropathies like rheumatoid arthritis (RA), but not all patients achieve remission and there is a risk of infection. This study found that the lectin-like domain of TNF has anti-inflammatory effects and can mitigate the development of immune-mediated arthritis in mice.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Denys Rujchanarong, Julia Lefler, Janet E. Saunders, Sarah Pippin, Laura Spruill, Jennifer R. Bethard, Lauren E. Ball, Anand S. Mehta, Richard R. Drake, Michael C. Ostrowski, Peggi M. Angel
Summary: This study introduces a novel approach that combines immunohistochemistry with extracellular matrix imaging mass spectrometry to analyze ECM proteins based on patterns of cell types in tissue. The research also delves into the regulation and expression of collagen within breast stroma, shedding light on its role in cancer progression.
Article
Medicine, General & Internal
Wang Yi-Wen, Li Long-Long, Lu Ming, Li Hao, Hu Kong-Wang
Summary: The presence of circulating tumor cells (CTCs) and CD44-positive CTCs is significantly associated with the aggressiveness, metastasis, and recurrence of gastric cancer. Furthermore, the presence of CD44-positive CTCs is an independent predictor of gastric cancer recurrence.
ARCHIVES OF MEDICAL SCIENCE
(2022)
Article
Oncology
Caiyun Wang, Xiaodong Zhao, Haibin Zhang, Zhitong Bing, Yu Wu, Rui Li, Yongxiu Yang, Kehu Yang
Summary: This study identified 3 genes as signatures and potential biomarkers for unexplained infertility. These genes could guide immunotherapy for these patients and become new treatment targets.
ANNALS OF TRANSLATIONAL MEDICINE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yanmei DU, Xiaohua Liu, Changjie Xiao, Jianbin LI, Zhenxian Sheng, Yuxin Wang, Ronglin Wang, Xijiao Yu
Summary: The roles and molecular mechanisms of TIPE2 in periodontitis were investigated. The expression of TIPE2 and NF-κB p65 was found to be increased in gingival tissues of rat periodontitis. Knockdown of TIPE2 upregulated NF-κB signaling pathway activity. Therefore, increasing TIPE2 may be a potential therapeutic approach for periodontitis.
JOURNAL OF APPLIED ORAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Eman I. Sobeh, Gamal El-ghannam, Reda M. S. Korany, H. M. Saleh, Souad A. Elfeky
Summary: This study reports the preparation of CUR/HA nanocomposite and its application in protecting male Wistar rats from the carcinogenic consequences of gamma irradiation. TEM and XRD analysis provided information on the morphology and structure of the nanosized HA nanoparticles and CUR/HA nanocomposite. Biochemical analysis and histological examinations demonstrated that CUR/HA nanocomposite injection did not significantly affect non-irradiation rats, but controlled the changes in pro-inflammatory cytokines, kidney function, and liver function when injected pre-irradiation. This research introduces a novel effective protection modality for the gamma irradiation hazard.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Cell Biology
Rui Yao, Lingyao Kong, Chunlei Yang, Jiaqi Du, Guojun Zhao, Yapeng Li
Summary: This study demonstrates that TIPE2 inhibits cardiac hypertrophy by targeting TLR4 and repressing macrophage activation.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Chemistry, Medicinal
Ahmad Nassar, Abed N. Azab
Summary: This study investigates the efficacy of dexamethasone and pentoxifylline in behavioral models in rats with mood disorders. The results show that pentoxifylline has antidepressant-like and anti-manic-like effects, accompanied by a reduction in pro-inflammatory mediator levels in the brain. Pentoxifylline may potentially be a therapeutic intervention for mood disorders.
Article
Immunology
Yu Song, Peishan Li, Liping Qin, Zhiliang Xu, Baichun Jiang, Chunhong Ma, Changshun Shao, Yaoqin Gong
Summary: CUL4B functions as an intrinsic negative regulator of the TLR-triggered inflammatory response, enhancing proinflammatory cytokine production and reducing anti-inflammatory cytokine IL-10 production when deleted in macrophages. Enhanced TLR-induced inflammatory responses in the absence of CUL4B are mediated by increased GSK3β activity. By regulating the PI3K-AKT-GSK3β pathway, CUL4B restricts TLR-triggered inflammatory responses.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Hematology
Xin Li, Zi Sheng, Yuanxin Sun, Yuanjian Wang, Miao Xu, Zhiyue Zhang, Hui Li, Linlin Shao, Yanqi Zhang, Jinming Yu, Chunhong Ma, Chengjiang Gao, Ming Hou, Heyu Ni, Jun Peng, Ji Ma, Qi Feng
Summary: The expression of HLA-G and immunoglobulin-like transcripts is found to be impaired in patients with ITP. Recombinant HLA-G can correct this abnormality by upregulating immunoglobulin-like transcripts, stimulating Th2 differentiation, and downregulating Th1 and Th17 immune responses, suggesting that HLA-G can be a potential diagnostic marker and therapeutic option for ITP.
Article
Immunology
Yunyun Guo, Fei Jiang, Lingli Kong, Haifeng Wu, Honghai Zhang, Xiaorong Chen, Jian Zhao, Baoshan Cai, Yanqi Li, Chunhong Ma, Fan Yi, Lei Zhang, Bingyu Liu, Yi Zheng, Lingqiang Zhang, Chengjiang Gao
Summary: STING is a critical adaptor protein for innate antiviral and antitumor immunity, regulated by protein ubiquitination, with OTUD5 identified as a key deubiquitinase that interacts with STING to maintain its stability. Knockout of OTUD5 resulted in impaired immune responses and increased susceptibility to viral infection and tumor development.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Cell Biology
Baoshan Cai, Jian Zhao, Yuling Zhang, Yaxing Liu, Chunhong Ma, Fan Yi, Yi Zheng, Lei Zhang, Tian Chen, Huiqing Liu, Bingyu Liu, Chengjiang Gao
Summary: USP5 acts as a key scaffold protein in recruiting the E3 ligase MARCHF7 for autophagic degradation of NLRP3 independently of its deubiquitinating enzyme activity. Knockdown of USP5 promotes inflammatory signaling release, while overexpression has inhibitory effects.
Letter
Immunology
Chaojia Chen, Jiali Peng, Shuaiya Ma, Yuming Ding, Tao Huang, Shigang Zhao, Lifen Gao, Xiaohong Liang, Chunyang Li, Chunhong Ma
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Medical Laboratory Technology
Zhufeng Chen, Wanju Xu, Wanshan Ma, Xiaohong Shi, Shuomin Li, Mingju Hao, Yuanxun Fang, Li Zhang
Summary: COVID-19, caused by SARS-CoV-2, is a highly infectious disease where clinical laboratory detection plays a crucial role. Nucleic acid amplification testing and monitoring specific antibodies are effective for diagnosis and disease progression evaluation. Various abnormal clinical laboratory indicators can signal disease severity and progression.
CLINICA CHIMICA ACTA
(2021)
Article
Immunology
Siyu Tan, Xiaowei Guo, Mengzhen Li, Tixiao Wang, Zehua Wang, Chunyang Li, Zhuanchang Wu, Nailin Li, Lifen Gao, Xiaohong Liang, Chunhong Ma
Summary: The study identifies Zhx2 as a novel negative regulator that restricts NK cell maturation and function, with its loss enhancing NK cell survival and response to IL-15. Zhx2 deficiency in NK cells inhibits tumor growth and metastasis, highlighting its therapeutic potential for enhancing NK cell-mediated tumor surveillance.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Cell Biology
Shuai Tan, Junhao Zhang, Yang Sun, Anton Gistera, Zi Sheng, Rickard E. Malmstrom, Ming Hou, Jun Peng, Chunhong Ma, Wangjun Liao, Nailin Li
Summary: Platelets enhance Th1 and Treg cell responses of CD4(+) central memory T cells through PF4-dependent mitochondrial biogenesis and proliferation of Tcm cells.
Article
Multidisciplinary Sciences
Jinxiu Hou, Lulu Han, Ze Zhao, Huiqing Liu, Lei Zhang, Chunhong Ma, Fan Yi, Bingyu Liu, Yi Zheng, Chengjiang Gao
Summary: Research demonstrates that deubiquitinase USP18 regulates MAVS-mediated antiviral signaling by promoting MAVS interaction with TRIM31, leading to increased production of type I interferon and enhanced resistance to RNA viruses.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Tixiao Wang, Jie Zhang, Na Li, Mengzhen Li, Shuaiya Ma, Siyu Tan, Xiaowei Guo, Zehua Wang, Zhuanchang Wu, Lifen Gao, Chunhong Ma, Xiaohong Liang
Summary: The spatial organization of immune cells within the tumor microenvironment (TME) plays a crucial role in anti-tumor immunity, tumor progression, and therapeutic response. Tim-3 is an immune checkpoint with potential as an immunotherapy target. This study examined the spatial distribution of Tim-3/Tim-3L in the TME and found that CD4 T cells were more accumulated in the tumor region and had a closer association with patient survival. CD4 T cells surrounding Tim-3L(+) cells expressed higher levels of Tim-3. Depletion of CD4 T cells abrogated the inhibition of tumor growth and metastasis mediated by Tim-3 blockade. These findings highlight the importance of CD4 T cells in Tim-3/Tim-3L-mediated immunosuppression and provide insights for Tim-3 targeted cancer immunotherapy.
Article
Chemistry, Multidisciplinary
Yang Sun, Yan Teng, Liyuan Wang, Zhaoying Zhang, ChaoJia Chen, Yingchun Wang, Xiaodong Zhang, Peng Xiang, Xiaojia Song, Jinghui Lu, Nailin Li, Lifen Gao, Xiaohong Liang, Yuchen Xia, Zhuanchang Wu, Chunhong Ma
Summary: This study identifies LINC01431 as a novel host restriction factor for HBV transcription. It competitively binds with PRMT1 to inhibit the ubiquitination and degradation of PRMT1 mediated by HBx, thus repressing cccDNA transcription. In turn, HBV transcriptionally suppresses LINC01431 expression by inhibiting the transcription factor ZHX2.
Letter
Oncology
Yuming Ding, Chaojia Chen, Shuaiya Ma, Xue Sheng, Fangcheng Zhao, Jiali Peng, Haoqing Dong, Chunhong Ma, Chunyang Li
CANCER COMMUNICATIONS
(2022)
Letter
Biochemistry & Molecular Biology
Zhuanchang Wu, Xiaobo Lei, Xin Wang, Zhaoying Zhang, Yuming Li, Lifen Gao, Xiaohong Liang, Peihui Wang, Jianwei Wang, Chunhong Ma
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Immunology
Wanxin Zhuang, Lei Zhang, Yi Zheng, Bingyu Liu, Chunhong Ma, Wei Zhao, Suxia Liu, Feng Liu, Chengjiang Gao
Summary: Inflammasomes are crucial for the innate immune system, and dysregulation of their activation can have negative impacts on human health. This study reveals that USP3 acts as a key regulator of ASC ubiquitination, enhancing its stability and promoting inflammasome activation.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Letter
Biochemistry & Molecular Biology
Zhuanchang Wu, Zhaoying Zhang, Xin Wang, Jing Zhang, Caiyue Ren, Yuming Li, Lifen Gao, Xiaohong Liang, Peihui Wang, Chunhong Ma
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)