Article
Immunology
Murielle Golomingi, Jessie Kohler, Lorenz Jenny, Elaissa T. Hardy, Jozsef Dobo, Peter Gal, Gabor Pal, Bence Kiss, Wilbur A. Lam, Verena Schroeder
Summary: In this study, the role of MBL and MASP-1 in the haemostatic response after mechanical vessel injury was investigated using a microvascular bleeding model. MBL was found to recognize the injury site, while MASP-1 increased fibrin formation, platelet activation, and shortened bleeding time. These findings suggest that the complement lectin pathway has a beneficial role in the physiological coagulation response by supporting the crucial haemostatic system.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Hematology
Marco Mannes, Arthur Dopler, Oliver Zolk, Sophia J. Lang, Rebecca Halbgebauer, Britta Hoechsmann, Arne Skerra, Christian K. Braun, Markus Huber-Lang, Hubert Schrezenmeier, Christoph Q. Schmidt
Summary: Use of C5 inhibitor eculizumab to block the terminal complement pathway has revolutionized clinical management of complement-mediated diseases. New findings show that C3/C5 convertases function differently than previously thought, with C3 inhibition not necessarily leading to C5 inhibition. Surface-deposited C4b alone can recruit and activate C5, and only C5 bound on C3b/C4b-decorated surfaces requires surface fixation for activation.
Article
Immunology
Aleksandra E. Matusiak, Jan Stepniak, Andrzej Lewinski, Malgorzata Karbownik-Lewinska
Summary: Hypopituitarism in adults is associated with a decrease in blood concentration of mannan-binding lectin, which does not occur in hypopituitary patients on appropriate hormone replacement therapies. Therefore, measuring mannan-binding lectin levels may contribute to optimizing hormone replacement therapy doses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
G. H. Dorflinger, P. H. Hoyem, E. Laugesen, J. A. ostergaard, K. L. Funck, R. Steffensen, P. L. Poulsen, T. K. Hansen, M. Bjerre
Summary: Contrary to T1D, T2D is not per se associated with increased MBL serum level for a given MBL genotype or with diabetes duration. Serum MBL was inversely correlated with body fat percentage, and T2D patients with the high MBL expression genotype presented with deterioration of renal function.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Roberta Bulla, Lucrezia Rossi, Giovanni Furlanis, Chiara Agostinis, Miriam Toffoli, Andrea Balduit, Alessandro Mangogna, Marco Liccari, Giorgia Morosini, Uday Kishore, Paolo Manganotti
Summary: Brain fog, a constellation of neurological and psychiatric symptoms, can occur in the post-acute phase of COVID-19. It is characterized by inattention, short-term memory loss, and reduced mental acuity, which can impact cognition, concentration, and sleep. This study found that levels of Mannan-binding lectin (MBL), an important component of the complement system, were significantly lower in COVID-19 patients experiencing brain fog compared to recovered patients. These findings suggest that MBL deficiency may contribute to the development of long COVID-associated brain fog.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Yunzhi Liu, Na Zhao, Qishan Xu, Fan Deng, Ping Wang, Lijun Dong, Xiao Lu, Lihua Xia, Mingyong Wang, Zhengliang Chen, Jia Zhou, Daming Zuo
Summary: This study found that MBL limits Th17 immunity in silicosis patients by controlling the AhR/STAT3 pathway, providing new insight into the role of MBL deficiency in silicosis and other inflammatory diseases.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Immunology
Federica Defendi, Corentin Leroy, Olivier Epaulard, Giovanna Clavarino, Antoine Vilotitch, Marion Le Marechal, Marie-Christine Jacob, Tatiana Raskovalova, Martine Pernollet, Audrey Le Gouellec, Jean-Luc Bosson, Pascal Poignard, Matthieu Roustit, Nicole Thielens, Chantal Dumestre-Perard, Jean-Yves Cesbron
Summary: The study analyzed the complement activation pathways and components in COVID-19 patients, identifying two distinct patient clusters: one with activation of alternative and lectin pathways, low antigenic levels of complement components, and higher mortality and need for oxygen or ICU care; the other with high classical pathway activity, higher antigenic levels of complement components, lower need for ICU care, and no deaths.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Kristian Juul-Madsen, Anne Troldborg, Thomas R. Wittenborn, Mads G. Axelsen, Huaying Zhao, Lasse H. Klausen, Stefanie Luecke, Soren R. Paludan, Kristian Stengaard-Pedersen, Mingdong Dong, Holger J. Moller, Steffen Thiel, Henrik Jensen, Peter Schuck, Duncan S. Sutherland, Soren E. Degn, Thomas Vorup-Jensen
Summary: Nanotechnology allows for the study of single biomacromolecules, and research has shown that the superoligomeric structure of MBL forms nanoparticles with DNA in human plasma. These oligomers are correlated with disease activity in patients with systemic lupus erythematosus, and their size is connected to endothelial inflammation. Insights from an animal model of lupus suggest that DNA-stabilized superoligomers play a role in the pathobiology of autoimmune diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Lisa Hurler, Agnes Szilagyi, Federica Mescia, Laura Bergamaschi, Blanka Mezo, Gyorgy Sinkovits, Marienn Reti, Veronika Mueller, Zsolt Ivanyi, Janos Gal, Laszlo Gopcsa, Peter Remenyi, Beata Szathmary, Botond Lakatos, Janos Szlavik, Ilona Z. Bobek, Zita Prohaszka, Zsolt Forhecz, Dorottya Csuka, Erika Kajdacsi, Laszlo Cervenak, Petra Kiszel, Tamas Masszi, Istvan Valyi-Nagy, Reinhard A. Wuerzner, Paul Lyons, Erik J. M. Toonen, Zoltan Prohaszka
Summary: The lectin pathway is activated in acute COVID-19, but the role of MBL in COVID-19 is still not fully understood.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Mahdi Naseri, Maisha Maliha, Mostafa Dehghani, George P. Simon, Warren Batchelor
Summary: The development of a biosensor using MBL protein and PSM microparticles for detecting bacterial cells in water samples has been achieved. The biosensor shows a binary response, remaining blue in the presence of bacterial cells and changing color from blue to colorless in their absence.
Article
Hematology
Adrienn Teraz-Orosz, Magdalena Gierula, Anastasis Petri, David Jones, Renos Keniyopoullos, Patricia Badia Folgado, Salvatore Santamaria, James T. B. Crawley, David A. Lane, Josefin Ahnstrom
Summary: Protein S is a cofactor that enhances the inhibition of factor XIII activity and generation by TFPI alpha. The interaction between TFPI alpha and protein S is critical for this enhancement, and binding of C4BP to protein S LG1 abolishes the TFPI cofactor function. The amino acids involved in TFPI alpha enhancement and the mechanisms behind the reduced TFPI cofactor function of C4BP-bound protein S were investigated. It was found that Lys255, Glu257, Asp287, Arg410, Lys423, and Glu424 of protein S are critical for its function, and binding of the C4BP beta-chain blocks this function.
Article
Biochemistry & Molecular Biology
Tailin He, Bo Zhou, Guohuan Sun, Qinnan Yan, Sixiong Lin, Guixing Ma, Qing Yao, Xiaohao Wu, Yiming Zhong, Donghao Gan, Shaochuan Huo, Wenfei Jin, Di Chen, Xiaochun Bai, Tao Cheng, Huiling Cao, Guozhi Xiao
Summary: The novel Pinch-Cxcl12-Mbl2 signaling pathway promotes the interactions between bone and liver to modulate immunity and hematopoiesis, which may provide a useful therapeutic target for immune and infectious diseases.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Florent Le Guern, Anne Gaucher, Gina Cosentino, Marion Lagune, Henk P. P. Haagsman, Anne-Laure Roux, Damien Prim, Martin Rottman
Summary: Establishing rapid and accurate diagnosis of sepsis is crucial for improving clinical outcomes. Analytical platforms that can quickly detect pathogen-associated molecular patterns (PAMPs) in blood offer a powerful host-independent biomarker for sepsis. This study investigated a novel concept of using pre-bound and fluorescent ligands released from lectins in contact with high-affinity ligands to create precise avidity fluorescent ligands. Chemical modifications of these ligands showed differences in their interactions with mannan-binding lectin (MBL) and human surfactant protein D (SP-D), indicating the potential for diagnostic tool development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Jialiang Luo, Lei Li, Bo Chang, Zhengyumeng Zhu, Fan Deng, Mengyao Hu, Yu Yu, Xiao Lu, Zhengliang Chen, Daming Zuo, Jia Zhou
Summary: This study reveals the important role of the interaction between MBL and hepatic stellate cells in controlling the progression of liver fibrosis. MBL can promote senescence in hepatic stellate cells by interacting with cell surface receptors, exacerbating liver fibrosis. Restoring MBL expression in the liver or eliminating senescent cells can inhibit the development of liver fibrosis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Multidisciplinary Sciences
Li Xu, Honglian Jiang, Jingwen Xie, Qishan Xu, Jia Zhou, Xiao Lu, Mingyong Wang, Lijun Dong, Daming Zuo
Summary: MBL deficiency worsens renal fibrosis in a chronic kidney disease mouse model, and macrophages play a crucial role in MBL-mediated suppression of renal fibrosis. MBL inhibits macrophage-to-myofibroblast transition by suppressing matrix metalloproteinase 9 production and Akt signaling activation.