Article
Immunology
Floriana Mulas, Xu Wang, Shanshan Song, Gopala Nishanth, Wenjing Yi, Anna Brunn, Pia-Katharina Larsen, Berend Isermann, Ulrich Kalinke, Antonio Barragan, Michael Naumann, Martina Deckert, Dirk Schlueter
Summary: OTUB1 is identified as a potent novel regulator of DCs during infectious and inflammatory diseases. It promotes NF-kappa B activity, regulates cytokine production, and impacts the response to pathogens.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
Tianzhuo Zhang, Qianwen Shi, Huining Gu, Biaoyi Yu, Sha Yin, Qing Ge, Xiaoning Mo, Xiaofeng Liu, Jing Huang
Summary: CCDC134 is a cytokine-like molecule that serves as a positive regulator of TCR-proximal signaling by interacting with CD3e and modulating its ubiquitination and degradation. T cell-specific deletion of Ccdc134 results in decreased mature CD4(+) and CD8(+) T cells, impaired T cell homeostasis, and attenuated response to TCR stimulation in vitro and in vivo. These findings highlight the importance of CCDC134 in T cell development and function, and its role in T cell-mediated inflammatory and antitumor responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yulin Du, Yifan Lyu, Jie Lin, Chunran Ma, Qiang Zhang, Yutong Zhang, Liping Qiu, Weihong Tan
Summary: We manipulated the intermembrane distance between antigen-presenting cells (APCs) and T cells using DNA nanostructures of different sizes. Our results show that the axial distance of the contact zone is critical for T-cell activation, likely through modulating protein reorganization and mechanical force.
NATURE NANOTECHNOLOGY
(2023)
Article
Cell Biology
Yuhui Yang, Hao Liu, Ruoxi Wang, Yi Zhao, Yunfei Zheng, Yiping Huang, Weiran Li
Summary: Repairing orthodontic external root resorption and periodontal tissue dysfunction caused by mechanical force is difficult in clinical practice. Cementoblasts play a vital role in cementum mineralization, which is important for restoring damaged cementum. The underlying mechanism of how autophagy mediates cementoblast mineralization is unclear. In this study, we found that murine cementoblasts exhibit compromised mineralization under compressive force. Autophagy is indispensable for cementoblast mineralization and activating it can reverse cementoblast mineralization and prevent cementum damage during tooth movement. We also identified periostin as a mediator of autophagy and mineralization in cementoblasts. Silencing periostin significantly inhibited cementoblast mineralization and suppressed Wnt signaling by modulating the stability of β-catenin. These findings highlight the importance of autophagy in cementoblast mineralization and provide a potential strategy for cementum remineralization and periodontal tissue regeneration under compressive force.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jesse Goyette, David Depoil, Zhengmin Yang, Samuel A. Isaacson, Jun Allard, P. Anton van der Merwe, Katharina Gaus, Michael L. Dustin, Omer Dushek
Summary: Protein-protein binding domains, such as the Src homology 2 (SH2) domains, play a critical role in signaling networks. Tandem SH2 domains can enhance binding affinity and specificity, but there is a trade-off between long-lived binding and rapid signaling reversal. This study shows that phosphatases can accelerate the unbinding rate of tandem SH2 domains, breaking the trade-off and allowing for efficient T cell antigen discrimination.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Dapu Liu, Xiaoxing Zhang, Qingliang Li, Yunhua Xiao, Guoxia Zhang, Wenchao Yin, Mei Niu, Wenjing Meng, Nana Dong, Jihong Liu, Yanzhao Yang, Qi Xie, Chengcai Chu, Hongning Tong
Summary: Through yeast two-hybrid screening, we identified a U-box ubiquitin ligase TUD1 that interacts with the negative regulator of BR signaling, GSK2, and promotes its degradation through ubiquitination. The tud1 mutant shows similar phenotypes to plants with constitutively activated GSK2, and GSK2 protein accumulates in the tud1 mutant. In addition, inhibition of BR synthesis promotes GSK2 accumulation and suppresses TUD1 stability, while BRs induce GSK2 degradation and promote TUD1 accumulation. Our study reveals the role of TUD1 in BR-induced GSK2 degradation and enhances our understanding of a critical step in the BR signaling pathway in rice.
PLANT COMMUNICATIONS
(2023)
Article
Immunology
Simone Thomas, Hinrich Abken
Summary: Chimeric antigen receptors (CARs) in the second generation format provide two signals for T cell activation. However, CAR T cell persistence is limited and can be improved by supplementing cytokines as the third signal. Recent progress in understanding receptor signaling allows for the engineering of cytokines to selectively stimulate CAR T cells. We discuss strategies for engineering cytokine help intensified CAR (CHIC) T cells for adoptive cell therapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Ondrej Cerny, Camilla Godlee, Romina Tocci, Nancy E. Cross, Haoran Shi, James C. Williamson, Eric Alix, Paul J. Lehner, David W. Holden
Summary: The bacterium Salmonella enterica inhibits T cell immune response by affecting T cell activation, mainly through degradation of the CD97 protein to reduce contacts between antigen-presenting cells and T cells. Additionally, Salmonella effector SteD depletes mature MHCII molecules by enhancing ubiquitination of CD97, which affects dendritic cell-T cell interactions independent of its effect on MHCII.
Review
Immunology
Peng Liu, Liwei Zhao, Laurence Zitvogel, Oliver Kepp, Guido Kroemer
Summary: The search for immunostimulatory drugs in cancer immunotherapy can benefit from target-agnostic methods that screen agents for their functional impact on immune cells cultured in vitro without preconceived notions of their mode of action. A synthetic mini-immune system has been developed to confront stressed and dying cancer cells with dendritic cells (DCs) and CD8(+) T-cell hybridoma cells. This system can identify three types of immunostimulatory drugs: those that stimulate immunogenic cell death (ICD) of cancer cells, drugs that enhance DC response to ICD, and drugs that increase T cell effector function. Strategies to develop ICD enhancers are discussed, including ligands of pattern recognition receptors and immunometabolic modifiers, as well as methods for target deconvolution.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Chie Ishikawa, Naoki Mori
Summary: The study showed that pimozide has anti-ATL effects by inhibiting cell growth, inducing apoptosis, increasing ROS production, and suppressing several signaling pathways activities.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Mingyu He, Kate Roussak, Feiyang Ma, Nicholas Borcherding, Vince Garin, Mike White, Charles Schutt, Trine I. Jensen, Yun Zhao, Courtney A. Iberg, Kairav Shah, Himanshi Bhatia, Daniel Korenfeld, Sabrina Dinkel, Judah Gray, Alina Ulezko Antonova, Stephen Ferris, David Donermeyer, Cecilia Lindestam Arlehamn, Matthew M. Gubin, Jingqin Luo, Laurent Gorvel, Matteo Pellegrini, Alessandro Sette, Thomas Tung, Rasmus Bak, Robert L. Modlin, Ryan C. Fields, Robert D. Schreiber, Paul M. Allen, Eynav Klechevsky
Summary: The reduction of human CD1c+CD5+ dendritic cells (DCs) in melanoma-affected lymph nodes and the correlation between CD5 expression on DCs and patient survival have been observed. Activating CD5 on DCs enhances T cell priming and improves survival after immune checkpoint blockade (ICB) therapy. CD5+ DCs are essential for optimal ICB therapy.
Review
Biochemistry & Molecular Biology
Zishuo Chen, Minkai Song, Ting Wang, Jiawen Gao, Fei Lin, Hui Dai, Chao Zhang
Summary: This review provides a detailed overview of the ubiquitination role of circular RNA (circRNA) in the regulation mechanism of various diseases, and discusses its potential significance in disease diagnosis and prognosis.
Article
Immunology
Jie Wang, Lina Yang, Jialing You, Dada Wen, Bo Yang, Canhua Jiang
Summary: The study found that PDGF-BB can induce autophagy of oral mucosal fibroblasts through the interaction of Beclin-1 and PI3KC3, promoting cell proliferation, migration, and collagen synthesis. However, the inhibition of this interaction weakened the fibroblasts' activities. Overall, PDGF-BB regulates the biological behavior of oral mucosal fibroblasts by inducing autophagy through the Beclin-1 pathway.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Immunology
Elisa E. Sanchez, Maria Tello-Lafoz, Aixuan J. Guo, Miguel de Jesus, Yassmin A. Elbanna, Benjamin Y. Winer, Sadna Budhu, Eric Chan, Eric Rosiek, Taisuke Kondo, Justyn DuSold, Naomi Taylor, Gregoire Altan-Bonnet, Michael F. Olson, Morgan Huse
Summary: Cytotoxic T lymphocytes (CTLs) fight intracellular pathogens and cancer by identifying and destroying infected or transformed target cells. CTLs form a specialized cytotoxic immune synapse (IS) with a target and release toxic proteins to induce target cell death. After the IS dissolves, CTLs can search for other targets and phagocytes can clear the dead cells.
Article
Immunology
Baohua Liu, Xiaomin Miao, Jiangyun Shen, Liyan Lou, Kangmin Chen, Fuqi Mei, Meng Chen, Xian Su, Xue Du, Zhenhu Zhu, Weihong Song, Xu Wang
Summary: Diabetic kidney disease (DKD) is a common complication of diabetes, and the understanding of its molecular mechanisms is still incomplete. A recent study found that the expression of deubiquitinating enzyme USP25 was significantly upregulated in the kidneys of diabetic mice, and its absence worsened diabetes-induced renal dysfunction and fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)