Article
Environmental Sciences
John D. Hansen, Keith A. Loftin, Zachary Laughrey, Ondrej Adamovsky
Summary: Various stressors can have profound impacts on immunity to pathogens, with little research into the effects of cyanobacterial toxins on disease resistance in mammals. Recent studies suggest that freshwater cyanotoxins may alter disease susceptibility parameters for fish, wildlife, and human health through interaction with specific components of innate immunity. However, the results of this study indicate that the tested toxins did not directly interact with key pathogen recognition receptors for innate immunity in humans.
Article
Immunology
Lian-Yun Wu, Shao-Feng Liu, Guo-Hong Hua, Ru-Min Jia, Yan-Hong Yong, Ravi Gooneratne, Xiang-Hong Ju
Summary: Research on the Magang goose TLR5 gene revealed that it is widely expressed in various tissues, highly homologous in function, structure, and sequence with TLR5 of other species, and plays an important role in bacterial recognition.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Fisheries
Xue Yu, Liangge He, Songze Gao, Yuqi Liu, Jiachang Xu, Yulin Zhao, Haoran Lin, Yong Zhang, Danqi Lu
Summary: Toll-like receptor 5 (TLR5) has two subtypes in fish, the membrane-associated TLR5 (TLR5M) and soluble TLR5 (TLR5S). TLR5M in orange-spotted grouper recognizes flagellin and activates the NF-κB signaling pathway, while EcTLR5S negatively regulates this pathway. The NF-κB binding sites in the 5'-flanking region of TLR5M play a significant role in its gene expression and activation.
Article
Ophthalmology
Chun-Chieh Lai, Sung-Huei Tseng, Sheng-Min Hsu, Yin-Ting Huang, Chi-Chang Shieh
Summary: The study demonstrated that UVB exposure may lead to pterygium formation through the expression of TLR3 and activation of NF-kappa B.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Review
Cell Biology
Valerie Lannoy, Anthony Cote-Biron, Claude Asselin, Nathalie Rivard
Summary: Phosphatases are important regulators of TLRs signaling and may represent novel therapeutic targets to control pathogenic TLRs signaling.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Chemistry, Multidisciplinary
Kwansung Seo, Ji Hye Yoo, Jisu Kim, Sung Jun Min, Dong Nyoung Heo, Il Keun Kwon, Ho-Jin Moon
Summary: Ginseng-derived extracellular nanovesicles (GDNs) have the potential to inhibit osteoclast differentiation and may be used for the prevention and treatment of bone loss diseases.
Article
Cell Biology
Nicolas Melin, Daniel Sanchez-Taltavull, Rene Fahrner, Adrian Keogh, Michel Dosch, Isabel Buechi, Yitzhak Zimmer, Michaela Medova, Guido Beldi, Daniel M. Aebersold, Daniel Candinas, Deborah Stroka
Summary: The study found that CBLB502 reduces liver damage in ischemia-reperfusion injury and ConA-induced immuno-hepatitis by activating NF-kappa B and STAT3 signaling pathways. IL-22 enhances CBLB502's protective effects through cooperation, increasing hepatocyte resistance to acute liver injury.
CELL DEATH & DISEASE
(2021)
Article
Environmental Sciences
Yinbiao Wang, Rui Zhang, Fuyun Yang, Lin Yang, Qingmei Li, Junqing Guo, Xiao Liu, Jie Song, Guofu Zhang, Juan Li, Zhen An, Neil E. Alexis, Ilona Jaspers, Weidong Wu
Summary: This study investigated the effects of PM2.5 exposure on influenza virus (H3N2) infection and the subsequent modulation of inflammation and antiviral immune response in human bronchial epithelial cells. The results showed that PM2.5 exposure alone increased pro-inflammatory cytokines but decreased the production of antiviral cytokine IFN-beta. Importantly, prior exposure to PM2.5 enhanced subsequent H3N2 infectivity, expression of viral protein, and upregulation of pro-inflammatory cytokines. Additionally, inhibition of NF-kappa B suppressed cytokine production induced by PM2.5 and H3N2.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Veterinary Sciences
Jaeeun Ko, Jaehwan Kim, Yang-Kyu Choi, Sang-Soep Nahm, Jayon Kim, Sun-Min Seo, Jin-Seok Seo, Woojong Lee, Weon Kuu Chung, Kidong Eom
Summary: This study evaluated the clinical safety and radiomitigative effect of KMRC011 in beagle dogs with radiation-induced oral mucositis. The results suggest that KMRC011 may be used as an adjuvant for short-term radiotherapy without severe adverse effects.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Akira Nakazato, Mai Mochizuki, Rie Shibuya-Takahashi, Haruna Fujimori, Keitaro Fujii, Satoshi Saijoh, Shinkichi Morita, Tomoko Yamazaki, Takayuki Imai, Ikuro Sato, Kennichi Satoh, Kazunori Yamaguchi, Kazuo Sugamura, Jun Yasuda, Kazuto Matsuura, Hideo Shojaku, Yukinori Asada, Keiichi Tamai
Summary: This study found that RELA, a subunit of nuclear factor kappaB, is critical for the transcription of CD271 in hypopharyngeal cancer cells. RELA promotes CD271 transcription in squamous cell carcinoma cell lines, but not in normal epithelium and neuroblastoma cell lines. The specific region + 957 to + 1138 within the CD271 promoter sequence is important for RELA binding.
SCIENTIFIC REPORTS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jingfei Fu, Lu Jia, Lili Wu, Yiyang Jiang, Rui Zhao, Juan Du, Lijia Guo, Chen Zhang, Junji Xu, Yi Liu
Summary: This study aimed to investigate the role of ultrasonicated Lactobacillus rhamnosus extract in osteoclast differentiation and its underlying mechanism, providing new strategies for the treatment of periodontitis. The effects of the LGG extract on osteoclast differentiation were evaluated, and the related signaling pathways were examined using western blotting. The ultrasonicated extract of Lactobacillus rhamnosus inhibited osteoclast differentiation by suppressing the activation of the NF-kappa B/c-Fos/NFATc1 pathway.
Article
Dentistry, Oral Surgery & Medicine
Jiahao Zeng, Ni Jia, Chunlan Ji, Sulan Zhong, Qiaoxue Chai, Chuan Zou, Lei Chen
Summary: This study aimed to evaluate the influence of experimental periodontitis on renal damage in obese rats. The results showed that periodontitis induced dysfunction and structural destruction of the kidney in obese rats, while plaque control relieved renal damage.
Article
Immunology
Hisatake Matsumoto, Brendon P. Scicluna, Kin Ki Jim, Fahimeh Falahi, Wanhai Qin, Berke Gurkan, Erik Malmstrom, Mariska T. Meijer, Joe M. Butler, Hina N. Khan, Tsuyoshi Takagi, Shunsuke Ishii, Marcus J. Schultz, Diederik van de Beek, Alex F. de Vos, Cornelis van 't Veer, Tom van der Poll
Summary: The study indicates that HIVEP1 acts as a negative regulator of NF-κB in monocytes, inhibiting the proinflammatory response induced by bacterial agonists. Dysregulation of HIVEP1 leads to significant changes in the monocyte transcriptome and modulation of NF-κB responsive genes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Kai Ning Ma, Yao Zhang, Zhao Yu Zhang, Bo Ning Wang, Yan Yan Song, Lu Lu Han, Xin Zhuo Zhang, Shao Rong Long, Jing Cui, Zhong Quan Wang
Summary: This study demonstrates that Tsgal protein binds to TLR-4 and activates an inflammatory pathway, mediating the invasion of T. spiralis larvae in the gut. These findings suggest that Tsgal could be a potential molecular target for a vaccine against the enteral invasive stage of T. spiralis.
VETERINARY RESEARCH
(2023)
Article
Microbiology
Jason M. Shapiro, Marcel R. de Zoete, Noah W. Palm, Yaro Laenen, Rene Bright, Meaghan Mallette, Kevin Bu, Agata A. Bielecka, Fang Xu, Andres Hurtado-Lorenzo, Samir A. Shah, Judy H. Cho, Neal S. LeLeiko, Bruce E. Sands, Richard A. Flavell, J. C. Clemente
Summary: The immunopathogenesis of inflammatory bowel disease (IBD) is linked to host genetics and intestinal dysbiosis. Investigating IgA responses to microbiota can reveal potential disease-modifying taxa and improved biomarkers of clinical course in IBD.
CELL HOST & MICROBE
(2021)
Article
Gastroenterology & Hepatology
Eelco C. Brand, Marjolein A. Y. Klaassen, Ranko Gacesa, Arnau Vich Vila, Hiren Ghosh, Marcel R. de Zoete, Dorret Boomsma, Frank Hoentjen, Carmen S. Horjus Talabur Horje, Paul C. van de Meeberg, Gonneke Willemsen, Jingyuan Fu, Cisca Wijmenga, Femke van Wijk, Alexandra Zhernakova, Bas Oldenburg, Rinse K. Weersma
Summary: The gut microbiome of healthy cotwins from IBD-discordant twin pairs displays IBD-like signatures, potentially preceding the onset of IBD. However, longitudinal follow-up studies are needed to infer a causal relationship.
Article
Microbiology
Xinyue Li, Richard W. Wubbolts, Nancy M. C. Bleumink-Pluym, Jos P. M. van Putten, Karin Strijbis
Summary: MUC1 at the host-microbe interface facilitates bacterial invasion through beta 1 integrins, involving a concerted action of the MUC1 O-glycosylated extracellular domain and cytoplasmic tail.
Article
Immunology
R. Villarreal, H. S. Manzer, A. M. Keestra-Gounder, K. S. Doran
Summary: The study on the pathogenesis of GBS meningitis reveals the critical roles of vimentin and NOD2 in mediating inflammatory responses induced by GBS. Vimentin is essential for regulating the inflammation process, while NOD2 promotes chemokine induction in response to GBS infection, highlighting their importance in host immune responses against GBS invasion.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Jiannan Cui, Coco Duizer, Lieneke Bouwman, Kristel S. van Rooijen, Carlos G. P. Voogdt, Jos P. M. van Putten, Marcel R. de Zoete
Summary: Authors identified ADP-heptose and/or related heptose phosphates released by Campylobacter jejuni as a potent activator of inflammation in intestinal epithelial cells, signaling through the ALPK1 receptor. These results suggest a potential therapeutic target to treat Campylobacter infection and elucidate the virulence mechanisms involved in the development of bacterial enteritis.
Article
Microbiology
Mostafa Asadpoor, Georgia-Nefeli Ithakisiou, Jos P. M. van Putten, Roland J. Pieters, Gert Folkerts, Saskia Braber
Summary: AOS and COS affect the growth of GBS V and S. aureus wood 46, functioning as anti-biofilm agents. The combination of AOS and COS with different antibiotics may provide new opportunities to combat antimicrobial resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Lydia A. Sweet, Sharon K. Kuss-Duerkop, A. Marijke Keestra-Gounder
Summary: ER stress and the IRE1 alpha pathway play crucial roles in inducing inflammation during bacterial infection.
INFECTION AND IMMUNITY
(2022)
Article
Engineering, Biomedical
Yang Cai, Jos P. M. S. van Putten, Myrthe S. Gilbert, Walter J. J. Gerrits, Gert Folkerts, Saskia Braber
Summary: The emergence of antimicrobial resistance in infections has created a need for new intervention strategies. Galacto-oligosaccharides (GOS) show potential as an alternative to antibiotics due to their anti-inflammatory and anti-adhesive properties. Mannheimia haemolytica is a major bacteria associated with bovine lung infections, and the study demonstrates that GOS can reduce its viability and enhance the efficacy of antibiotics. GOS also exhibit anti-adhesive and anti-invasive activities in primary bronchial epithelial cells, which can be attributed to their downregulation of toll-like receptor 4/nuclear factor-kappa B pathway. Furthermore, GOS have been shown to relieve lung infections/inflammation and reduce M. haemolytica positivity in vivo without altering clinical performance. These findings highlight the anti-inflammatory mechanisms of GOS and its potential as a promising agent for preventing infections.
Article
Multidisciplinary Sciences
M. M. Kroes, A. Miranda-Bedate, R. H. J. Jacobi, E. van Woudenbergh, G. den Hartog, J. P. M. van Putten, J. de Wit, E. Pinelli
Summary: This study investigated the response of human airway epithelial cells to Bordetella pertussis infection. The infection resulted in reduced epithelial barrier integrity and disrupted mucociliary transport. The study suggests that B. pertussis only induces mild immunological activation in the epithelial cells and requires communication with local immune cells for a broad immune response.
SCIENTIFIC REPORTS
(2022)
Article
Gastroenterology & Hepatology
Xuefeng Cao, Chris H. A. van de Lest, Liane Z. X. Huang, Jos P. M. van Putten, Marc M. S. M. Wosten
Summary: Research reveals that lysophospholipids of Campylobacter jejuni have toxicity to host cells, especially the short-chain lysoPEs (C:14) may be considered as a novel virulence factor.
Article
Microbiology
Maitrayee Chatterjee, Liane Z. X. L. Huang, Anna Mykytyn, Chunyan Wang, Mart B. Lamers, Bart Westendorp, Richard B. Wubbolts, Jos P. M. van Putten, Berend-Jan B. Bosch, Bart Haagmans, Karin B. Strijbis
Summary: In this study, the role of host mucins and mucin glycans on SARS-CoV-2 entry into airway epithelial cells was investigated. The removal of mucins from the surface of cells increased the binding of the virus and enhanced infection. This study demonstrates the important role of glycosylated extracellular mucin domains in the entry of SARS-CoV-2.
Article
Gastroenterology & Hepatology
Noortje Ijssennagger, Kristel S. van Rooijen, Stefania Magnusdottir, Jose M. Ramos Pittol, Ellen C. L. Willemsen, Marcel R. de Zoete, Matthijs J. D. Baars, Paul B. Stege, Carolina Colliva, Roberto Pellicciari, Sameh A. Youssef, Alain de Bruin, Yvonne Vercoulen, Folkert Kuipers, Saskia W. C. van Mil
Summary: This study highlights the importance of liver-to-gut communication for intestinal health, with a focus on colon functioning. Elimination of Fxr in the liver significantly affects colonic gene expression and enhances the protective capacity of the mucus barrier.
Article
Gastroenterology & Hepatology
Guus H. van Muijlwijk, Guido van Mierlo, Pascal W. T. C. Jansen, Michiel Vermeulen, Nancy M. C. Bleumink-Pluym, Noah W. Palm, Jos P. M. van Putten, Marcel R. de Zoete
Summary: The human gut microbiota plays a key role in intestinal health and disease, with some bacteria linked to human diseases exploiting the inner mucus layer as an important niche. A newly identified IBD-associated species, Allobaculum mucolyticum, was found to carry up to 60 genes encoding putative Carbohydrate Active Enzymes (CAZymes) that may contribute to mucosal colonization. Mass spectrometry revealed the presence of 49 CAZymes, with 26 significantly enriched in its secretome, suggesting that A. mucolyticum's CAZyme secretion may facilitate bacterial colonization and degradation of the mucus layer.